[1]王玉龙,张存政,刘凤权.重金属汞的抗体制备及免疫分析研究进展[J].南京农业大学学报,2020,43(5):800-807.[doi:10.7685/jnau.202006006]
 WANG Yulong,ZHANG Cunzheng,LIU Fengquan.Research progress in antibody developments and immunoassays for heavy metal mercury[J].Journal of Nanjing Agricultural University,2020,43(5):800-807.[doi:10.7685/jnau.202006006]
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重金属汞的抗体制备及免疫分析研究进展()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
43卷
期数:
2020年5期
页码:
800-807
栏目:
综述
出版日期:
2020-09-15

文章信息/Info

Title:
Research progress in antibody developments and immunoassays for heavy metal mercury
作者:
王玉龙1 张存政1 刘凤权2
1. 江苏省农业科学院农产品质量安全与营养研究所, 江苏 南京 210014;
2. 江苏省农业科学院植物保护研究所, 江苏 南京 210014
Author(s):
WANG Yulong1 ZHANG Cunzheng1 LIU Fengquan2
1. Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;(;
2. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
关键词:
免疫分析半抗原
Keywords:
mercuryimmunoassayhaptens
分类号:
X830.2
DOI:
10.7685/jnau.202006006
摘要:
重金属汞免疫检测方法具有操作简单、敏感性高等优点,可作为传统仪器检测的辅助工具,用于样品中汞浓度的高通量筛查。汞的半抗原、抗体和反应信号检测体系是汞免疫分析的关键。研究人员先后基于汞-螯合剂和汞-谷胱甘肽等半抗原,制备了不同性能的汞抗体,并构建了多种汞的快速免疫检测方法。本文从抗体制备角度,重点对汞半抗原结构设计以及抗体结合特性研究进行综述,同时对基于不同信号转换的免疫分析方法研究进展进行介绍,并对其未来发展趋势进行展望。
Abstract:
Immunoassay for heavy metal mercury has the advantages of simple operation and high sensitivity,which can be used to screen the concentration of Hg in samples with high throughput and finally to be used as assistive tools for traditional instrument detection. Haptens,antibodies and responding signal detection systems of Hg are the key to Hg immunodetection. Based on the haptens of Hg-chelating agents and Hg-glutathione,the researchers have prepared mercury antibodies with different properties and constructed a variety of rapid immunoassays for mercury. In this paper,the structure design and antibody binding properties of mercury hapten were reviewed from the perspective of antibody preparation. Meanwhile,the research progress of immunoassay based on different signal transformation was introduced,and the future development trend was prospected as well.

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备注/Memo

备注/Memo:
收稿日期:2020-06-05。
基金项目:国家重点研发计划项目(2019YFC1605902,2019YFC1605901);国家自然科学基金项目(31801766)
作者简介:王玉龙,助理研究员,研究方向为小分子快速免疫检测,E-mail:yllzzn@sina.com。
通信作者:刘凤权,研究员,研究方向为植物病理学、农药及重金属免疫检测等,E-mail:fqliu20011@sina.com。
更新日期/Last Update: 1900-01-01