[1]吴怀远,赵楠,王艺光,等.菊花CmGATA12基因促进开花的分子机制[J].南京农业大学学报,2019,42(4):657-664.[doi:10.7685/jnau.201807045]
 WU Huaiyuan,ZHAO Nan,WANG Yiguang,et al.Molecular mechanism of CmGATA12 gene promoting flowering in chrysanthemum[J].Journal of Nanjing Agricultural University,2019,42(4):657-664.[doi:10.7685/jnau.201807045]
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菊花CmGATA12基因促进开花的分子机制()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年4期
页码:
657-664
栏目:
植物科学
出版日期:
2019-07-08

文章信息/Info

Title:
Molecular mechanism of CmGATA12 gene promoting flowering in chrysanthemum
作者:
吴怀远 赵楠 王艺光 刘伟鑫 蒋甲福 陈发棣
南京农业大学园艺学院/农业农村部景观设计重点实验室, 江苏 南京 210095
Author(s):
WU Huaiyuan ZHAO Nan WANG Yiguang LIU Weixin JIANG Jiafu CHEN Fadi
College of Horticulture/Key Laboratory of Landscape Agriculture, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing 210095, China
关键词:
菊花花期调控转录因子CmGATA12基因
Keywords:
chrysanthemumflowering regulationtranscription factorCmGATA12 gene
分类号:
S682.1
DOI:
10.7685/jnau.201807045
摘要:
[目的]本文旨在探究菊花CmGATA12基因的功能,分析其表达特征,并转入拟南芥中对其功能进行初步研究。[方法]从菊花品种‘清露’中克隆获得CmGATA12全长序列,与其他物种进行同源性比对分析,同时利用实时荧光定量PCR分析CmGATA12在菊花不同组织中的表达变化;将CmGATA12基因转入拟南芥中,观察其对拟南芥开花时间和莲座叶片数的影响,并分析CmGATA12转基因拟南芥中相关开花基因的表达变化。[结果]CmGATA12与拟南芥GATA家族的AtGATA13和AtGATA8的遗传距离最近。组织定量分析表明CmGATA12在菊花的茎中表达量最高,在芽中表达量最低。亚细胞定位和转录活性试验表明,CmGATA12定位在细胞核和细胞膜并且具有转录激活活性。统计CmGATA12转基因拟南芥植株的开花时间和莲座叶片数发现,CmGATA12转基因拟南芥植株的开花时间均提前于野生型拟南芥植株,开花时的莲座叶片数均低于野生型拟南芥植株。CmGATA12转基因拟南芥植株相关开花基因的表达中,促进开花的基因AtSPL5AtGICmGATA12转基因植株中较野生型拟南芥中的表达量上调,抑制开花的基因AtSVP、AtFRI、AtTOE1AtTOE2CmGATA12转基因植株中较野生型表达量下调。[结论]本研究克隆得到的菊花CmGATA12基因,具有促进拟南芥开花的功能。
Abstract:
[Objectives]The purpose of the current study was to characterize the function of chrysanthemum CmGATA12 gene by analyzing its expression profile in chrysanthemum and heterologous expression in Arabidopsis thaliana to gain more insight into the function of the protein.[Methods]The full-length cDNA of CmGATA12 was cloned from the chrysanthemum variety ‘Qinglu’ and compared with other GATA12 sequences from different species. Simultaneously,real-time quantitative PCR was used to analyze the expression changes of CmGATA12 gene in different tissues of chrysanthemum. The CmGATA12 gene was transfected into A.thaliana,and its effect on flowering time and rosette leaf number in A.thaliana was observed. The expression of flowering related genes in CmGATA12 transgenic A.thaliana was analyzed.[Results]The genetic distance between CmGATA12 and AtGATA13 and AtGATA8 of Arabidopsis GATA family was the closest. Quantitative analysis of CmGATA12 gene in different tissues of chrysanthemum revealed that CmGATA12 gene had the highest expression level in the stem of chrysanthemum and the lowest expression level in bud. Subcellular localization indicated that the CmGATA12 gene was located both on the cell membrane and on the nucleus. The CmGATA12 gene was found to have transcriptional activity in the yeast system. By observing the time taken to flowering and rosette leaf number of CmGATA12 transgenic A.thaliana plants,it was found that the flowering time of CmGATA12 transgenic A.thaliana plants was earlier than that of wild-type plants,and the number of rosette leaves in flowering was lower than that of wild-type plants. Flowering-time related genes involved in flowering promotion(AtSPL5 and AtGI)and flowering inhibition genes(AtSVP,AtFRI,AtTOE1 and AtTOE2)were compared in the transgenic and wild-type plants. The results indicated that flower-promoting genes were up-regulated in the transgenic plants compared to the wild-type plants. Similarly,floral-repression genes were reduced expressed in transgenic plants compared with wild-type plants.[Conclusions]CmGATA12 gene has a role in promoting flowering in the heterologous system.

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备注/Memo

备注/Memo:
收稿日期:2018-07-22。
基金项目:江苏省农业科技自主创新资金项目[CX(16)1025];国家自然科学基金项目(31572159);中央高校基本业务费专项资金(KYRC201601)
作者简介:吴怀远,硕士研究生。
通信作者:陈发棣,教授,博导,主要从事菊花遗传育种与分子生物学研究,E-mail:chenfd@njau.edu.cn。
更新日期/Last Update: 1900-01-01