GAO Feiyan,CAO Yue,AI Hao,et al.Effects of rice ferroxidase gene OsLPR5 on root growth and nutrient utilization in Arabidopsis plants[J].Journal of Nanjing Agricultural University,2018,41(6):1054-1060.[doi:10.7685/jnau.201803030]





Effects of rice ferroxidase gene OsLPR5 on root growth and nutrient utilization in Arabidopsis plants
高飞燕1 曹越2 艾昊1 刘秀丽1 杨晶1 冯冰1 徐国华1 孙淑斌1
1. 南京农业大学作物遗传与种质创新国家重点实验室, 江苏 南京 210095;
2. 南京大学环境学院, 江苏 南京 210093
GAO Feiyan1 CAO Yue2 AI Hao1 LIU Xiuli1 YANG Jing1 FENG Bing1 XU Guohua1 SUN Shubin1
1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;
2. School of the Environment, Nanjing University, Nanjing 210093, China
OsLPR5 geneatlpr1 mutantrootnutrient
[Objectives] This work is aimed at confirming the effects of rice ferroxidase gene OsLPR5 on root growth and nutrient utilization in Arabidopsis plants, so as to investigated its function. [Methods] We analysed the ferroxidase activity of OsLPR5 protein expressed in bacteria by specific activity analysis. We obtained transgenic Arabidopsis plants overexpressing OsLPR5 in the atlpr1 background using the Agrobacterium-mediated floral dip method, and examined the impact of OsLPR5 overexpression on root morphology and nutrient utilization known to be related to phosphorous nutrition or its regulation. [Results] Ferroxidase activity of pGS-21a-OsLPR5 protein was significantly higher than the control. Under the condition of low phosphorus, the primary roots of OsLPR5 transformed lines became shorter, and the contents of total phosphorus, nitrogen, potassium, sodium, iron, copper and zinc decreased, which tended to wild type. The expression of OsLPR5 significantly compensated for the effect of AtLPR1 mutation on primary root phenotype and nutrient content, indicating that OsLPR5 plays an important role in root architecture and nutrient utilization. [Conclusions] The results confirm that OsLPR5 protein probably has ferroxidase activity, and indicate that OsLPR5 is probably involved in processes, including root morpholog and nutrient utilization to environmental phosphorus stress.


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更新日期/Last Update: 2018-11-23