[1]吕娜娜,沈宗专,陶成圆,等.蕉园土壤及香蕉植株不同组织可培养细菌的群落特征[J].南京农业大学学报,2019,42(6):1088-1097.[doi:10.7685/jnau.201902019]
 Lü Nana,SHEN Zongzhuan,TAO Chengyuan,et al.The characteristics of culturable bacterial community in soils and tissue parts of banana[J].Journal of Nanjing Agricultural University,2019,42(6):1088-1097.[doi:10.7685/jnau.201902019]
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蕉园土壤及香蕉植株不同组织可培养细菌的群落特征()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年6期
页码:
1088-1097
栏目:
生物与环境
出版日期:
2019-11-15

文章信息/Info

Title:
The characteristics of culturable bacterial community in soils and tissue parts of banana
作者:
吕娜娜1 沈宗专1 陶成圆1 欧燕楠1 王蓓蓓2 阮云泽2 李荣1 沈其荣1
1. 南京农业大学资源与环境科学学院/江苏省固体有机废弃物资源化研究重点实验室/江苏省有机固体废弃物协同创新中心/国家有机类肥料工程技术研究中心, 江苏 南京 210095;
2. 海南大学热带作物学院, 海南 海口 570228
Author(s):
Lü Nana1 SHEN Zongzhuan1 TAO Chengyuan1 OU Yannan1 WANG Beibei2 RUAN Yunze2 LI Rong1 SHEN Qirong1
1. College of Resources and Enviromental Sciences/Jiangsu Provincial Key Lab of Solid Organic Waste Utilization/Jiangsu Collaborative Innovation Center of Solid Organic Wastes/National Engineering Research Center for Organic-Based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China;
2. College of Tropical Crop Science, Hainan University, Haikou 570228, China
关键词:
香蕉枯萎病抑病型土壤内生菌假单胞菌可培养细菌
Keywords:
banana Fusarium wilt diseasedisease-suppressive soilendophytePseudomonasculturable bacteria
分类号:
S147.5
DOI:
10.7685/jnau.201902019
摘要:
[目的] 本研究旨在比较抑病型与导病型蕉园土体、香蕉根际及不同组织内生可培养细菌群落的组成及功能差异。[方法] 在R2A培养基上分离、筛选,结合16S rDNA测序分析可培养细菌的组成及拮抗尖孢镰刀菌能力。[结果] 分离细菌766株,经鉴定分别属于放线菌门、拟杆菌门、厚壁菌门及变形菌门;蕉园土壤、香蕉根际土壤及不同组织间细菌组成有明显差异,假单胞菌属在抑病型蕉园样品中的平均相对丰度高于导病型蕉园样品,是抑病型蕉园处理区别于导病型蕉园处理最为重要的生防菌。进一步从抑病型蕉园筛选到假单胞菌362株,其数量和多样性均高于导病型蕉园;但导病型蕉园样品中筛选的假单胞菌中具有拮抗尖孢镰刀菌能力的菌株比例高于抑病型蕉园样品。[结论] 蕉园土壤及香蕉不同植株组织是决定可培养微生物群落结构的第一影响因素,土壤抑病能力对其影响不显著。抑病型及导病型蕉园土壤和香蕉不同组织内生菌群落组成有明显差异,尤其是具有生防潜能的假单胞菌属的组成及功能差异明显。
Abstract:
[Objectives] The aim of this study was to explore the differences of composition and function of culturable bacterial community in banana bulk and rhizosphere soils,and culturable endophytes in different tissues planted in Fusarium wilt disease suppressive and conducive orchards.[Methods] Isolation and screening of culturable bacteria on R2A medium and 16S rDNA sequencing were used to identify the culturable bacteria in each sample,and composition of culturable bacteria and their antagonistic ability against pathogenic bacteria were analyzed.[Results] The results showed that in total 766 bacterial strains were isolated and successfully identified,which were affiliated to Actinobacteria,Bacteroidetes,Firmicutes and Proteobacteria. And the composition of isolated bacterial community from bulk soil,rhizosphere soil samples and plant tissues were obviously different. The mean relative abundance of Pseudomonas in samples collected from banana plantation suppressive to Fusarium wilt disease was higher than that in banana plantation conducive to Fusarium wilt disease. Also,this genus played a key role in contribution to community differences. Further isolation and identification of Pseudomonas showed that in total 362 Pseudomonas strains were isolated and successfully identified for all samples collected from banana orchard suppressive to Fusarium wilt disease,which showed a higher abundance and diversity compared to that from banana orchard conducive to Fusarium wilt disease. Moreover,the proportion of Pseudomonas strains antagonistic to Fusarium oxysporum race 4 for all samples collected from banana orchard suppressive to Fusarium wilt disease was higher than that for all samples collected from banana orchard conducive to Fusarium wilt disease.[Conclusions] Soil type and different plant tissues are the first factors affecting the structure of culturable microorganisms. The orchard disease suppression ability is not the significant driver of the difference of culturable bacterial community in the present study,the culturable bacterial community in soils and banana tissues from healthy banana collected from orchard suppressive to Fusarium wilt disease is still different with that from orchard conducive to Fusarium wilt disease. Especially,the composition and function are obvious different in those Pseudomonas,which is identified as potential biocontrol agents.

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备注/Memo

备注/Memo:
收稿日期:2019-02-25。
基金项目:国家重点研发计划项目(2017YFD0202101);中央高校基本科研业务费专项资金(KJQN201746);国家自然科学基金项目(31601836);中国博士后科学基金项目(2016M590469,2018T110509)
作者简介:吕娜娜,硕士研究生。
通信作者:李荣,教授,博导,主要从事土壤微生物与生物肥料研究,E-mail:lirong@njau.edu.cn。
更新日期/Last Update: 1900-01-01