[1]刘朋,徐宏军,王麒文,等.口服表达猪流行性腹泻病毒S蛋白的重组枯草芽胞杆菌对母猪乳源性免疫力的影响[J].南京农业大学学报,2019,42(6):1118-1123.[doi:10.7685/jnau.201902002]
 LIU Peng,XU Hongjun,WANG Qiwen,et al.Effect on lactogenic immunity of sows by orally recombinant Bacillus subtilis expressing the spike protein of porcine epidemic diarrhea virus[J].Journal of Nanjing Agricultural University,2019,42(6):1118-1123.[doi:10.7685/jnau.201902002]
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口服表达猪流行性腹泻病毒S蛋白的重组枯草芽胞杆菌对母猪乳源性免疫力的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年6期
页码:
1118-1123
栏目:
动物科学
出版日期:
2019-11-15

文章信息/Info

Title:
Effect on lactogenic immunity of sows by orally recombinant Bacillus subtilis expressing the spike protein of porcine epidemic diarrhea virus
作者:
刘朋1 徐宏军2 王麒文2 王永恒1 董婷婷2 杨倩1
1. 南京农业大学动物医学院, 江苏 南京 210095;
2. 青岛蔚蓝生物制品有限公司, 山东 青岛 266114
Author(s):
LIU Peng1 XU Hongjun2 WANG Qiwen2 WANG Yongheng1 DONG Tingting2 YANG Qian1
1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
2. Qingdao Weilan Biological Products Co. Ltd., Qingdao 266114, China
关键词:
猪流行性腹泻病毒S蛋白重组枯草芽胞杆菌乳源性免疫力特异性SIgA中和抗体INF-γTGF-α
Keywords:
porcine epidemic diarrhea virusS proteinrecombinant Bacillus subtilislactogenic immunityspecific SIgAneutralizing antibodyINF-γTGF-α
分类号:
S852.4
DOI:
10.7685/jnau.201902002
摘要:
[目的] 通过给母猪口服表达猪流行性腹泻病毒(PEDV) S蛋白的重组枯草芽胞杆菌以评估其产生的乳源性免疫力,从而为仔猪提供相应的保护。[方法] 分别给母猪口服2 mL重组枯草芽胞杆菌(1×109 CFU·mL-1)和肌肉注射2 mL灭活PEDV疫苗(1×107 TCID50·mL-1),产后1 d收集相应的样品。首先检测了乳汁中特异性SIgA及乳汁细胞中特异性IgA,然后对口腔和鼻腔分泌物中特异性SIgA和细胞因子(INF-γ和TGF-α)的水平进行了检测;最后评估乳汁中的中和抗体滴度。[结果] 口服重组枯草芽胞杆菌后乳汁中SIgA及其乳汁细胞中IgA特异性抗体水平显著提高(P<0.05,P<0.01),同时乳汁及其乳汁细胞中INF-γ和TGF-α的水平也显著提高(P<0.05,P<0.01);鼻腔和口腔分泌物中特异性SIgA显著增加(P<0.05,P<0.01);口服重组枯草芽胞杆菌的母猪乳汁中和抗体滴度为1:128,而肌注组母猪为1:64。[结论] 口服表达PEDV S蛋白的重组枯草芽胞杆菌可提高母猪乳汁中体液免疫和细胞免疫水平,为预防新生仔猪PED的发生提供充足的乳源性免疫力。
Abstract:
An effective strategy to prevent porcine epidemic diarrhea(PED)is to transfer immunity to newborn piglets by milk after oral immunization of sows. The secretory IgA(SIgA)and cytokines of milk play an important roles in the lactogenic immunity of sows.[Objectives] In this study,sows were taken orally recombinant Bacillus subtilis expressing S protein to evaluate the lactogenic immunity,so as to provide corresponding protection for piglets.[Methods] Sows were taken orally 2 mL recombinant Bacillus subtilis(1×109 CFU·mL-1)and injected intramuscularly 2 mL inactivated porcine epidemic diarrhea virus(PEDV)vaccine(1×107 TCID50·mL-1). Corresponding samples were collected at one day after delivery. Collecting milk and cells in it were used to test specific secretory IgA(SIgA),and then the levels of specific SIgA and cytokines(INF-γ and TGF-α)in oral and nasal secretions were detected;finally,the titer of neutralizing antibody in milk was evaluated.[Results] The levels of specific SIgA and IgA antibodys and the INF-γ and TGF-α in the milk and cells significantly increased(P<0.05,P<0.01). There was also a significant increase in the nasal and oral secretions(P<0.05,P<0.01). The titer of neutralizing antibody in the milk of sows with oral administration of recombinant Bacillus subtilis was 1:128,while sows with intramuscular injection was 1:64.[Conclusions] Recombinant Bacillus subtilis that expresses PEDV S protein orally can improve the humoral and cellular immune levels in sows’ milk,providing adequate lactogenic immunity for the prevention of PED in newborn pigs.

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备注/Memo

备注/Memo:
收稿日期:2019-02-14。
基金项目:国家自然科学基金重点项目(31930109)
作者简介:刘朋,博士研究生。
通信作者:杨倩,教授,博导,研究方向动物黏膜免疫,E-mail:zxbyq@njau.edu.cn。
更新日期/Last Update: 1900-01-01