LIU Mingni,HU Yun,FANG Xi,et al.Effect of chronic corticosterone exposure on growth performance and hepatic iron homeostasis in broilers[J].Journal of Nanjing Agricultural University,2020,43(4):714-719.[doi:10.7685/jnau.201907008]





Effect of chronic corticosterone exposure on growth performance and hepatic iron homeostasis in broilers
刘明妮 胡云 方茜 马文强 赵茹茜
南京农业大学农业农村部动物生理生化重点实验室/动物医学院, 江苏 南京 210095
LIU Mingni HU Yun FANG Xi MA Wenqiang ZHAO Ruqian
Key Laboratory of Animal Physiological and Biochemistry, Ministry of Agriculture and Rural Affairs/College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
corticosteronebroilerliveriron metabolism
[目的] 本文旨在研究慢性皮质酮处理后肉鸡生长性能及肝脏铁稳态的变化。[方法] 选取30只49日龄如皋黄羽雄性肉鸡,随机均分为对照组和皮质酮组。肉鸡均饲喂玉米豆粕型日粮,对照组肉鸡注射15%(体积分数)乙醇4.0 mg·kg-1,皮质酮组肉鸡注射4.0 mg·kg-1皮质酮,连续注射7 d。记录试验起始及结束时肉鸡体质量,检测肉鸡血清及组织铁代谢指标、十二指肠和肝脏铁代谢相关基因及蛋白的表达。[结果] 与对照组相比,皮质酮暴露极显著抑制肉鸡生长(P<0.01),且极显著增加肉鸡血清铁含量,提高总铁结合力、不饱和铁结合力和转铁蛋白饱和度(P<0.01)及上调肝脏转铁蛋白(TF)表达(P<0.01),但降低肝脏铁含量(P<0.01)及肝脏转铁蛋白受体1(TFR1)蛋白的表达(P=0.06)。[结论] 慢性皮质酮暴露可降低肉鸡生长性能,增加血清铁水平和总铁结合力,减少肝脏铁沉积,并且此暴露对肝脏铁稳态的调节与TF高表达和TFR1低表达密切相关。
[Objectives] The purpose of this papers was to study the effect of chronic corticosterone exposure on growth performance and liver homeostasis in broilers.[Methods] Thirty 49 day-old broilers were randomly divided into control group and corticosterone group. The broilers were fed the same diet. The broilers of control group were injected with 15%(volume traction) alcohol 0.4 mg·kg-1,and corticosterone group broilers were injected with 4.0 mg·kg-1 corticosterone for 7 consecutive days. Body weight was recorded at the beginning and end of the experiment. The serum and tissue iron metabolism parameters,duodenum and liver iron metabolism-related genes and proteins expression were detected.[Results] Compared with the control,corticosterone exposure significantly inhibited broilers growth(P<0.01),increased serum iron,total iron binding capacity,unsaturated iron binding capacity and transferrin saturation(P<0.01) and hepatic transferrin(TF) expression(P<0.01),but greatly decreased hepatic iron content(P<0.01) and liver transferrin receptor 1(TFR1) protein expression(P=0.06).[Conclusions] Corticosterone exposure inhibited broilers growth,increased serum iron levels and total iron binding capacity,and reduced hepatic iron deposition. The hepatic iron homeostasis induced by chronic corticosterone exposure was closely related to high TF expression and low expression of TFR1.


[1] Yal?in S,Ozkan S,Türkmut L,et al. Responses to heat stress in commercial and local broiler stocks.2.Developmental stability of bilateral traits[J]. British Poultry Science,2001,42(2):153-160.
[2] Deeb N,Cahaner A. Genotype-by-environment interaction with broiler genotypes differing in growth rate. 3.Growth rate and water consumption of broiler progeny from weight-selected versus nonselected parents under normal and high ambient temperatures[J]. Poultry Science,2002,81(3):293-301.
[3] Deng W,Dong X F,Tong J M,et al. The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production,gut morphology and intestinal mucosal immunity in laying hens[J]. Poultry Science,2012,91(3):575-582.
[4] Song J,Xiao K,Ke Y L,et al. Effect of a probiotic mixture on intestinal microflora,morphology,and barrier integrity of broilers subjected to heat stress[J]. Poultry Science,2014,93(3):581-588.
[5] 王松波,邓琳,赵婕,等. 热应激对肉鸡抗氧化能力及腓肠肌纤维类型的影响[J]. 华南农业大学学报,2015,36(6):23-28. Wang S B,Deng L,Zhao J,et al. Effects of heat stress on antioxidant capacity and gastrocnemius fiber types in broilers[J]. Journal of South China Agricultural University,2015,36(6):23-28(in Chinese with English abstract).
[6] 钟光,施寿荣,邵丹,等. 持续热应激对黄羽肉鸡生长性能、肉品质和血液指标的影响[J]. 动物营养学报,2018,30(10):3923-3929. Zhong G,Shi S R,Shao D,et al. Effects of continuous heat stress on growth performance,meat quality and blood parameters of yellow-feathered broilers[J]. Chinese Journal of Animal Nutrition,2018,30(10):3923-3929(in Chinese with English abstract).
[7] 李叶涵,周佳,张越,等. L-精氨酸和α-酮戊二酸对热应激肉鸡肝脏功能的影响[J]. 饲料工业,2016,37(16):6-11. Li Y H,Zhou J,Zhang Y,et al. Effects of L-arginine and α-ketoglutaric acid on liver function in heat-stressed broilers[J]. Feed Industry,2016,37(16):6-11(in Chinese with English abstract).
[8] 张少帅,甄龙,冯京海,等. 持续偏热处理对肉仔鸡免疫器官指数、小肠形态结构和黏膜免疫指标的影响[J]. 动物营养学报,2015,27(12):3887-3894. Zhang S S,Zhen L,Feng J H,et al. Effects of continuous partial heat treatment on immune organ index,intestinal morphological structure and mucosal immune index in broilers[J]. Chinese Journal of Animal Nutrition,2015,27(12):3887-3894(in Chinese with English abstract).
[9] Dozier W A Ⅲ,Thaxton J P,Purswell J L,et al. Stocking density effects on male broilers grown to 1.8 kilograms of body weight[J]. Poultry Science,2006,85(2):344-351.
[10] 卢营杰,苗志强,李建慧,等. 饲养密度与活动空间对肉鸡免疫和应激指标的影响[J]. 动物营养学报,2016,28(9):2927-2935. Lu Y J,Miao Z Q,Li J H,et al. Effects of stocking density and activity space on immune and stress indicators in broilers[J]. Chinese Journal of Animal Nutrition,2016,28(9):2927-2935(in Chinese with English abstract).
[11] 常双双,柳青秀,张敏红,等. 饲养密度对肉鸡盲肠菌群多样性、挥发性脂肪酸和血清脑肠肽的影响[J]. 动物营养学报,2018,30(3):938-946. Chang S S,Liu Q X,Zhang M H,et al. Effects of stocking density on cecal flora diversity,volatile fatty acids and serum brain gut peptide in broilers[J]. Chinese Journal of Animal Nutrition,2018,30(3):938-946(in Chinese with English abstract).
[12] Conrad M E,Umbreit J N,Moore E G,et al. Iron absorption and transport[J]. The American Journal of the Medical Sciences,1999,318(4):213-229.
[13] Hentze M W,Muckenthaler M U,Galy B,et al. Two to tango:regulation of mammalian iron metabolism[J]. Cell,2010,142(1):24-38.
[14] Kramer J H,Spurney C F,Iantorno M,et al. d-propranolal protects against oxidative stress and progressive cardiac dysfunction in iron overloaded rats[J]. Canadian Journal of Physiology and Pharmacology,2012,90(9):1257-1268.
[15] Zhao M,Chen J B,Wang W Y,et al. Psychological stress induces hypoferremia through the IL-6-hepcidin axis in rats[J]. Biochemical and Biophysical Research Communications,2008,373(1):90-93.
[16] Chen J B,Shen H,Chen C J,et al. The effect of psychological stress on iron absorption in rats[J]. BMC Gastroenterology,2009,9(1):83.
[17] He F,Ma L,Wang H Y,et al. Glucocorticoid causes iron accumulation in liver by up-regulating expression of iron regulatory protein 1 gene through GR and STAT5[J]. Cell Biochemistry and Biophysics,2011,61(1):65-71.
[18] 滕文锋,施丽飞,侯殿东,等. 慢性束缚应激对小鼠全血铁锌钙镁含量的影响[J]. 现代预防医学,2007,34(7):1201-1203. Teng W F,Shi L F,Hou D D,et al. Effect of chronic restraint stress on the content of iron,zinc,calcium and magnesium in whole blood of mice[J]. Modern Preventive Medicine,2007,34(7):1201-1203(in Chinese with English abstract).
[19] Harsini S G,Habibiyan M,Moeini M M,et al. Effects of dietary selenium,vitamin E,and their combination on growth,serum metabolites,and antioxidant defense system in skeletal muscle of broilers under heat stress[J]. Biological Trace Element Research,2012,148(3):322-330.
[20] 邓发清. 热应激对泌乳奶牛抗氧化性能及微量元素代谢的影响[J]. 中国畜牧杂志,2008,44(5):61-63. Deng F Q. Effects of heat stress on antioxidant activity and trace element metabolism in lactating dairy cows[J]. Chinese Journal of Animal Science,2008,44(5):61-63(in Chinese with English abstract).
[21] Kamel N N,Ahmed A M H,Mehaisen G M K,et al. Depression of leukocyte protein synthesis,immune function and growth performance induced by high environmental temperature in broiler chickens[J]. International Journal of Biometeorology,2017,61(9):1637-1645.
[22] 高志参,李照耀,周静,等. 4个种属Mx蛋白抑制水疱性口炎病毒的研究[J]. 南京农业大学学报,2017,40(4):690-696. DOI:10.7685/jnau.201703023. Gao Z C,Li Z Y,Zhou J,et al. Research of Mx proteins from four species against vesicular stomatitis virus[J]. Journal of Nanjing Agricultural University,2017,40(4):690-696(in Chinese with English abstract).
[23] 苏红光,张敏红,冯京海,等. 持续冷热环境对肉鸡生产性能、糖代谢和解偶联蛋白mRNA表达的影响[J]. 动物营养学报,2014,26(11):3276-3283. Su H G,Zhang M H,Feng J H,et al. Effects of continuous hot and cold environment on production performance,glucose metabolism and uncoupling protein mRNA expression in broilers[J]. Chinese Journal of Animal Nutrition,2014,26(11):3276-3283(in Chinese with English abstract).
[24] Pesti G M,Howarth B. Effects of population density on the growth,organ weights,and plasma corticosterone of young broiler chicks[J]. Poultry Science,1983,62(6):1080-1083.
[25] 罗梦圆,严思思,李晓文,等. 复合免疫应激对肉鸡生产性能的影响[J]. 中兽医医药杂志,2017,36(1):34-36. Luo M Y,Yan S S,Li X W,et al. Effects of compound immune stress on performance of broilers[J]. Journal of Traditional Chinese Veterinary Medicine,2017,36(1):34-36(in Chinese with English abstract).
[26] Bartov I. Corticosterone and fat deposition in broiler chicks:effect of injection time,breed,sex and age[J]. British Poultry Science,1982,23(2):161-170.
[27] Bartov I. Effects of dietary protein concentration and corticosterone injections on energy and nitrogen balances and fat deposition in broiler chicks[J]. British Poultry Science,1985,26(3):311-324.
[28] Shini S,Shini A,Huff G R. Effects of chronic and repeated corticosterone administration in rearing chickens on physiology,the onset of lay and egg production of hens[J]. Physiology & Behavior,2009,98(1/2):73-77.
[29] Jahng J W,Kim N Y,Ryu V,et al. Dexamethasone reduces food intake,weight gain and the hypothalamic 5-HT concentration and increases plasma leptin in rats[J]. European Journal of Pharmacology,2008,581(1/2):64-70.
[30] Wang S,Ni Y D,Guo F,et al. Effect of corticosterone on growth and welfare of broiler chickens showing long or short tonic immobility[J]. Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2013,164(3):537-543.
[31] Ahmed A A,Ma W Q,Ni Y D,et al. Corticosterone in ovo modifies aggressive behaviors and reproductive performances through alterations of the hypothalamic-pituitary-gonadal axis in the chicken[J]. Animal Reproduction Science,2014,146(3/4):193-201.
[32] 闵红霞,毛思思,黄奕鑫,等. 防御性攻击行为对黑线仓鼠能量收支的影响[J]. 兽类学报,2018,38(2):166-173. Min H X,Mao S S,Huang Y X,et al. Effects of defensive aggression on energy budget of black-line hamsters[J]. Acta Theriologica Sinica,2018,38(2):166-173(in Chinese with English abstract).
[33] Teng W F,Sun W M,Shi L F,et al. Effects of restraint stress on iron,zinc,calcium,and magnesium whole blood levels in mice[J]. Biological Trace Element Research,2008,121(3):243-248.
[34] Li H F,Jiang S X,Yang C,et al. Long-term dexamethasone exposure down-regulates hepatic TFR1 and reduces liver iron concentration in rats[J]. Nutrients,2017,9(6):617.
[35] Gkouvatsos K,Papanikolaou G,Pantopoulos K. Regulation of iron transport and the role of transferrin[J]. Biochimica et Biophysica Acta(BBA):General Subjects,2012,1820(3):188-202.
[36] Dhabhar F S. Effects of stress on immune function:the good,the bad,and the beautiful[J]. Immunologic Research,2014,58(2/3):193-210.
[37] 王免免,周艳婷,张蒙,等. 外源皮质酮对雏鸡法氏囊microRNA表达的影响[J]. 畜牧与兽医,2018,50(5):71-76. Wang M M,Zhou Y T,Zhang M,et al. Effect of exogenous corticosterone on the expression of miRNAs in bursa of fabricius in chicken[J]. Animal Husbandry & Veterinary Medicine,2018,50(5):71-76.
[38] Graham R M,Chua A C G,Herbison C E,et al. Liver iron transport[J]. World Journal of Gastroenterology,2007,13(35):4725-4736.
[39] Anderson E R,Shah Y M. Iron homeostasis in the liver[J]. Comprehensive Physiology,2013,3(1):315-330.
[40] Zhao M,Liu L J,Li X G,et al. Psychological stress leads to hepatic iron accumulation and disturbs iron homeostasis[J]. Journal of Chemical and Pharmaceutical Research,2014,6(5):1128-1134.


 WANG Qing-yun,YANG Wei-ren,YANG Zai-bin,et al.Effects of phytase supplementation on growth performance and nutrients utilization rate in broiler chicks fed diets with different levels of inorganic phosphorus[J].Journal of Nanjing Agricultural University,2009,32(4):124.[doi:10.7685/j.issn.1000-2030.2009.02.025]
 PAN Jia-qiang,LI Jin-chun,TAN Xun,et al.Lipid peroxidatic injury of the cultured pulmonary artery endothelial cells of broiler in vitro[J].Journal of Nanjing Agricultural University,2007,30(4):107.[doi:10.7685/j.issn.1000-2030.2007.02.022]
 YU Zhuo-teng,MAO Sheng-yong,ZHU Wei-yun.Effects of probiotics,synbiotics and antibiotics on VFA production and bacterial community of caecal digesta of broilers[J].Journal of Nanjing Agricultural University,2007,30(4):110.[doi:10.7685/j.issn.1000-2030.2007.03.021]
 LI Jin-chun,TAN Xun,PAN Jia-qiang,et al.Effects of dietary furosemide on incidence of pulmonary hypertension syndrome and pulmonary vascular remodeling in broilers induced by cool environmental temperatures[J].Journal of Nanjing Agricultural University,2006,29(4):97.[doi:10.7685/j.issn.1000-2030.2006.04.020]
 XIANG Rui ping,SUN Wei dong,WANG Jin yong,et al.Effect of cold ambient temperature and T_3 supplementation on free radicals in broilers with pulmonary hypertension syndrome[J].Journal of Nanjing Agricultural University,2003,26(4):79.[doi:10.7685/j.issn.1000-2030.2003.02.019]
 XIANG Rui ping,WANG Xiao long,SUN Wei dong,et al.Acute and chronic pulmonary hypertension syndrome induced by excess sodium from drinking water in broilers[J].Journal of Nanjing Agricultural University,2002,25(4):80.[doi:10.7685/j.issn.1000-2030.2002.03.019]
 Sun Weidong,Wang Xiaolong,Wang Jinyong,et al.Dynamic changes of pulmonary arterial pressure and electrocardiogram after intravenous infusion with L NAME,SNP in broiler chickens[J].Journal of Nanjing Agricultural University,2001,24(4):73.[doi:10.7685/j.issn.1000-2030.2001.03.018]
 Xiang Ruiping,Wang Xiaolong,Wang Jinyong,et al.Muscularization of pulmonary arterioles in field cases of broilers with pulmonary hypertension syndrome[J].Journal of Nanjing Agricultural University,2001,24(4):85.[doi:10.7685/j.issn.1000-2030.2001.01.021]
 YU Lei,RAO Kaiqing,DONG Chuanhao,et al.Comparison analysis of corticosterone deposition in fertilized eggs and embryonic expression of corticosterone metabolic enzymes between Tibetan chickens and broiler chickens[J].Journal of Nanjing Agricultural University,2015,38(4):339.[doi:10.7685/j.issn.1000-2030.2015.02.024]


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