[1]李志琳,解宇峰,吴杰,等.一株高效柴油降解菌Serratia sp.J-3的筛选、鉴定和降解特性[J].南京农业大学学报,2019,42(6):1098-1107.[doi:10.7685/jnau.201901050]
 LI Zhilin,XIE Yufeng,WU Jie,et al.Isolation and identification of a high efficiency diesel oil degrading strain Serratia sp. J-3 and its degradation characteristics[J].Journal of Nanjing Agricultural University,2019,42(6):1098-1107.[doi:10.7685/jnau.201901050]
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一株高效柴油降解菌Serratia sp.J-3的筛选、鉴定和降解特性()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年6期
页码:
1098-1107
栏目:
生物与环境
出版日期:
2019-11-15

文章信息/Info

Title:
Isolation and identification of a high efficiency diesel oil degrading strain Serratia sp. J-3 and its degradation characteristics
作者:
李志琳1 解宇峰2 吴杰1 徐佳迎1 王珏1 蒋静艳1
1. 南京农业大学资源与环境科学学院, 江苏 南京 210095;
2. 生态环境部南京环境科学研究所, 江苏 南京 210042
Author(s):
LI Zhilin1 XIE Yufeng2 WU Jie1 XU Jiaying1 WANG Jue1 JIANG Jingyan1
1. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;
2. Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
关键词:
沙雷氏菌柴油生长特性alkBalmA生物降解
Keywords:
Serratiadieselgrowth characteristicsalkBalmAbiodegradation
分类号:
X172
DOI:
10.7685/jnau.201901050
摘要:
[目的] 本文旨在筛选出不同种类和特性的高效降解柴油菌株,研究降解菌株对不同环境下柴油污染的降解机制。[方法] 从南京扬子石化厂区的油泥沉积物中分离出1株高效柴油降解菌J-3,通过细菌形态学、生理生化及16S rRNA基因序列分析比对初步鉴定菌株J-3为沙雷氏菌属(Serratia sp.)。通过水培及土壤培养并结合气质联用(GC-MS)和全基因组比对技术研究其降解率和降解机制。[结果] 该菌能利用柴油为唯一碳源生长,产生表面活性物质,具有稳定的乳化性能。在温度20~40℃、初始pH5~9条件下生长良好,能耐受15 g·L-1 NaCl和5 g·L-1柴油。在最适生长条件(pH7,25℃,1 g·L-1 NaCl,0.5 g·L-1柴油)下对柴油降解率为62.0%,GC-MS图谱分析确定菌株J-3对柴油中各组分均有降解能力,对部分中长直链烷烃降解率超过99%。通过菌株J-3的全基因组比对分析,发现2个烷烃氧化相关的基因,烷烃羟化酶基因alkB和长链烷烃羟化酶基因almA,推断菌株J-3能降解柴油可能是这2个氧化酶基因产生作用。将菌株J-3应用于柴油污染土壤修复试验,对土壤中各柴油组分均有降解能力。不同处理下菌株J-3降解率可达33.8%~49.0%,可有效降解土壤中的柴油。[结论] Serratia sp.J-3是一株高效降解柴油的菌株,在水体和土壤中均能很好利用柴油进行生长代谢,可应用于柴油污染实际生物修复工程。
Abstract:
[Objectives] The paper aimed to screen different kinds and properties of diesel strains with high degradation capacity and study their degrading mechanism in different environmental media.[Methods] A high-efficiency diesel degrading bacteria named as strain J-3 was isolated from the sludge sediments of Nanjing Yangzi Petrochemical Plant. The physiological and biochemical test together with 16S rRNA gene sequencing analysis indicated that the strain J-3 belonged to Serratia sp.. The degradation efficiency of diesel and the degradation mechanism were studied by hydroponic and soil culture methods combined with gas chromatography-mass spectrometric analysis(GC-MS)and genome comparison techniques.[Results] The strain could efficiently grow using diesel as its sole carbon and energy source. It could produce surfactant,which showed it had a stable emulsifying property. The strain grew well under the temperature of 20-40℃,and the pH of 5-9 conditions,it could tolerate 15 g·L-1 salinity and 5 g·L-1 diesel concentration. The degradation efficiency of diesel under the optimal growth conditions(pH7,25℃,1 g·L-1 salinity and 0.5 g·L-1 diesel concentration)was 62.0%. GC-MS spectrum analysis confirmed that J-3 had the ability to degrade all components in diesel oil,and the degradation of some media and long straight-chain alkanes exceeded 99%. Based on the whole-genome DNA sequence analysis of the strain J-3,the two genes associated with alkane oxidation were found,namely alkB,an alkane hydroxylase gene,and almA,a long-chain alkane monooxygenase gene. These two oxidase genes might play a role in the degradation of diesel oil by strain J-3. In addition,when the strain J-3 was applied to the soil remediation test of diesel pollution,it could degrade all diesel components in the soil. The degradation of strain J-3 under different treatments was up to 33.8%-49.0%,which could effectively degrade diesel in soil.[Conclusions] The strain J-3 was a high-efficiency diesel degradation bacterial strain. It can be well used for growth in both water and soil,and can be applied to the actual bioremediation project of diesel oil pollution.

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备注/Memo

备注/Memo:
收稿日期:2019-01-31。
基金项目:国家自然科学基金项目(41675148)
作者简介:李志琳,硕士研究生。
通信作者:蒋静艳,副教授,主要研究方向为环境污染与全球变化,E-mail:lilacjjy@njau.edu.cn。
更新日期/Last Update: 1900-01-01