WANG Bin,LIU Junze,CHEN Zhijie,et al.Protective effects of sugar cane extract against oxidative damage induced by diquat and mechanism mediated by intestinal microbiota in weaned piglets[J].Journal of Nanjing Agricultural University,2020,43(3):514-522.[doi:10.7685/jnau.201906025]





Protective effects of sugar cane extract against oxidative damage induced by diquat and mechanism mediated by intestinal microbiota in weaned piglets
王彬12 刘俊泽1 陈志杰2 黄凯1 李延森1 李春梅1
1. 南京农业大学动物科技学院, 江苏 南京 210095;
2. 江苏食品药品职业技术学院, 江苏 淮安 223005
WANG Bin12 LIU Junze1 CHEN Zhijie2 HUANG Kai1 LI Yansen1 LI Chunmei1
1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2. Jiangsu Food&Pharmaceutical Science College, Huai’an 223005, China
sugar cane extractoxidative damagediquatintestinal microbiotaweaned piglets
[目的]本试验旨在探究甘蔗提取物(SCE)对敌草快诱导断奶仔猪氧化损伤的防护效果及其肠道微生物的参与机制。[方法]选择28头断奶仔猪,随机分为4组:对照(Cont)组、敌草快(Diqu)组、1%(质量分数)甘蔗提取物添加(SCE1)组和2%甘蔗提取物添加(SCE2)组。后3组在第21天早晨空腹腹腔注射10 mg·kg-1敌草快溶液,对照组注射等剂量的生理盐水,于第24天屠宰,取血样测定氧化应激指标(谷胱甘肽过氧化物酶、总抗氧化能力、丙二醛、二胺氧化酶和超氧化物岐化酶)和免疫球蛋白,取结肠食靡样品测定并分析细菌群落。[结果]注射敌草快后72 h内,Diqu组和SCE1组仔猪体质量呈现负增长,添加2% SCE显著提高断奶仔猪平均日增重和平均日采食量(P<0.05),显著改善饲料转化效率(P<0.05)。同时提高血液免疫球蛋白IgM水平和谷胱甘肽过氧化酶活性。在肠道微生物方面,4个组间的结肠微生物β多样性具有显著差异。在门水平上,SCE2组结肠中的螺旋菌门、广古菌门和疣微菌门的相对丰度显著低于Diqu组(P<0.05)。在属和OTU的水平上,添加2% SCE显著增加潜在有益菌LactobacillusRuminococcus gauvreauii group的相对丰度,降低潜在有害菌Clostridium sensu stricto 1、KlebsiellaSharpea的相对丰度。[结论]日粮添加2% SCE可在氧化应激条件下保护仔猪肠道微生物物种多样性,缓解敌草快造成的氧化损伤。
[Objectives] The objectives of the study were to evaluate the effects of sugar cane extract(SCE)on alleviating gut oxidative stress induced by diquat and underline the mechanisms mediated by intestinal microbiota in weaned piglets. [Methods] Twenty-eight weaned piglets were randomly divided into four groups;the control group(Cont),diquat group(Diqu),1% SCE supplementation group(SCE1)and 2% SCE supplementation group(SCE2). On 21 d,piglets in the last three groups were intraperitoneally injected with 10 mg·kg-1 of Diqu solution,and piglets in the Cont group were intraperitoneally injected with physical saline. All piglets were killed on 24 d. The blood samples were taken to measure the glutathione peroxidase(GSH-PX),total antioxidant capacity(T-AOC),malondialdehyde(MDA),diamine oxidase(DAO)and superoxide dismutase(SOD),together with IgA,IgG and IgM. The colonic digesta were taken for microbial diversity analysis. [Results] During the 72 hours after diquat injection,the piglet’s body weight was negatively growing in Diqu and SCE1 groups,while the average daily gain and average daily feed intake increased in SCE2 group(P<0.05),and also feed conversion efficiency in piglets improved(P<0.05). Meanwhile,SCE supplementation increased the concentration of IgM and GSH-PX in the blood(P<0.05). Concurrently,a significant discrepancy in colonic microbiota existed among four groups. At the phylum level,when compared to the Diqu group,2% SCE supplementation decreased the relative abundance of Spirochaetae,Euryarchaeota and Verrucomicrobia(P<0.05). At the genus level,increases in relative abundances of Lactobacillus and Ruminococcus gauvreauii group,but the decreases in relative abundances of Clostridium sensu stricto 1,Klebsiella and Sharpea were observed in the SCE supplementation group. [Conclusions] Dietary supplementation with 2% SCE might alleviate the gut oxidative stress induced by Diqu,through promoting colonization of beneficial bacteria,and inhibiting colonization of harmful pathogens in the colon of weaned piglets.


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更新日期/Last Update: 1900-01-01