[1]王晓莉,李哲,叶文武,等.江苏省13个地区水稻种子携带4种不同恶苗病菌的LAMP检测[J].南京农业大学学报,2020,43(5):846-852.[doi:10.7685/jnau.201912029]
 WANG Xiaoli,LI Zhe,YE Wenwu,et al.Detection of 4 different rice bakanae pathogens on rice seeds collected from 13 regions of Jiangsu Province using LAMP assays[J].Journal of Nanjing Agricultural University,2020,43(5):846-852.[doi:10.7685/jnau.201912029]
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江苏省13个地区水稻种子携带4种不同恶苗病菌的LAMP检测()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
43卷
期数:
2020年5期
页码:
846-852
栏目:
植物科学
出版日期:
2020-09-15

文章信息/Info

Title:
Detection of 4 different rice bakanae pathogens on rice seeds collected from 13 regions of Jiangsu Province using LAMP assays
作者:
王晓莉1 李哲1 叶文武1 杨红福2 庄义庆2 郑小波1
1. 南京农业大学植物保护学院, 江苏 南京 210095;
2. 镇江市农业科学院, 江苏 镇江 212400
Author(s):
WANG Xiaoli1 LI Zhe1 YE Wenwu1 YANG Hongfu2 ZHUANG Yiqing2 ZHENG Xiaobo1
1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;
2. Zhenjiang Agric-academy of Sciences, Zhenjiang 212400, China
关键词:
水稻恶苗病种子带菌检测环介导等温扩增技术(LAMP)
Keywords:
rice bakanaedetection of seed-borne pathogensloop-mediated isothermal amplification(LAMP)
分类号:
S432.4+4
DOI:
10.7685/jnau.201912029
摘要:
[目的] 水稻种子携带病原菌是水稻恶苗病的重要传播途径。本研究旨在了解当前江苏省栽培水稻品种种子携带恶苗病菌的状况。[方法] 采用可分别特异性识别引起水稻恶苗病的藤仓镰孢(Fusarium fujikuroi)、层出镰孢(F.proliferatum)、拟轮枝镰孢(F.verticilliodes)和F.andiyazi的LAMP检测技术,对江苏省2017年收获的103份水稻种子携带上述4种病原菌的情况进行检测。[结果] 在103份稻种样本中,有89份检测出携带水稻恶苗病菌。其中,检出率最高的是藤仓镰孢,为83.5%,层出镰孢、拟轮枝镰孢和F.andiyazi也被检测到,但检出率较低,依次为15.5%、1.9%和1.9%。[结论] 江苏省水稻种子携带恶苗病菌是一种普遍现象,藤仓镰孢是江苏水稻恶苗病的优势致病菌种,其次是层出镰孢。种子带菌是上述2种病原菌所致恶苗病的重要初侵染源。
Abstract:
[Objectives] Rice seeds carrying pathogens is an important way of spreading rice bakanae pathogens. The research aimed to reveal the situation of seed-borne rice bakanae pathogens in Jiangsu Province,China.[Methods] The seeds of 103 rice cultivars harvested from Jiangsu Province in 2017 were analyzed by loop-mediated isothermal amplification (LAMP) assays for specific detection of four known rice bakanae pathogen species,including Fusarium fujikuroi,F.proliferatum,F.verticilliodes and F.andiyazi.[Results] Among the 103 seed samples,89 seed samples were detected with rice bakanae pathogens. The most frequent species was F.fujikuroi,representing 83.5% of the total samples tested. The detection rates of F.proliferatum,F.verticillides,and F.andiyazi were low,i.e.,15.5%,1.9% and 1.9%,respectively.[Conclusions] Rice seeds carrying rice bakanae pathogens is common in Jiangsu Province,and F.fujikuroi is the most dominant pathogen,followed by F.proliferatum. Seed-borne of the two pathogens are an important primary infection source of rice bakanae. The results of this study provide important reference value for prevention and treatment of the rice bakanae in Jiangsu Province.

参考文献/References:

[1] Singh R,Sunder S. Foot rot and bakanae of rice:an overview[J]. Review of Plant Pathology,2012,5:566-604.
[2] 郑红明. 2016年我国水稻生产发展报告[J]. 中国粮食经济,2016(12):37-40. Zheng H M. Report on the development of rice production in China in 2016[J]. China Grain Economy,2016(12):37-40(in Chinese).
[3] Picco A M,Lorenzi E,Rodolfi M,et al. A multi-location mycological survey on some Italian rice varieties during the crop season[C]//Atti,Giornate Fitopatologiche,Baselga Di Piné. Trento,Italy,2002:7-11.
[4] 马晓伟. 藤仓镰孢菌对多菌灵抗药性的分子机制研究[D]. 南京:南京农业大学,2012. Ma X W. Molecular resistant mechanism of Fusarium fujikuroi against carbendazim[D]. Nanjing:Nanjing Agricultural University,2012(in Chinese with English abstract).
[5] 季芝娟,马良勇,李西明,等. 水稻品种恶苗病抗性快速鉴定方法研究[J]. 浙江农业科学,2008(1):89-91. Ji Z J,Ma L Y,Li X M,et al. Rapid identification of resistance of rice varieties to rice bakanae disease[J]. Journal of Zhejiang Agricultural Sciences,2008(1):89-91(in Chinese).
[6] Sürek H,Gümüstekin H. Research activities on controlling rice bakanae and foot rot disease(Fusarium moniliforme)in Turkey[J]. Cahiers Options Méditerranéennes,1994,8(2):27-30.
[7] Wulff E G,S?rensen J L,Lübeck M,et al. Fusarium spp. associated with rice Bakanae:ecology,genetic diversity,pathogenicity and toxigenicity[J]. Environmental Microbiology,2010,12(3):649-657.
[8] 陈利锋,徐敬友. 农业植物病理学:南方本[M]. 北京:中国农业出版社,2001. Chen L F,Xu J Y. Agricultural Phytopathology:The Southern[M]. Beijing:China Agriculture Press,2001(in Chinese).
[9] 方兴洲,陈莉,产祝龙,等. 水稻恶苗病与浸种、催芽和播种等因子的关系研究[J]. 热带作物学报,2012,33(6):1107-1110. Fang X Z,Chen L,Chan Z L,et al. The relationship of seed soaking,germination and sowing to rise bakanae disease[J]. Chinese Journal of Tropical Crops,2012,33(6):1107-1110(in Chinese with English abstract).
[10] Leslie J F,Zeller K A. Heterokaryon incompatibility in fungi:more than just another way to die[J]. Journal of Genetics,1996,75:415-424.
[11] 李小林,谷安宇,张馨宇,等. 水稻种子寄藏真菌的3种检测方法对比研究[J]. 中国农业大学学报,2011,16(6):43-51. Li X L,Gu A Y,Zhang X Y,et al. Comparative analysis of three kinds of testing methods for rice seed borne fungi[J]. Journal of China Agricultural University,2011,16(6):43-51(in Chinese with English abstract).
[12] 胡兰,姜钰,徐秀德. 基于形态学和EF-1α序列特征的我国高粱子粒寄藏镰孢菌种群鉴定[J]. 作物杂志,2013(4):129-132. Hu L,Jiang Y,Xu X D. Identification of sorghum seed-borne Fusarium population based on fungi morphology and EF-1α sequence[J]. Crops,2013(4):129-132(in Chinese with English abstract).
[13] Valasek M A,Repa J J. The power of real-time PCR[J]. Advances in Physiology Education,2005,29(3):151-159.
[14] Notomi T,Okayama H,Masubuchi H,et al. Loop-mediated isothermal amplification of DNA[J]. Nucleic Acids Research,2000,28(12):63.
[15] 许苗,冯慧,王淑琛,等. 基于环介导等温扩增技术检测江苏省中部地区小麦赤霉病病菌的种群组成[J]. 麦类作物学报,2018,38(4):498-504. Xu M,Feng H,Wang S C,et al. Detection of Fusarium spp. causing wheat scab in the middle area of Jiangsu using LAMP assay[J]. Journal of Triticeae Crops,2018,38(4):498-504(in Chinese with English abstract).
[16] 袁咏天,戎振洋,叶文武,等. 应用环介导等温扩增技术检测江苏水稻种子携带的水稻恶苗病菌[J]. 中国水稻科学,2018,32(5):493-500. Yuan Y T,Rong Z Y,Ye W W,et al. Detection of seed-borne rice bakanae pathogens in Jiangsu Province,China using loop-mediated isothermal amplification assays[J]. Chinese Journal of Rice Science,2018,32(5):493-500(in Chinese with English abstract).
[17] Rong Z Y,Yuan Y T,Ye W W,et al. Rapid diagnosis of rice bakanae caused by Fusarium fujikuroi and F.proliferatum using loop-mediated isothermal amplification assays[J]. Journal of Phytopathology,2018,166(4):283-290.
[18] 曾丹丹,戎振洋,袁咏天,等. LAMP快速诊断由拟轮枝镰孢引起的水稻恶苗病[J]. 南京农业大学学报,2018,41(2):286-292. DOI:10.7685/jnau. 201705023. Zeng D D,Rong Z Y,Yuan Y T,et al. Rapid diagnosis of rice bakanae disease caused by Fusarium verticillioides using a loop-mediated isothermal amplification assay[J]. Journal of Nanjing Agricultural University,2018,41(2):286-292(in Chinese with English abstract).
[19] 戎振洋,袁咏天,曾丹丹,等. 基于环介导等温扩增技术快速诊断由Fusarium andiyazi引起的水稻恶苗病[J]. 植物病理学报,2018,48(2):256-262. Rong Z Y,Yuan Y T,Zeng D D,et al. Rapid diagnosis of rice bakanae caused by Fusarium andiyazi using a loop-mediated isothermal amplification assay[J]. Acta Phytopathologica Sinica,2018,48(2):256-262(in Chinese with English abstract).
[20] Kim M H,Hur Y J,Lee S B,et al. Large-scale screening of rice accessions to evaluate resistance to bakanae disease[J]. Journal of General Plant Pathology,2014,80:408-414.
[21] 王建林,姜海平,管信山,等. 如东县水稻恶苗病重发原因及综合防治对策[J]. 上海农业科技,2014(1):114-115. Wang J L,Jiang H P,Guan X S,et al. The causes and comprehensive control measures of severe rice bakanae disease in Rudong County[J]. Shanghai Agricultural Science and Technology,2014(1):114-115(in Chinese).
[22] 刘永忠,李华. 靖江市水稻恶苗病发生原因分析与防治对策探讨[J]. 上海农业科技,2014(1):117-118. Liu Y Z,Li H. Analysis on the causes of rice bakanae and its control measures in Jingjiang City[J]. Shanghai Agricultural Science and Technology,2014(1):117-118(in Chinese).

相似文献/References:

[1]陈长军,周明国,叶钟音,等.肥床旱育水稻恶苗病的发生原因初探[J].南京农业大学学报,1997,20(2):42.[doi:10.7685/j.issn.1000-2030.1997.02.009]
 Chen Changjun,Zhou Mingguo,Ye Zhongyin,et al.CAUSES OF RICE BAKANAE ( FUSARIUM MONILIFORME) OCCURRANCE IN FERTILE AND DRY SEEDBED[J].Journal of Nanjing Agricultural University,1997,20(5):42.[doi:10.7685/j.issn.1000-2030.1997.02.009]
[2]曾丹丹,戎振洋,袁咏天,等.LAMP快速诊断由拟轮枝镰孢引起的水稻恶苗病[J].南京农业大学学报,2018,41(2):286.[doi:10.7685/jnau.201705023]
 ZENG Dandan,RONG Zhenyang,YUAN Yongtian,et al.Rapid diagnosis of rice bakanae disease caused by Fusarium verticillioides using a loop-mediated isothermal amplification assay[J].Journal of Nanjing Agricultural University,2018,41(5):286.[doi:10.7685/jnau.201705023]

备注/Memo

备注/Memo:
收稿日期:2019-12-19。
基金项目:国家863计划项目(2012AA101501);江苏省农业科技自主创新资金项目[cx(17)3023]
作者简介:王晓莉,实验师,主要从事卵菌与真菌分子生物学研究。
通信作者:郑小波,教授,主要从事卵菌与真菌分子遗传研究,E-mail:xbzheng@njau.edu.cn。
更新日期/Last Update: 1900-01-01