[1]徐霞,钟秋怡,何彬,等.外源虾青素对分蘖期受淹水稻的补救效应[J].南京农业大学学报,2019,42(6):1006-1013.[doi:10.7685/jnau.201903024]
 XU Xia,ZHONG Qiuyi,HE Bin,et al.Remedial effect of exogenous astaxanthin on submerged rice at tillering stage[J].Journal of Nanjing Agricultural University,2019,42(6):1006-1013.[doi:10.7685/jnau.201903024]
点击复制

外源虾青素对分蘖期受淹水稻的补救效应()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年6期
页码:
1006-1013
栏目:
植物科学
出版日期:
2019-11-15

文章信息/Info

Title:
Remedial effect of exogenous astaxanthin on submerged rice at tillering stage
作者:
徐霞 钟秋怡 何彬 张震 王绍华 丁艳锋 李刚华
南京农业大学江苏省现代作物生产协同创新中心/农业农村部南方作物生理生态重点开放实验室, 江苏 南京 210095
Author(s):
XU Xia ZHONG Qiuyi HE Bin ZHANG Zhen WANG Shaohua DING Yanfeng LI Ganghua
Jiangsu Collaborative Innovation Center for Modern Crop Production/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing 210095, China
关键词:
虾青素(ASTA)水稻分蘖期淹水胁迫抗氧化作用
Keywords:
astaxanthin(ASTA)ricetillering stagesubmergence stressantioxidation
分类号:
S511;S311
DOI:
10.7685/jnau.201903024
摘要:
[目的] 本研究旨在探究淹水胁迫解除后,叶面喷施虾青素(astaxanthin,ASTA)对分蘖期受淹水稻的补救效应。[方法] 以常规粳稻‘宁粳7号’为试验材料,盆栽种植,移栽10 d后进行没顶淹水5 d的处理,淹没结束后设5个处理:正常水分管理(用CT表示);淹水胁迫+解除淹水后喷施清水(CK);淹水胁迫+解除淹水后喷施0.4 mmol·L-1虾青素(ASTA-0.4);淹水胁迫+解除淹水后喷施0.8 mmol·L-1虾青素(ASTA-0.8);淹水胁迫+解除淹水后喷施1.6 mmol·L-1虾青素(ASTA-1.6)。在叶面喷施后0(喷施当天)、1、2、3、5、7 d取样,测定水稻光合色素含量、抗氧化酶[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、谷胱甘肽还原酶(GR)和抗坏血酸过氧化物酶(APX)]活性、丙二醛(MDA)含量、过氧化氢(H2O2)含量和可溶性蛋白含量等指标;在叶面喷施后0(喷施当天)、3、6、9 d取样测定植株干物质量、株高和可溶性总糖含量;在成熟期收获有代表性的3盆测定产量及其构成因素。[结果] 没顶淹水5 d后,与CT相比,水稻茎蘖数和绿叶数显著减少,干物质积累量和叶绿素含量降低,氧化损伤严重,导致产量下降。淹水解除后,与CK相比,叶面喷施虾青素能提高受淹水稻叶片中抗氧化酶活性,增加可溶性总糖和可溶性蛋白含量,降低H2O2和MDA含量,减轻氧化损伤,促进分蘖发生,加快干物质积累,减少产量损失。其中,叶面喷施后12 d,ASTA-0.8处理的茎蘖数、绿叶数以及干物质量、叶绿素a、可溶性总糖和可溶性蛋白含量均明显增加,抗氧化酶活性提高,叶片中H2O2和MDA含量降低;喷施后7 d,ASTA-0.8处理叶片中MDA含量与CK相比显著降低20.7%,H2O2含量降低9.4%;ASTA-0.8处理最终产量为CT产量的98.7%。[结论] 叶面喷施0.8 mmol·L-1虾青素可最大程度补救水稻分蘖期淹水导致的伤害和产量损失。
Abstract:
[Objectives] The study aimed to explore the remedial effect of foliar spraying astaxanthin(ASTA)on submerged rice at tillering stage after de-submergence.[Methods] The rice variety ‘Ningjing 7’ as experimental material was planted in pot,and five treatments were set up at tillering stage:normal moisture management(indicated by CT);submergence stress+spraying water(CK);submergence stress+spraying 0.4 mmol·L-1 ASTA(ASTA-0.4);submergence stress+spraying 0.8 mmol·L-1 ASTA(ASTA-0.8);submergence stress+spraying 1.6 mmol·L-1 ASTA(ASTA-1.6). The leaves of rice were sampled at 0,1,2,3,5,7 days after spraying ASTA,and analyzed on the effect on the photosynthetic pigment content,the activities of antioxidant enzymes(SOD,CAT,POD,APX,GR),membrane lipid peroxidation(MDA)content,hydrogen peroxide(H2O2)content and soluble protein content. The leaves of rice were sampled at 0,3,6,9 days after spraying ASTA,and analyzed on the effect on the dry weight,plant height and total soluble sugar content. The yield and its components were measured by collecting representational three-pot rice at maturity.[Results] The results showed that after five days of submergence the number of tillers and green leaves per plant decreased significantly compared with CT,dry matter accumulation and chlorophyll content decreased,and oxidative damage was serious. Ultimately,it had an impact on yield. After de-submergence,foliar spraying ASTA could increase the activity of antioxidant enzymes,increase the contents of total soluble sugar and soluble protein,reduce the contents of H2O2 and MDA,alleviate oxidative damage,promote tillering,accelerate dry matter accumulation and reduce yield loss. In particularly,at 12 days after spraying,the number of tillers per plant,green leaves,and the contents of dry matter,chlorophyll a,total soluble sugar and soluble protein of ASTA-0.8 treatment increased significantly,and the activities of antioxidant enzymes increased,and the contents of H2O2 and MDA in leaves decreased. At 7 days after spraying,MDA content was 20.7% lower than CK,and H2O2 content was 9.4% lower than CK. The final yield of ASTA-0.8 treatment was the closest to CT,and the yield returned to 98.7% of CT.[Conclusions] When rice was submerged at tillering stage,foliar spraying 0.8 mmol·L-1 astaxanthin could remedy it.

参考文献/References:

[1] 尹朝静.气候变化对中国水稻生产的影响研究[D]. 武汉:华中农业大学,2017. Yin C J. Study on the climate change impact on Chinese rice production[D]. Wuhan:Huazhong Agricultural University,2017(in Chinese with English abstract).
[2] Hirabayashi Y,Mahendran R,Koirala S,et al. Global flood risk under climate change[J]. Nature Climate Change,2013,3(9):816-821.
[3] Jackson B M. Ethylene and responses of plants to soil waterlogging and submergence[J]. Annual Review of Plant Physiology,1985,36(1):145-174.
[4] Armstrong W. Aeration in higher plants[J]. Advances in Botanical Research,1980,7:225-332.
[5] Maurel C,Simonneau T,Sutka M.The significance of roots as hydraulic rheostats[J]. Journal of Experimental Botany,2010,61(12):3191-3198.
[6] Bailey-Serres J,Lee S C,Brinton E.Waterproofing crops:effective flooding survival strategies[J]. Plant Physiology,2012,160(4):1698-1709.
[7] Voesenek L A,Bailey-Serres J. Flood adaptive traits and processes:an overview[J]. The New Phytologist,2015,206(1):57-73.
[8] 田志环. 淹涝胁迫对水稻影响的研究进展[J]. 安徽农业科学,2008,36(1):143-145. Tian Z H. Research advance of the influence of submergence stress on rice[J]. Journal of Anhui Agricultural Sciences,2008,36(1):143-145(in Chinese with English abstract).
[9] Liu M,Chu M J,Ding Y F,et al. Exogenous spermidine alleviates oxidative damage and reduce yield loss in rice submerged at tillering stage[J]. Frontiers in Plant Science,2015,6:919.
[10] Gautam P,Lal B,Raja R,et al. Post-flood nitrogen and basal phosphorus management affects survival,metabolic changes and anti-oxidant enzyme activities of submerged rice(Oryza sativa L.)[J]. Functional Plant Biology,2014,41(12):1284-1294.
[11] Gautam P,Lal B,Tripathi R,et al. Beneficial effects of potassium application in improving submergence tolerance of rice(Oryza sativa L.)[J]. Environmental & Experimental Botany,2016,128:18-30.
[12] Chu M J,Liu M,Ding Y F,et al. Effect of nitrogen and silicon on rice submerged at tillering stage[J]. Agronomy Journal,2017,110(1):183-192.
[13] 姜颖,赵越,孙全军,等. 植物生长调节剂在植物生长发育中的应用[J]. 黑龙江科学,2018,9(24):4-7,11. Jiang Y,Zhao Y,Sun Q J,et al. Application of plant growth regulators on plant growth and development[J]. Heilongjiang Science,2018,9(24):4-7,11(in Chinese with English abstract).
[14] Zhou W,Leul M. Uniconazole-induced alleviation of freezing injury in relation to changes in hormonal balance,enzyme activities and lipid peroxidation in winter rape[J]. Plant Growth Regulation,1998,26(1):41-47.
[15] Hoque M A,Banu M N A,Okuma E,et al. Exogenous proline and glycinebetaine increase NaCl-induced ascorbate-glutathione cycle enzyme activities,and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspension-cultured cells[J]. Journal of Plant Physiology,2007,164(11):1457-1468.
[16] 张健,刘美艳. 一氧化氮提高黄瓜幼苗耐涝性的探究[J]. 中国瓜菜,2017,30(1):18-21. Zhang J,Liu M Y. Enhancement of waterlogging tolerance of cucumber seedlings by nitric oxide[J]. China Cucurbits and Vegetables,2017,30(1):18-21(in Chinese with English abstract).
[17] 姚康飞,张瑞莲,刘晓娟,等. 不同立体构型的虾青素抗脂质过氧化活性研究[J]. 中国食品学报,2018,18(10):86-94. Yao K F,Zhang R L,Liu X J,et al. Studies on anti-lipid peroxidation activity of different stereoisomeric astaxanthin[J]. Journal of Chinese Institute of Food Science and Technology,2018,18(10):86-94(in Chinese with English abstract).
[18] Dose J,Matsugo S,Yokokawa H,et al. Free radical scavenging and cellular antioxidant properties of astaxanthin[J]. International Journal of Molecular Sciences,2016,17(1):103.
[19] 朱丽,钱前. 虾青素功能米:生物强化新思路,优质米培育新资源[J]. 植物学报,2019,54(1):4-8. Zhu L,Qian Q. Astaxanthin functional rice:new idea of biofortification,new perspectives for high-quality rice breeding[J]. Chinese Bulletin of Botany,2019,54(1):4-8(in Chinese with English abstract).
[20] 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000. Li H S. Principles and Techniques of Plant Physiological Biochemical Experiment[M]. Beijing:Higher Education Press,2000(in Chinese).
[21] Jiang M,Zhang J. Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves[J]. Journal of Experimental Botany,2002,53(379):2401-2410.
[22] 王爱国,罗广华. 植物的超氧物自由基与羟胺反应的定量关系[J]. 植物生理学通讯,1990,26(6):55-57. Wang A G,Luo G H. Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants[J]. Plant Physiology Communications,1990,26(6):55-57(in Chinese).
[23] 王学奎. 植物生理生化实验原理和技术[M]. 2版.北京:高等教育出版社,2006. Wang X K. Principles and Techniques of Plant Physiological Biochemical Experiment[M]. 2nd ed. Beijing:Higher Education Press,2006(in Chinese).
[24] Yeung E,van Veen H,Vashisht D,et al. A stress recovery signaling network for enhanced flooding tolerance in Arabidopsis thaliana[J]. Proc Natl Acad Sci USA,2018,115(26):E6085-E6094.
[25] 潘澜,薛立. 植物淹水胁迫的生理学机制研究进展[J]. 生态学杂志,2012,31(10):2662-2672. Pan L,Xue L. Plant physiological mechanisms in adapting to waterlogging stress:a review[J]. Chinese Journal of Ecology,2012,31(10):2662-2672(in Chinese with English abstract).
[26] Mishra A,Mishra K B,H?ermiller I I,et al. Chlorophyll fluorescence emission as a reporter on cold tolerance in Arabidopsis thaliana accessions[J]. Plant Signaling & Behavior,2011,6(2):301-310.
[27] 贾漫丽,李娜,李季生,等. 盐胁迫对4个桑树品种生理生化特性的影响[J]. 西北林学院学报,2016,31(5):96-101. Jia M L,Li N,Li J S,et al. Effects of salt stress on physiological and biochemical characteristics of four mulberry varieties[J]. Journal of Northwest Forestry University,2016,31(5):96-101(in Chinese with English abstract).
[28] Kennedy R A,Rumpho M E,Fox T C. An aerobic metabolism in plants[J]. Plant Physiology,1992,100:16-21.
[29] Singh N K,Bracker C A,Hasegawa P M,et al. Characterization of osmotin:a thaumatin-like protein associated with osmotic adaptation in plant cells[J]. Plant Physiology,1987,85(2):529-536.
[30] 吴潇,陈杨杨,石新杰,等. 喷施外源激素对‘玉露香’梨叶片表皮蜡质组分、结构及渗透性的影响[J]. 南京农业大学学报,2018,41(4):647-654. DOI:10.7685/jnau.201710007. Wu X,Chen Y Y,Shi X J,et al. Effects of spraying exogenous hormones on cuticular wax composition,structure and permeability of the leaves in ‘Yuluxiang’ pear[J]. Journal of Nanjing Agricultural University,2018,41(4):647-654(in Chinese with English abstract).
[31] Jia D J,Fan L M,Shen J L,et al. Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance[J]. Scientia Horticulturae,2019,243:428-433.

相似文献/References:

[1]张辰明,徐烨红,赵海娟,等.不同氮形态对水稻苗期氮素吸收和根系生长的影响[J].南京农业大学学报,2011,34(3):72.[doi:10.7685/j.issn.1000-2030.2011.03.013]
 ZHANG Chen-ming,XU Ye-hong,ZHAO Hai-juan,et al.Effects of different nitrogen forms on nitrogen uptake and root growth of rice at the seedling stage[J].Journal of Nanjing Agricultural University,2011,34(6):72.[doi:10.7685/j.issn.1000-2030.2011.03.013]
[2]郝文雅,沈其荣,冉炜,等.西瓜和水稻根系分泌物中糖和氨基酸对西瓜枯萎病病原菌生长的影响[J].南京农业大学学报,2011,34(3):77.[doi:10.7685/j.issn.1000-2030.2011.03.014]
 HAO Wen-ya,SHEN Qi-rong,RAN Wei,et al.The effects of sugars and amino acids in watermelon and rice root exudates on the growth of Fusarium oxysporum f.sp. niveum[J].Journal of Nanjing Agricultural University,2011,34(6):77.[doi:10.7685/j.issn.1000-2030.2011.03.014]
[3]徐小飒,刘喜,赵志刚,等.培矮64S/93-11重组自交系分子图谱构建及千粒重QTL检测[J].南京农业大学学报,2011,34(1):8.[doi:10.7685/j.issn.1000-2030.2011.01.002]
 XU Xiao-sa,LIU Xi,ZHAO Zhi-gang,et al.Construction of genetic linkage map based on a RILs population derived from the hybrid rice Peiai 64S/93-11 and detection of QTL for 1000-grain weight[J].Journal of Nanjing Agricultural University,2011,34(6):8.[doi:10.7685/j.issn.1000-2030.2011.01.002]
[4]魏广彬,徐海港,丁艳峰,等.水稻设计栽培系统的研制与实现[J].南京农业大学学报,2011,34(1):14.[doi:10.7685/j.issn.1000-2030.2011.01.003]
 WEI Guang-bin,XU Hai-gang,DING Yan-feng,et al.Development and realization of the rice design cultivation system[J].Journal of Nanjing Agricultural University,2011,34(6):14.[doi:10.7685/j.issn.1000-2030.2011.01.003]
[5]李刚华,王惠芝,王绍华,等.穗肥对水稻穗分化期碳氮代谢及颖花数的影响[J].南京农业大学学报,2010,33(1):1.[doi:10.7685/j.issn.1000-2030.2010.01.001]
 LI Gang-hua,WANG Hui-zhi,WANG Shao-hua,et al.Effect of nitrogen applied at rice panicle initiation stage on carbon and nitrogen metabolism and spikelets per panicle[J].Journal of Nanjing Agricultural University,2010,33(6):1.[doi:10.7685/j.issn.1000-2030.2010.01.001]
[6]王碧茜,范晓荣,徐国华,等.不同氮效率水稻品种旗叶的衰老特征[J].南京农业大学学报,2010,33(2):8.[doi:10.7685/j.issn.1000-2030.2010.02.002]
 WANG Bi-qian,FAN Xiao-rong,XU Guo-hua,et al.Characteristics of flag leaf senescence among three rice cultivars with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(6):8.[doi:10.7685/j.issn.1000-2030.2010.02.002]
[7]赵成国,徐海港,李刚华,等.超高产单季粳稻抽穗期群体构成研究[J].南京农业大学学报,2011,34(2):23.[doi:10.7685/j.issn.1000-2030.2011.02.005]
 ZHAO Cheng-guo,XU Hai-gang,LI Gang-hua,et al.Studies on population composition of super-high-yielding single-cropping japonica rice in heading stage[J].Journal of Nanjing Agricultural University,2011,34(6):23.[doi:10.7685/j.issn.1000-2030.2011.02.005]
[8]陈志德,仲维功,王军,等.水稻苗期Cd2+胁迫的QTL定位研究[J].南京农业大学学报,2010,33(3):1.[doi:10.7685/j.issn.1000-2030.2010.03.001]
 CHEN Zhi-de,ZHONG Wei-gong,WANG Jun,et al.Mapping of QTL of tolerance to Cd^{2+} stress at seedling stage in rice(Oryza sativa L.)[J].Journal of Nanjing Agricultural University,2010,33(6):1.[doi:10.7685/j.issn.1000-2030.2010.03.001]
[9]叶利庭,樊剑波,徐晔红,等.不同氮效率水稻的生长特性[J].南京农业大学学报,2010,33(3):77.[doi:10.7685/j.issn.1000-2030.2010.03.015]
 YE Li-ting,FAN Jian-bo,XU Ye-hong,et al.Characteristics of growth in rice genotypes with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(6):77.[doi:10.7685/j.issn.1000-2030.2010.03.015]
[10]晋玉宽,杨世湖,余丽,等.不同启动子驱动下Pib基因的表达及与稻瘟病抗性的关系[J].南京农业大学学报,2010,33(4):1.[doi:10.7685/j.issn.1000-2030.2010.04.001]
 JIN Yu-kuan,YANG Shi-hu,YU Li,et al.Expression and resistance analysis of the Pib gene in transgenic rice under different promoters[J].Journal of Nanjing Agricultural University,2010,33(6):1.[doi:10.7685/j.issn.1000-2030.2010.04.001]

备注/Memo

备注/Memo:
收稿日期:2019-03-15。
基金项目:国家重点研发计划项目(2017YFD0301203,2016YFD0300505,2018YFD0300803);江苏省重点研发计划项目(BE2017369);江苏省自主创新资金项目(CX[18]1002)
作者简介:徐霞,硕士研究生。
通信作者:李刚华,教授,主要从事水稻生理生态研究,E-mail:lgh@njau.edu.cn。
更新日期/Last Update: 1900-01-01