[1]杨春,李惠芳,杨树,等.母鼠妊娠期和哺乳期日粮添加甜菜碱对子代雌性大鼠机体铁代谢的影响及其机制[J].南京农业大学学报,2018,41(6):1100-1106.[doi:10.7685/jnau.201711039]
 YANG Chun,LI Huifang,YANG Shu,et al.Effects of maternal betaine supplementation during gestagation and lactation on iron metablism in female offsprings of rats and its mechanism[J].Journal of Nanjing Agricultural University,2018,41(6):1100-1106.[doi:10.7685/jnau.201711039]
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母鼠妊娠期和哺乳期日粮添加甜菜碱对子代雌性大鼠机体铁代谢的影响及其机制()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年6期
页码:
1100-1106
栏目:
出版日期:
2018-11-25

文章信息/Info

Title:
Effects of maternal betaine supplementation during gestagation and lactation on iron metablism in female offsprings of rats and its mechanism
作者:
杨春 李惠芳 杨树 方茜 马文强 赵茹茜
南京农业大学农业农村部动物生理生化重点实验室/动物医学院, 江苏 南京 210095
Author(s):
YANG Chun LI Huifang YANG Shu FANG Xi MA Wenqiang ZHAO Ruqian
Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing 210095, China
关键词:
甜菜碱大鼠铁代谢
Keywords:
betaineratsiron metabolism
分类号:
S813.24
DOI:
10.7685/jnau.201711039
文献标志码:
A
摘要:
[目的]本文旨在研究母鼠日粮饲喂甜菜碱对子代雌性大鼠机体铁代谢的影响及其机制。[方法]随机选取56只10周龄Sprague Dawley雌性大鼠,与同种雄鼠自然交配,配种成功后,孕鼠随机分为对照组和甜菜碱组。对照组孕鼠在怀孕第1~21天饲喂基础日粮,甜菜碱组孕鼠同期饲喂在基础日粮上添加质量分数为1%甜菜碱的混合日粮。出生雌性大鼠于(3周龄)断奶后饲喂基础日粮至16周龄。记录13~16周龄子代雌性大鼠生产性能,测定16周龄子代雌性大鼠血液及组织铁代谢相关指标并分析其肝脏铁代谢相关基因及蛋白的表达。[结果]与对照相比,母体添加甜菜碱对子代雌性大鼠生长性能无明显影响,但显著或极显著降低16周龄子代雌性大鼠的血红蛋白含量(P<0.05)、血清铁含量、转铁蛋白结合铁含量和总铁结合力(P<0.01),促进肝脏铁摄取相关蛋白二价金属离子转运体(DMT1)及转铁蛋白受体1(TFR1)蛋白表达(P<0.05)。[结论]母体饲喂甜菜碱降低了子代雌性大鼠血清铁水平,但对肝脏铁含量没有显著影响。肝脏铁的稳态与甜菜碱诱导的肝脏铁摄取通路相关蛋白(TFR1和DMT1)的高表达密切相关。
Abstract:
[Objectives] This paper aimed to study the effects of maternal dietary betaine supplementation during gestagation and lactation on the iron metabolism in female offspring of rats. [Methods] Fifty-six 10 week-old Sprague Dawley female rats were randomly selected, mated and divided into two groups:control group (Con)and betaine group (Bet). Female rats were fed with basal diet and trail diet (basal diet plus 1% betaine)during pregnancy and lactation, respectively. The female offsprings of rats were selected and provided with basal diet from weaning up to 16-weeks. The growth performance was recorded during the experimental periods. The blood iron parameters, tissue iron content and hepatic iron metabolism related genes and protein expression were measured at the end of the experiment. [Results] Maternal betaine supplementation had no significant effect on growth performance, greatly decreased hemoglobin concentration, serum iron, transferrin-bound iron and total iron binding capacity (P<0.01), but significantly enhanced hepatic iron-uptake-related proteins DMT1 (divalent metal transperfer 1)and TFR1 (transferrin receptor protein 1)expression (P<0.05). [Conclusions] Maternal betaine exposure during pregnancy and lactation decreases serum iron content, but does not affect hepatic iron content in female offsprings of rats. Maintenance of hepatic iron homeostasis is strong associated with high expression of DMT1 and TFR1 induced by betaine in female offsprings of rats.

参考文献/References:

[1] Alwan N A,Hamamy H. Maternal iron status in pregnancy and long-term health outcomes in the offspring[J]. Journal of Pediatric Genetics,2015,4 (2):111-123.
[2] Puntarulo S. Iron,oxidative stress and human health[J]. Molecular Aspects of Medicine,2005,26 (4/5):299-312.
[3] Finberg K E. Regulation of systemic iron homeostasis[J]. Current Opinion in Hematology,2013,20 (3):208-214.
[4] Ganz T. Systemic iron homeostasis[J]. Physiological Reviews,2013,93 (4):1721-1741.
[5] Ganesan B,Anandan R,Lakshmanan P T. Studies on the protective effects of betaine against oxidative damage during experimentally induced restraint stress in Wistar albino rats[J]. Cell Stress and Chaperones,2011,16 (6):641-652.
[6] Bingul I,Basaran-Kucukgergin C,Aydin A F,et al. Betaine treatment decreased oxidative stress,inflammation,and stellate cell activation in rats with alcoholic liver fibrosis[J]. Environmental Toxicology Pharmacology,2016,45:170-178.
[7] Ge C X,Yu R,Xu M X,et al. Betaine prevented fructose-induced NAFLD by regulating LXRalpha/PPARalpha pathway and alleviating ER stress in rats[J]. European Journal of Pharmacology,2016,770:154-164.
[8] Xu L,Huang D P,Hu Q L,et al. Betaine alleviates hepatic lipid accumulation via enhancing hepatic lipid export and fatty acid oxidation in rats fed with a high-fat diet[J]. British Journal of Nutrition,2015,114 (6):995-996.
[9] Kettunen H,Peuranen S,Tiihonen K. Betaine aids in the osmoregulation of duodenal epithelium of broiler chicks,and affects the movement of water across the small intestinal epithelium in vitro[J]. Comparative Biochemistry and Physiology,2001,129 (2):595-603.
[10] Kettunen H,Tiihonen K,Peuranen S,et al. Dietary betaine accumulates in the liver and intestinal tissue and stabilizes the intestinal epithelial structure in healthy and coccidia-infected broiler chicks[J]. Comparative Biochemistry and Physiology A,2001,130 (4):759-769.
[11] Tran P V,Kennedy B C,Pisansky M T,et al. Prenatal choline supplementation diminishes early-life iron deficiency-induced reprogramming of molecular networks associated with behavioral abnormalities in the adult rat hippocampus[J]. British Journal of Nutrition,2016,146 (3):484-493.
[12] Kennedy B C,Dimova J G,Siddappa A J,et al. Prenatal choline supplementation ameliorates the long-term neurobehavioral effects of fetal-neonatal iron deficiency in rats[J]. British Journal of Nutrition,2014,144 (11):1858-1865.
[13] Kennedy B C,Tran P V,Kohli M,et al. Beneficial effects of postnatal choline supplementation on long-term neurocognitive deficit resulting from fetal-neonatal iron deficiency[J]. Behavioural Brain Research,2018,336:40-43.
[14] Hogeveen M,den Heijer M,Semmekrot B A,et al. Umbilical choline and related methylamines betaine and dimethylglycine in relation to birth weight[J]. Pediatric Research,2013,73 (6):783-787.
[15] Lever M,Slow S. The clinical significance of betaine,an osmolyte with a key role in methyl group metabolism[J]. Clinical Biochemistry,2010,43 (9):732-744.
[16] Dominguez-Salas P,Moore S E,Cole D,et al. DNA methylation potential:dietary intake and blood concentrations of one-carbon metabolites and cofactors in rural African women[J]. The American Journal of Clinical Nutrition,2013,97 (6):1217-1227.
[17] Molloy A M,Mills J L,Cox C,et al. Choline and homocysteine interrelations in umbilical cord and maternal plasma at delivery[J]. American Journal of Clinical Nutrition,2005,82 (4):836-842.
[18] van Lee L,Tint M T,Aris I M,et al. Prospective associations of maternal betaine status with offspring weight and body composition at birth:the GUSTO (growing up in Singapore Towards Healthy Outcomes)cohort study[J]. The American Journal of Clinical Nutrition,2016,104 (5):1327-1333.
[19] Cai D,Jia Y,Lu J,et al. Maternal dietary betaine supplementation modifies hepatic expression of cholesterol metabolic genes via epigenetic mechanisms in newborn piglets[J]. British Journal of Nutrition,2014,112 (9):1459-1468.
[20] Cai D,Jia Y,Song H,et al. Betaine supplementation in maternal diet modulates the epigenetic regulation of hepatic gluconeogenic genes in neonatal piglets[J]. PLoS One,2014,9 (8):e105504.
[21] Udali S,Castagna A,Corbella M,et al. Hepcidin and DNA promoter methylation in hepatocellular carcinoma[J]. European Journal of Clinical Investigation,2018,48 (2):e12870.
[22] 王换换,申正杰,肖航,等. 热应激对肝脏中Keap1-Nrf2信号通路及下游基因表达的影响[J]. 南京农业大学学报,2017,40 (1):151-156. DOI:10.7685/janu.201602023. Wang H H,Shen Z J,Xiao H,et al. Effects of heat stress on Keap1-Nrf2-ARE signal pathway of liver in dairy cows[J]. Journal of Nanjing Agricultural University,2017,40 (1):151-156 (in Chinese with English abstract).
[23] 高志参,李照耀,周静,等. 4个种属Mx蛋白抑制水疱性口炎病毒的研究[J]. 南京农业大学学报,2017,40 (4):690-696. DOI:10.7685.janu.201703023. Gao Z C,Li Z Y,Zhou J,et al. Research of Mx proteins from four species against vesicular stomatitis virus[J]. Journal of Nanjing Agricultural University,2017,40 (4):690-696 (in Chinese with English abstract).
[24] 冯杰,余东游. 甜菜碱对生长肥育猪生长性能的影响及其转甲基效应研究[J]. 中国畜牧杂志,2001,37 (3):8-10. Feng J,Yu D Y. Effect of betaine on growth performance and methyl transfer function in growing-finishing swine[J]. Chinese Journal of Animal Science,2001,37 (3):8-10 (in Chinese with English abstract).
[25] Yu D,Xu Z. Effects of betaine on growth performance and carcass characteristics in growing pigs[J]. Asian-Australasian Journal of Animal Sciences,2003,17 (12):490-493.
[26] 郭建凤,武英,呼红梅. 甜菜碱对育肥猪生长性能及胴体的肉质影响[J]. 家畜生态学报,2007,28 (2):21-24. Guo J F,Wu Y,Hu H M. Effect of lycine on growth performance and meat qualities of carcass of pigs[J]. Journal of Domestic Animal Ecology,2007,28 (2):21-24 (in Chinese with English abstract).
[27] 王俊东,李俊平,张建峰,等. 不同时期添加甜菜碱对肉鸡生产性能和脂肪代谢的影响[J]. 中国兽医学报,2004,24 (1):87-91. Wang J D,Li J P,Zhang J F,et al. Effect of betaine on broiler product performance and fat metabolism in different stage supplement[J]. Chinese Journal of Veterinary Science,2004,24 (1):87-91 (in Chinese with English abstract).
[28] 马建爽,常文环,张姝,等. 甜菜碱对肉鸡生长性能、脂质代谢及肌肉风味品质的影响[J]. 动物营养学报,2015,27 (1):185-195. Ma J S,Chang W H,Zhang S,et al. Effects of betaine on growth performance,lipid metabolism and flavor quality in muscle of broilers[J]. Chinese Journal of Animal Nutrition,2015,27 (1):185-195 (in Chinese with English abstract).
[29] 卢霞,王君,王颢,等. 甜菜碱对高脂性肥胖大鼠体重和生化指标的影响[J]. 营养学报,2008,30 (3):311-315. Lu X,Wang J,Wang H,et al. Effects of betaine on body weight and biochemical index in obese rats fed high-fat diet[J]. Acta Nutrimenta Sinica,2008,30 (3):311-315 (in Chinese with English abstract).
[30] Hu Y,Sun Q,Zong Y,et al. Prenatal betaine exposure alleviates corticosterone-induced inhibition of CYP27A1 expression in the liver of juvenile chickens associated with its promoter DNA methylation[J]. General and Comparative Endocrinology,2017,246:241-248.
[31] Slow S,Lever M,Chambers S T,et al. Plasma dependent and independent accumulation of betaine in male and female rat tissues[J]. Physiological Research,2009,58 (3):403-410.
[32] Zhao N,Yang S,Jia Y,et al. Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats[J]. The Journal of Nutritional Biochemistry,2018,54:105-112.
[33] Anderson E R,Shah Y M. Iron homeostasis in the liver[J]. Comprehensive Physiology,2013,3 (1):315-330.
[34] Barisani D,Conte D. Transferrin receptor 1 (TfR1)and putative stimulator of Fe transport (SFT)expression in iron deficiency and overload:an overview[J]. Blood Cells Molecules and Diseases,2002,29 (3):498-505.

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备注/Memo

备注/Memo:
收稿日期:2017-11-28。
基金项目:国家重点研发计划项目(2016YFD0500500)
作者简介:杨春,硕士研究生。
通信作者:马文强,副教授,研究方向为畜禽营养生理调控,E-mail:wq8110@njau.edu.cn。
更新日期/Last Update: 2018-11-23