[1]宋昕昊,杨丹,季春雷,等.马度米星铵对克氏原鳌虾肝胰腺药物代谢酶活性及其基因表达的影响[J].南京农业大学学报,2020,43(4):720-727.[doi:10.7685/jnau.201909009]
 SONG Xinhao,YANG Dan,JI Chunlei,et al.Effects of maduramicin on the activity and gene expression level of hepatopancreas drug-metabolizing enzymes of Procambarus clarkia[J].Journal of Nanjing Agricultural University,2020,43(4):720-727.[doi:10.7685/jnau.201909009]
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马度米星铵对克氏原鳌虾肝胰腺药物代谢酶活性及其基因表达的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
43卷
期数:
2020年4期
页码:
720-727
栏目:
动物科学
出版日期:
2020-07-13

文章信息/Info

Title:
Effects of maduramicin on the activity and gene expression level of hepatopancreas drug-metabolizing enzymes of Procambarus clarkia
作者:
宋昕昊 杨丹 季春雷 王佩蓉 张静静 彭麟 高修歌 季辉 江善祥
南京农业大学动物医学院, 江苏 南京 210095
Author(s):
SONG Xinhao YANG Dan JI Chunlei WANG Peirong ZHANG Jingjing PENG Lin GAO Xiuge JI Hui JIANG Shanxiang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
关键词:
马度米星铵克氏原鳌虾药物代谢酶肝胰腺
Keywords:
maduramicinProcambarus clarkiadrug-metabolizing enzymehepatopancreas
分类号:
S859.7
DOI:
10.7685/jnau.201909009
摘要:
[目的] 本文旨在探究马度米星铵对克氏原鳌虾肝胰腺药物代谢酶的影响。[方法] 将192尾成年雄性克氏原鳌虾(体质量21 g左右)随机分为4组(每组48尾)。除对照组外,各处理组的克氏原螯虾分别采用低剂量(0.7 mg·L-1)、中剂量(3.5 mg·L-1)和高剂量(7.0 mg·L-1)的马度米星铵连续药浴给药28 d。分别在给药后7、14、21和28 d每个处理处死12尾克氏原鳌虾,取其肝胰腺,制备微粒体后,测定细胞色素P450(CYP450)、细胞色素b5(CYP b5)含量,Ⅰ相药物代谢酶红霉素-N-脱甲基酶(ERND)活性、氨基比林-N-脱甲基酶(APND)活性和苯胺-4-羟化酶(AH)活性以及Ⅱ相药物代谢酶谷胱甘肽S-转移酶(GST)、尿苷二磷酸葡萄糖醛酸转移酶(UGT)、N-乙酰基转移酶(NAT)的活性,并采用实时荧光定量PCR方法测定CYP450gstCYP b5基因的mRNA表达水平。[结果] 在28 d内,7.0 mg·L-1处理组各时间段CYP450含量与对照组相比显著增加(P<0.05),CYP b5无显著变化(P>0.05);与对照组相比,7.0 mg·L-1处理组的Ⅰ相药物代谢酶ERND、APND、AH活性和Ⅱ相药物代谢酶GST、UGT和NAT活性均显著升高(P<0.05),3.5 mg·L-1处理组的ERND、UGT和NAT活性显著升高(P<0.05),0.7 mg·L-1处理组ERND活性显著升高(P<0.05);马度米星铵对肝胰腺药物代谢酶相关的gstCYP450基因表达均显著上调(P<0.05),CYP b5基因除7 d显著上调外(P<0.05),其余时间无显著变化(P>0.05)。[结论] 马度米星铵可以诱导克氏原鳌虾肝胰腺药物代谢酶,其中ERND和GST对马度米星铵较敏感,可以作为早期马度米星铵污染水体的生物标记物。
Abstract:
[Objectives] This study aimed to investigate the effects of maduramicin on the hepatopancreatic drug-metabolizing enzymes of Procambarus clarkia.[Methods] 192 adult male P. clarkia(body mass about 21 g) were randomly divided into four groups with 48 tails per group. The P. clarkia in other three treated groups were dipped bath for successive 28 days at low dose group(0.7 mg·L-1),middle dose group(3.5 mg·L-1) and high dose group(7.0 mg·L-1),respectively. Hepatopancreas of P. clarkia were collected at 7,14,21 and 28 d to determine the contents of cytochrome P450(CYP450) and cytochrome b5(CYP b5),the activities of the Ⅰ phase drug metabolic enzymes including erythromycin-N-demethylase(ERND),aminopyrine-N-demethylase(APND),aniline-4-hydroxylase(AH),and the activities of the Ⅱ phase drug metabolic enzymes including glutathione S-transferase(GST),uridine diphospho-glucuronyl transferase(UGT) and N-acetyltransferase(NAT). The mRNA expression levels of CYP450,gst and CYP b5 were detected by real time qPCR.[Results] During whole exposure duration,the content of CYP450 of P. clarkia treated by maduramicin at 7 mg·L-1 was significantly higher than that in the control group(P<0.05),and CYP b5 had no significant change(P>0.05). Compared with the control group,the activities of ERND,APND,AH,GST,UGT and NAT in the 7.0 mg·L-1 treatment group significantly increased(P<0.05). The activities of ERND,UGT and NAT in the 3.5 mg·L-1 treatment group significantly increased(P<0.05),and the activity of ERND in the 0.7 mg·L-1 treatment group significantly increased(P<0.05). Both gst and CYP450 genes related to liver drug enzyme were significantly up-regulated by maduramicin(P<0.05),and CYP b5 gene was significantly up-regulated except at 7 days(P<0.05),but there was no significant change at the rest of the time(P>0.05).[Conclusions] Maduramicin can induce the hepatopancreas drug-metabolizing enzymes of P. clarkia hepatopancreas. ERND and GST are more sensitive to maduramicin and can be used as biomarkers for early maduramicin contamination.

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 YANG Dan,SONG Xinhao,JI Chunlei,et al.Effects of maduramicin on hepatic cytochrome P450 enzymes of Carassius auratus[J].Journal of Nanjing Agricultural University,2020,43(4):927.[doi:10.7685/jnau.201911012]

备注/Memo

备注/Memo:
收稿日期:2019-09-04。
基金项目:国家自然科学基金项目(31672612)
作者简介:宋昕昊,硕士研究生。
通信作者:江善祥,教授,博导,主要从事新兽药创制及药理毒理学研究,E-mail:nauvy@sina.com。
更新日期/Last Update: 1900-01-01