[1]王伟科,宋吉玲,闫静,等.秀珍菇转录组测序和初步分析[J].南京农业大学学报,2019,42(2):292-299.[doi:10.7685/jnau.201807003]
 WANG Weike,SONG Jiling,YAN Jing,et al.Transcriptome sequencing and analysis of Pleurotus pulmonarius[J].Journal of Nanjing Agricultural University,2019,42(2):292-299.[doi:10.7685/jnau.201807003]
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秀珍菇转录组测序和初步分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年2期
页码:
292-299
栏目:
生物与环境
出版日期:
2019-03-19

文章信息/Info

Title:
Transcriptome sequencing and analysis of Pleurotus pulmonarius
作者:
王伟科 宋吉玲 闫静 袁卫东 陆娜
杭州市农业科学研究院, 浙江 杭州 310024
Author(s):
WANG Weike SONG Jiling YAN Jing YUAN Weidong LU Na
Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China
关键词:
秀珍菇菌丝体子实体转录组测序高通量测序技术
Keywords:
Pleurotus pulmonariusmyceliumfruiting bodytranscriptome sequencinghigh-throughput sequencing technology
分类号:
S646;Q78
DOI:
10.7685/jnau.201807003
摘要:
[目的] 本文旨在了解秀珍菇子实体形成的分子机制,为培育优质、高产、抗逆的秀珍菇品种提供理论基础。[方法] 构建秀珍菇高质量cDNA文库,采用illumina高通量测序技术对秀珍菇菌丝体和子实体进行转录组测序,并利用生物信息学方法开展基因表达谱的研究以及功能基因的预测。[结果] 共获得81 693个非重复序列基因(universal gene,UniGene),经BLAST比对NR数据库,可比对上的UniGene数量为61 306个。NR数据分析显示,秀珍菇已比对的UniGene与糙皮侧耳相似度最高,占总数目的86.1%。同时,对秀珍菇转录组的UniGene进行了生物学通路的注释和预测,共注释14 315个UniGene,且部分与糖类代谢、氨基酸代谢、信号传导、翻译相关通路有关,分别为1 683、1 241、1 243和1 372个,表明富集在上述通路中的UniGene在秀珍菇子实体形成过程中可能起到重要作用。通过分析SNP突变,发现秀珍菇中分别有10 967和10 683个位点发生C/T转换和A/G转换,同时,有2 102个位点发生A/C颠换。通过查找分析SSR位点发现,共获得7 574个SSR位点,占UniGene总数的比例为9.27%。其中,单核苷酸重复在所有SSR重复类型中所占比例最高,为45.14%,其次为三核苷酸重复类型,为31.94%。[结论] 通过高通量测序技术获得秀珍菇菌丝体和子实体的转录组数据,结合生物信息学分析,为了解秀珍菇群体遗传多样性、构建遗传连锁图谱和研究其不同生长阶段差异表达基因及其功能奠定基础。
Abstract:
[Objectives] This paper aimed to understand the molecular mechanism of fruiting body formation of Pleurotus pulmonarius and provide theoretical basis for breeding high-quality,high-yield and stress-resistant varieties of P.pulmonarius.[Methods] In this study,on the basis of building high quality cDNA library of P.pulmonarius,the transcriptome of mycelium and fruiting body of P.pulmonarius was sequenced by illumina high-throughput sequencing technology. In addition,bioinformatics methods were used to study gene expression profiles and predict functional genes.[Results] It showed that 81 693 UniGenes were obtained through sequence splicing and 61 306 UniGenes were annotated through NR database by BLAST comparison. By statistical species distribution,P.pulmonarius transcriptome had the most similar UniGenes to Pleurotus ostreatus. Meanwhile,a total number of 14 315 UniGenes were identified by annotating and forecasting the biological pathways for the transcriptome of P.pulmonarius. Among 14 315 UniGenes,parts of their biological functions were related to carbohydrate metabolism(1 683),amino acid metabolism(1 241),signal transduction(1 243) and translation(1 372). It was noted that these UniGenes enriched in afore-mentioned pathway might play a very important role in the process of fruiting body formation of P.pulmonarius. 10 967 C/T and 10 683 A/G conversion,2 102 A/C transversion were found by SNP analysis. Meanwhile,7 574 SSR in 81 693 UniGenes were found which took 9.27% of the total number of UniGenes. The characteristic of SSR distribution showed that mono-nucleotide repeat was the highest,which was 45.14% of the total,followed by tri-nucleotide repeat,which was 31.94%.[Conclusions] The transcription group data were obtained of the mycelium and fruiting body of P.pulmonarius by high-throughput sequencing technology. Combined bioinformatics analysis,this study laid the foundation for analysing the genetic diversity,constructing genetic linkage map,and researching the differentially expressed genes and its functions at different developmental phases of P.pulmonarius.

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相似文献/References:

[1]陈济琛,郑永标,林新坚,等.姬松茸菌丝及担孢子核观察[J].南京农业大学学报,2005,28(4):144.[doi:10.7685/j.issn.1000-2030.2005.04.032]

备注/Memo

备注/Memo:
收稿日期:2018-7-1。
基金项目:浙江省农业(食用菌)新品种选育重大科技专项(2016C02057)
作者简介:王伟科,高级农艺师,主要从事食用菌新品种选育与栽培技术研究,E-mail:akeok@126.com。
通信作者:王伟科,高级农艺师,主要从事食用菌新品种选育与栽培技术研究,E-mail:akeok@126.com。
更新日期/Last Update: 1900-01-01