[1]俞雯雯,袁炳钦,冯汝晴,等.嘴突凸脐蠕孢菌作为微生物除草剂的潜力研究[J].南京农业大学学报,2020,43(4):613-620.[doi:10.7685/jnau.201910018]
 YU Wenwen,YUAN Bingqin,FENG Ruqing,et al.Study on the potential of Exserohilum rostratum as a microbial herbicide[J].Journal of Nanjing Agricultural University,2020,43(4):613-620.[doi:10.7685/jnau.201910018]
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嘴突凸脐蠕孢菌作为微生物除草剂的潜力研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
43卷
期数:
2020年4期
页码:
613-620
栏目:
植物科学
出版日期:
2020-07-13

文章信息/Info

Title:
Study on the potential of Exserohilum rostratum as a microbial herbicide
作者:
俞雯雯 袁炳钦 冯汝晴 郑镇桂 刘灵锐 陈勇
华南农业大学农学院杂草研究实验室, 广东 广州 510642
Author(s):
YU Wenwen YUAN Bingqin FENG Ruqing ZHENG Zhengui LIU Lingrui CHEN Yong
Weed Research Laboratory of Agricultural College, South China Agricultural University, Guangzhou 510642, China
关键词:
嘴突凸脐蠕孢菌最佳培养条件产孢量孢子萌发农药相容性
Keywords:
Exserohilum rostratumoptimum culture conditionspore-producingspore germinationherbicides compatibility
分类号:
S435
DOI:
10.7685/jnau.201910018
摘要:
[目的] 本研究旨在探究嘴突凸脐蠕孢菌(Exserohilum rostratum)的生物学特性及其孢子悬浮液与常用农药混配后孢子萌发特性,为该菌株的生产和应用开发奠定基础。[方法] 以菌株Y9511为试验材料,通过配制完全培养基及缺少碳源、氮源、磷、硫、钾、铁、镁等元素的培养基,用葡萄糖、麦芽糖、可溶性淀粉、α-乳糖分别更换完全培养基的碳源蔗糖,用Ca(NO32、(NH42SO4、大豆蛋白胨分别更换完全培养基的氮源NH4NO3,探究培养基对Y9511的菌丝生长及产孢量的影响。在5~40℃、24 h光照/24 h黑暗、pH3~9的培养条件下探究Y9511的产孢量及孢子萌发率。Y9511与丙草胺、恶唑酰草胺、氰氟草酯、莠灭净、春雷霉素、苯甲嘧菌酯、多菌灵、苯甲丙环唑等8种农药分别混配,探究Y9511和不同农药混配后的孢子萌发率。[结果] 菌株Y9511生长需要碳、磷、硫3种元素,Y9511生长受到氮、铁的抑制,微量元素铜和锌抑制Y9511的生长。缺钾和缺铜的培养基上Y9511基本不产孢,缺硫和缺锌的培养基产孢量较完全培养基高,为3.76×106 mL-1;5种碳源中,以蔗糖为碳源的培养基产孢量最大,为1.47×106 mL-1,4种氮源中,NH4NO3为氮源的培养基产孢量最大,为1.47×106 mL-1;Y9511在低于15℃和高于35℃的条件下产孢受到抑制,32℃时产孢量达到最大值,为8.15×106 mL-1;pH值低于3.02和高于9.50时,产孢量较低,pH值为6.24时,产孢量达到最大值,为2.77×106 mL-1。30℃时孢子萌发率最高,达98.5%;pH值为6.24时,孢子萌发率达到最大值,为85.8%。8种农药对孢子萌发均具有抑制作用,抑制率为5.0%~89.4%。Y9511与供试农药氰氟草酯的相容性最好,3种处理浓度下抑制率最高仅为7%;其次是多菌灵,3种浓度下抑制率为7.8%~19.5%;相容性最差的是苯甲嘧菌酯,其抑制率为25.1%~85.4%。[结论] 嘴突凸脐蠕孢菌生长的最适温度为32℃,pH值为6,钾和锌为菌株培养的必需元素,最适碳源为蔗糖,氮源为硝酸铵;孢子萌发最适温度为30℃,最佳pH值为6~7,与农药氰氟草酯混用后孢子萌发基本不受抑制。
Abstract:
[Objectives] The purpose of this study was to explore the optimal culture conditions of Exserohilum rostratum Y9511 and the spore germination of the E.rostratum combination with agricultural chemicals.[Methods] Strain Y9511 was used as experimental material,and the complete medium and the medium lacking carbon,nitrogen,phosphorus,sulfur,potassium,iron and magnesium were prepared. To explore the effect of the carbon source and nitrogen source on the mycelial growth and sporulation of the strain,we used the glucose,maltose,soluble starch and α-lactose to replace carbon source of the complete culture medium and used the Ca(NO3)2,(NH4)2SO4,soybean peptone to replace nitrogen source of the complete culture medium,respectively. The sporulation and spore germination rate of the strain were explored under the conditions of 5-40℃,24 h light/24 h dark and pH3-9,respectively. To explore the spore germination rate of the strain,we mixed strains with eight pesticides,such as propionamide,oxazolyl oxalamide,cyanofluoroacetate,atrazine,chunleimycin,benazoxystrobin,carbendazim and benzopropionazole,respectively.[Results] The growth of the strain needed three elements:C,P and S,inhibited by N,Fe and trace elements copper,zinc. The spore production of the strain on the medium lacking K and Cu was basically non-sporulation,and the spore production of the medium lacking S and Zn was higher than that of the complete medium,which was 3.76×106 mL-1. Among the five carbon sources,the spore production of the medium using sucrose as the carbon source was the largest,which was 1.47×106 mL-1. Among the four nitrogen sources,NH4NO3 as the nitrogen source produced the largest amount of spores,which was 1.47×106 mL-1. The strain Y9511 was inhibited under the conditions of less than 15℃ and above 35℃,and the spore production reached the maximum value 8.15×106 mL-1 at 32℃. When the pH was lower than 3.02 and higher than 9.50,the sporulation amount was lower,and when the pH value was 6.24,the sporulation amount reached the maximum value of 2.77×106 mL-1. At 30℃,the spore germination rate was the highest,reaching 98.5%;when the pH value was 6.24,the highest germination rate was 85.8%. And eight kinds of herbicides had inhibitory effect on conidia germination,the range of inhibition rate was 5.0%-89.4%,strain Y9511 had the best compatibility with the cyhalofop-butyl,and the highest inhibition rate was only 7% under the three concentrations;the second was carbendazim,and the inhibition rate range was 7.8%-19.5% under the three concentrations,and the worst compatibility was benzoxystrobin,with the inhibition rate ranging from 25.1% to 85.4%.[Conclusions] The optimum temperature for growth of E.rostratum is 32℃,pH value is 6,medium C,P,S are essential elements,the most suitable carbon source is sucrose,and the nitrogen source is ammonium nitrate;the optimal temperature for conidial germination is 30℃,the optimal pH value is 6-7,and spore germination rate is not substantially inhibited mixed with the cyhalofop-methyl.

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备注/Memo

备注/Memo:
收稿日期:2019-10-17。
基金项目:国家重点研发计划项目(2017YFD0201300);国家自然科学基金项目(31672039)
作者简介:俞雯雯,硕士研究生。
通信作者:陈勇,教授,研究方向为杂草生物学与防治,E-mail:chenyong@scau.edu.cn。
更新日期/Last Update: 1900-01-01