[1]张元鹏,杨占娜,杨利,等.乙型脑炎病毒感染猪睾丸细胞的miRNA表达谱差异分析[J].南京农业大学学报,2019,42(4):734-739.[doi:10.7685/jnau.201812012]
 ZHANG Yuanpeng,YANG Zhanna,YANG Li,et al.Identification and analysis of the miRNA expression profiles of Japanese encephalitis virus(JEV)infected swine testis(ST)cells[J].Journal of Nanjing Agricultural University,2019,42(4):734-739.[doi:10.7685/jnau.201812012]
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乙型脑炎病毒感染猪睾丸细胞的miRNA表达谱差异分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年4期
页码:
734-739
栏目:
动物科学
出版日期:
2019-07-08

文章信息/Info

Title:
Identification and analysis of the miRNA expression profiles of Japanese encephalitis virus(JEV)infected swine testis(ST)cells
作者:
张元鹏1 杨占娜2 杨利1 李兰1 于晓明1 杜露平1 陈瑾1 侯立婷1 乔绪稳1 侯继波1 郑其升134
1. 江苏省农业科学院动物免疫工程研究所/国家兽用生物制品工程技术研究中心, 江苏 南京 210014;
2. 南京市栖霞区动物卫生监督所, 江苏 南京 210046;
3. 江苏省动物重要疫病与人兽共患病防控协同创新中心, 江苏 扬州 225009;
4. 江苏省食品质量安全重点实验室/省部共建国家重点实验室培育基地, 江苏 南京 210014
Author(s):
ZHANG Yuanpeng1 YANG Zhanna2 YANG Li1 LI Lan1 YU Xiaoming1 DU Luping1 CHEN Jin1 HOU Liting1 QIAO Xuwen1 HOU Jibo1 ZHENG Qisheng13
1. Institute of Veterinary Immunology & Engineering/National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. Qixia Animal Health Supervision Institute, Nanjing 210046, China;
3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China;
4. Jiangsu Provincial Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality/Province-State Key Laboratory Breeding Base, Nanjing 210014, China
关键词:
乙型脑炎病毒猪睾丸细胞miRNA高通量测序
Keywords:
Japanese encephalitis virus(JEV)swine testis(ST)cellsmiRNAhigh-throughput sequencing
分类号:
S855.3
DOI:
10.7685/jnau.201812012
摘要:
[目的]本试验旨在分析乙型脑炎病毒(JEV)感染猪睾丸(ST)细胞和正常ST细胞miRNA差异表达谱,以探索miRNA在JEV感染过程中的作用及其调控机制。[方法]分别提取正常ST细胞和JEV感染8 h后的ST细胞总RNA,利用高通量测序技术检测分析ST细胞中的miRNA表达谱并进行差异分析。使用miRanda软件对表达差异显著miRNA的靶基因进行预测并对靶基因进行GO分析。选取6个显著性差异表达的miRNA通过RT-qPCR进行验证。[结果]与正常ST细胞相比,JEV感染后的ST细胞有112个差异表达miRNA,其中80个miRNA上调表达,32个miRNA下调表达。经RT-qPCR方法验证6个差异表达的miRNA结果与高通量测序结果一致。差异表达miRNA靶基因的生物学功能相对集中在细胞代谢、细胞黏附等生物过程。[结论]检测和分析JEV感染后猪睾丸细胞的miRNA表达谱,获得了大量的与JEV感染相关的miRNA表达谱数据,为揭示JEV感染生殖系统致病机制提供了有效的数据和理论基础。
Abstract:
[Objectives]The paper aimed to detect microRNA(miRNA)expression profile in Japanese encephalitis virus(JEV)infected swine testis(ST)cells.[Methods]Total RNA of JEV-infected and uninfected ST cells was extracted individually by Trizol and then analyzed miRNA expression profiles by high-throughput sequencing technology. miRNA target prediction algorithm miRanda was employed to predict potential target genes of differently expressed miRNA. Gene ontology(GO)analysis was performed on the host target genes,and 6 signifacant differences in expression of miRNA were verified by quantitative real time polymerase chain reaction(RT-qPCR).[Results]The results indicated that total 112 miRNA were obviously differently expressed in the JEV-infected cells including 80 up-regulated miRNA and 32 down-regulated miRNA. The six of those miRNA were verified by RT-qPCR,the results were well matched with those of sequencing data. GO analysis of the target genes showed that these miRNA involved in the biological processes of cell metabolism and cell adhesion.[Conclusions]Our findings indicate that miRNA may play critical roles in the pathogenesis of JEV infection.

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备注/Memo

备注/Memo:
收稿日期:2018-12-10。
基金项目:国家自然科学基金青年科学基金项目(31802220)
作者简介:张元鹏,助理研究员,博士,主要从事动物传染病防治和免疫技术研究,E-mail:yuanpengzhangnj@163.com。
通信作者:郑其升,研究员,E-mail:immune_tech@126.com。
更新日期/Last Update: 1900-01-01