[1]高懿,黄金虎,陈丽,等.江苏北部地区猪源屎肠球菌耐药性分析及其酰胺醇类耐药基因的检测[J].南京农业大学学报,2019,42(2):322-327.[doi:10.7685/jnau.201806024]
 GAO Yi,HUANG Jinhu,CHEN Li,et al.Antimicrobial susceptibility and amphenicols resistance genes in Enterococcus faecium isolated from swine in the northern of Jiangsu Province[J].Journal of Nanjing Agricultural University,2019,42(2):322-327.[doi:10.7685/jnau.201806024]
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江苏北部地区猪源屎肠球菌耐药性分析及其酰胺醇类耐药基因的检测()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年2期
页码:
322-327
栏目:
动物科学
出版日期:
2019-03-19

文章信息/Info

Title:
Antimicrobial susceptibility and amphenicols resistance genes in Enterococcus faecium isolated from swine in the northern of Jiangsu Province
作者:
高懿 黄金虎 陈丽 王梦莉 孙俊杰 王丽平
南京农业大学动物医学院, 江苏 南京 210095
Author(s):
GAO Yi HUANG Jinhu CHEN Li WANG Mengli SUN Junjie WANG Liping
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
关键词:
屎肠球菌耐药性酰胺醇类耐药基因
Keywords:
Enterococcus faeciumsusceptibilityamphenicolsresistance genes
分类号:
R378.1
DOI:
10.7685/jnau.201806024
摘要:
[目的] 本试验分析了江苏北部地区2016-2017年分离的135株猪源屎肠球菌的耐药表型,并对其携带的酰胺醇类耐药基因fexA、fexB、floR、cfr、optrA进行检测,以了解猪源屎肠球菌的耐药现状及其对酰胺醇类抗生素的耐药机制,为抗菌药物的合理用药提供参考。[方法] 猪肛门拭子采样,分离、纯化并鉴定屎肠球菌后,采用微量肉汤稀释法检测菌株对12种抗菌药物的耐药表型,采用PCR方法检测酰胺醇类耐药基因在屎肠球菌中的分布情况。[结果] 分离的135株屎肠球菌对红霉素、四环素、氟苯尼考的耐药率较高,分别为99.26%、98.52%和82.22%,对利奈唑胺的耐药率最低,为2.96%,对其他药物的耐药率由高到低分别为氨苄西林(75.56%)、链霉素(74.07%)、庆大霉素(71.11%)、麻保沙星(69.63%)、青霉素G(45.19%)、利福平(34.07%)和磷霉素(20.00%);未发现万古霉素耐药菌株。结果还显示所有菌株都为多药耐药菌株,以7耐为主(39株),还有3株表现为10耐。111株氟苯尼考耐药的菌株中,耐药基因fexB、fexA、optrA、floRcfr的携带率分别为58.56%(65)、27.03%(30)、20.72%(23)、17.12%(19)和0.90%(1),部分菌株同时携带多种耐药基因。[结论] 本试验分离的猪源屎肠球菌均为多药耐药菌株,并对兽医专用抗生素氟苯尼考产生较高的耐药性。该结果为猪源肠球菌耐药性流行病学调查及其对酰胺醇类耐药机制的研究奠定基础,并为江苏地区猪场抗菌药的合理使用提供依据。
Abstract:
[Objectives] The aim of the study is to analyze the resistant phenotype of 135 strains of Enterococcus faecium isolated from the northern of Jiangsu Province during 2016-2017 and detect the amphenicols resistance genes fexA,fexB,floR,cfr and optrA for further knowing the prevalence of resistant E.faecium and the mechanisms involved in phenicols resistance.[Methods] E.faecium was isolated from swine anal swabs and further identified by PCR. The broth microdilution method was used to determine the minimal inhibitory concentration(MIC) of 12 antimicrobials against E.faecium. Amphenicols resistance genes fexB,fexA,optrA,floR,and cfr were detected by PCR in florfenicol resistant strains.[Results] Total of 135 strains of E.faecium were isolated from 300 samples. The resistant rates of erythromycin,tetracycline and florfenicol were the highest with rates of 99.26%,98.52% and 82.22%,respectively. The resistance rates of other drugs from high to low were ampicillin(75.56%),streptomycin(74.07%),gentamicin(71.11%),marbofloxacin(69.63%),penicillin G(45.19%),rifampicin(34.07%) and fosfomycin(20.00%),respectively. The strains had a lowest resistance toward linezolid and the rate was 2.96%. Vancomycin resistant strains were not found in this study. Further results showed that most of the strains presented multidrug resistance profiles. Among all the resistant strains,39 strains were resistant to 7 kinds of drugs and three strains showed resistance to 10 kinds of drugs. Among the 111 strains resistant to amphenicols,the carrying rates of the resistance genes fexB,fexA,optrA,floR,and cfr were 58.56%(65),27.03%(30),20.72%(23),17.12%(19),and 0.90%(1),respectively. Some of strains carried multiple drug resistance genes at the same time.[Conclusions] The clinical isolates of Enterococcus faecium from swine are resistant to commonly used antibacterial drugs,in particular,resistance to florfenicol is particularly serious and it should be payed attention to. The results of the study laid the foundations for clarifying the transmission mechanisms of antibiotic-resistant genes and further provided a guidance for reasonable use of antimicrobials in piggeries of Jiangsu Province.

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备注/Memo

备注/Memo:
收稿日期:2018-6-19。
基金项目:国家自然科学基金项目(31572567);国家重点研发计划项目(2018YFD0500300)
作者简介:高懿,硕士研究生。
通信作者:王丽平,教授,博导,研究方向为细菌耐药机制,E-mail:wlp71@163.com。
更新日期/Last Update: 1900-01-01