[1]殷涂童,周倩倩,张静,等.Bacillus halotolerans G20联合生物炭及钾长石对生菜吸收镉和改良土壤的影响[J].南京农业大学学报,2020,43(5):869-876.[doi:10.7685/jnau.202001034]
 YIN Tutong,ZHOU Qianqian,ZHANG Jing,et al.Synergistic effect of Bacillus halotolerans G20 and biochar and K-feldspar on Cd uptake of lettuce and remediation in Cd-contaminated soils[J].Journal of Nanjing Agricultural University,2020,43(5):869-876.[doi:10.7685/jnau.202001034]
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Bacillus halotolerans G20联合生物炭及钾长石对生菜吸收镉和改良土壤的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
43卷
期数:
2020年5期
页码:
869-876
栏目:
生物与环境
出版日期:
2020-09-15

文章信息/Info

Title:
Synergistic effect of Bacillus halotolerans G20 and biochar and K-feldspar on Cd uptake of lettuce and remediation in Cd-contaminated soils
作者:
殷涂童 周倩倩 张静 张亚见 盛下放 何琳燕
南京农业大学生命科学学院/农业农村部农业环境微生物重点实验室, 江苏 南京 210095
Author(s):
YIN Tutong ZHOU Qianqian ZHANG Jing ZHANG Yajian SHENG Xiafang HE Linyan
College of Life Sciences/Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing 210095, China
关键词:
固体菌剂生菜品质Cd含量Cd污染土壤
Keywords:
combined bacteria agentlettuce qualityCd contentCd pollution soil
分类号:
Q939.96
DOI:
10.7685/jnau.202001034
摘要:
[目的] 本文旨在探究细菌与不同钝化剂联合阻控生菜吸收重金属、修复重金属污染土壤的作用。[方法] 采集南京市某矿区镉污染土壤为盆栽试验土壤,选用生物炭(B)和钾长石(K)作为钝化剂与镉抗性细菌Bacillus halotolerans G20菌株联合,探究不同处理对生菜(Lactuca sativa L.var.ramosa Hort.)地上部干质量、Cd含量、营养品质和根际土壤pH值、有机质含量、Cd形态变化的影响。[结果] 与对照相比,菌株G20、生物炭、钾长石及其固体菌剂均能增加生菜地上部干质量,降低生菜Cd含量。其中,菌株G20与生物炭制备的固体菌剂(G20L+B)使生菜地上部Cd含量显著降低53.1%,降至0.15 mg·kg-1,低于国家食品安全生产限量标准。该固体菌剂处理还能显著提高生菜根际土壤细菌数量(16.5%)、pH值(0.3)和有机质含量(44.6%),降低根际土壤有效态Cd含量(45.4%),显著增加生菜中维生素C含量(32.9%),降低亚硝酸盐含量(38.8%),减轻Cd对生菜的毒害,提高生菜品质。[结论] B.halotolerans G20与生物炭制备的固体菌剂具有修复中度Cd污染土壤,保障蔬菜安全生产的潜力。
Abstract:
[Objectives] The objective of this study was to explore the role of bacteria and different passivation agents in reducing the heavy metals uptake of vegetables and remediating heavy metal contaminated soil.[Methods] The cadmium-contaminated soil in a mining area in Nanjing was collected as potting test soil. In this pot experiment,biochar (B) and K-feldspar (K) were used as passivators in combination with the cadmium-resistant bacteria Bacillus halotolerans G20 strain to explore the effects of different treatments on the growth,Cd content and quality of lettuce (Lactuca sativa L. var. ramosa Hort.) in Cd-contaminated soil,as well as pH,organic matter content and Cd morphological changes of lettuce rhizosphere soils.[Results] Compared with the control,strain G20,biochar,K-feldspar and their combined bacteria agents could increase the above-ground dry weight and reduce the Cd content of lettuce. Among them,the Cd content in the above-ground tissue of lettuce treated with biochar combined bacteria agent (G20L+B) was significantly reduced by 53.1% to 0.15 mg·kg-1,which was lower than the national food safety limit. G20+B agent could increase the bacterial count (16.5%),pH value (0.3) and organic matter content of the lettuce rhizosphere soil (44.6%),reduce the effective Cd content (45.4%),reduce the toxicity of Cd to the lettuce,significantly increase the vitamin C content in the lettuce (32.9%),and reduce nitrite content (38.8%).[Conclusions] The biochar combined B.halotolerans G20 bacteria agent had the potential to repair moderately Cd-contaminated soil and to ensure the safe production of vegetables.

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备注/Memo

备注/Memo:
收稿日期:2020-01-15。
基金项目:国家自然科学基金项目(41977199,41471273);农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室开放基金项目(16nybcdhj-8);江苏省社会发展项目(BE2016744)
作者简介:殷涂童,硕士研究生。
通信作者:何琳燕,教授,主要从事重金属污染土壤的生物修复研究,E-mail:helyan0794@njau.edu.cn。
更新日期/Last Update: 1900-01-01