[1]杨治河,邹彦,曹言,等.铅胁迫对小鼠精子发生和精子活力的影响及机制[J].南京农业大学学报,2019,42(4):752-758.[doi:10.7685/jnau.201903070]
 YANG Zhihe,ZOU Yan,CAO Yan,et al.Lead stress on spermatogenesis and sperm motility in mice impact and mechanism[J].Journal of Nanjing Agricultural University,2019,42(4):752-758.[doi:10.7685/jnau.201903070]
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铅胁迫对小鼠精子发生和精子活力的影响及机制()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年4期
页码:
752-758
栏目:
动物科学
出版日期:
2019-07-08

文章信息/Info

Title:
Lead stress on spermatogenesis and sperm motility in mice impact and mechanism
作者:
杨治河1 邹彦2 曹言2 陈芳慧2 李友余1 陈婧1 王校怡1
1. 滁州城市职业学院解剖学教研室, 安徽 滁州 239000;
2. 南京农业大学动物科技学院, 江苏 南京 210095
Author(s):
YANG Zhihe1 ZOU Yan2 CAO Yan2 CHEN Fanghui2 LI Youyu1 CHEN Jing1 WANG Xiaoyi1
1. Department of Anatomy, Chuzhou City Vocation College, Chuzhou 239000, China;
2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
关键词:
铅胁迫精子发生精液品质基因表达精子活力
Keywords:
lead stressspermatogenesissemen qualitygene expressionsperm motility
分类号:
R321.1
DOI:
10.7685/jnau.201903070
摘要:
[目的]本文旨在探讨铅对小鼠精子发生和精子活力的影响及机制。[方法]建立铅胁迫试验小鼠模型;涂片观察精液品质并拍照;制备精子悬液,用穗加精液分析(SSA)自动检测系统测定精子运动学参数及精子活力参数;用荧光定量PCR检测Oct4、DAZ1、SCP3、Tsc21、ACR、Acrv1、cyclinA1、SPATA46LDH-C4基因的表达水平;观察睾丸组织形态,计数生精小管数和生精细胞数。[结果]与对照组相比,铅胁迫下小鼠活动精子的比例低于50%,精子形态普遍异常;精子曲线运动速度、直线运动速度、平均路径速度、精子头侧摆幅度、鞭打频率、平均角位移与对照组相比均极显著下降(P<0.001),精子运动的直线性、前向性、摆动性与对照组相比无显著差异(P>0.05),精子活力极显著下降(P<0.001);Oct4DAZ1基因表达水平极显著降低(P<0.001),SCP3、ACRAcrv1基因表达水平极显著下降(P<0.01),cyclinA1SPATA46基因表达水平显著降低(P<0.05),而Tsc21LDH-C4基因表达水平无显著差异(P>0.05)。睾丸生精小管和生精细胞数均极显著减少(P<0.001),部分生精细胞坏死,并在生精细胞坏死集中区域可见支持细胞增多,未见成熟的精子,睾丸间质炎性浸润,间质细胞数明显减少。[结论]铅胁迫可引起睾丸间质细胞损伤和生精障碍。铅可能通过下调Oct4、DAZ1、SCP3SPATA46基因表达,使精子数量减少,精子成熟停滞,畸形精子数增加,精子活力下降。
Abstract:
[Objectives]The paper aimed to investigate the effect and mechanism of lead on spermatogenesis and sperm motility in mice.[Methods]The mouse model of lead stress test was established,the semen quality was observed and photographed by smear,the sperm suspension was prepared,and the sperm motility parameters and sperm motility parameters were measured by Suiplus semen analysis(SSA)automatic detection system. The expression levels of Oct4,DAZ1,SCP3,Tsc21,ACR,Acrv1,cyclin A1,SPATA46 and LDH-C4 genes were detected by fluorescence quantitative PCR. The morphology of testicular tissue was observed,the number of seminiferous tubule and spermatogenic cell were counted.[Results]Compared with the control group,the proportion of mobile sperm under lead stress was significantly lower than 50%,and the sperm morphology of mice was generally abnormal. The sperm curvilinear velocity,straight-line velocity,average path velocity,sperm head swing amplitude,whipping frequency and average angular displacement were significantly lower than those in the control group(P<0.001). There was no significant difference in linear,forward and swing between the treatment group and the control group. Sperm motility decreased significantly(P<0.001). Oct4 and DAZ1 gene expression levels significantly decreased(P<0.001),SCP3,ACR and Acrv1 gene expression levels significantly decreased(P<0.001),cyclinA1 and SPATA46 gene expression levels significantly decreased(P<0.05). However,there was no significant difference in gene expression between Tsc21 and LDH-C4. The number of testicular seminiferous tubule and spermatogenic cells decreased significantly(P<0.001). Some spermatogenic cells were necrotic,and sertoli cells increased in the concentrated area of spermatogenic cell necrosis. There was no mature sperm and testicular interstitial inflammatory infiltration. The number of interstitial cells decreased significantly.[Conclusions]Lead stress can cause damage to Leydig cells and sperm dysfunction. Lead may reduce the number of sperm,stagnate sperm maturation,increase the number of abnormal sperm and decrease sperm motility by down-regulating the gene expression of Oct4,DAZ1,SCP3 and SPATA46.

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备注/Memo

备注/Memo:
收稿日期:2019-03-29。
基金项目:滁州城市职业学院自然科学重点项目(2018ZK04)
作者简介:杨治河,副教授,主要从事人体解剖学与组织胚胎学研究,E-mail:yzh6410@aliyun.com。
更新日期/Last Update: 1900-01-01