[1]薛佳婧,杨洁,宋瑶,等.不同污染强度下PAHs降解基因在土壤-根表-植物系统中的分布[J].南京农业大学学报,2020,43(5):877-886.[doi:10.7685/jnau.201912016]
 XUE Jiajing,YANG Jie,SONG Yao,et al.Distribution of key genes responsible for PAHs degradation in soil-root- surface-plant systems under different levels of PAHs pollution[J].Journal of Nanjing Agricultural University,2020,43(5):877-886.[doi:10.7685/jnau.201912016]
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不同污染强度下PAHs降解基因在土壤-根表-植物系统中的分布()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
43卷
期数:
2020年5期
页码:
877-886
栏目:
生物与环境
出版日期:
2020-09-15

文章信息/Info

Title:
Distribution of key genes responsible for PAHs degradation in soil-root- surface-plant systems under different levels of PAHs pollution
作者:
薛佳婧 杨洁 宋瑶 孙凤飞 刘娟 高彦征
南京农业大学资源与环境科学学院, 江苏 南京 210095
Author(s):
XUE Jiajing YANG Jie SONG Yao SUN Fengfei LIU Juan GAO Yanzheng
College of Resource and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
关键词:
多环芳烃根箱紫花苜蓿根际土壤PAHs降解基因
Keywords:
polycyclic aromatic hydrocarbon(PAHs)root boxalfalfarhizosphere soilgenes responsible for PAHs degradation
分类号:
X172
DOI:
10.7685/jnau.201912016
摘要:
[目的] 本文旨在解析植物-微生物联合修复多环芳烃(PAHs)污染土壤的分子机制,并为在PAHs污染区生产安全的农产品提供技术支持和理论依据。[方法] 以紫花苜蓿为供试植物,以菲和芘作为供试PAHs,以PAHs降解菌株Sphingobium sp.RS2为供试菌株,在根箱装置中进行温室盆栽试验,通过高效液相色谱技术和实时荧光定量PCR技术,研究不同PAHs污染浓度对种植紫花苜蓿的土壤-根表-植物系统中PAHs降解基因丰度和分布的影响。[结果] 高浓度PAHs污染对紫花苜蓿的生长有抑制作用,而接种功能菌株RS2可以促进紫花苜蓿的生长。与未接种RS2的对照组相比,接种RS2的处理组紫花苜蓿生物量平均增加约22.4%。根际土壤中PAHs残留量显著低于非根际土壤。随PAHs污染浓度增加,降解基因丰度也增加。根表部位降解基因丰度最高,各层室土壤次之,植物根部和茎叶部丰度最低;在同一采样区域中,phe基因丰度显著高于nahAcnidA基因。菲、芘残留量与nahAc基因丰度呈显著正相关。[结论] 在土壤-根表-植物系统中,PAHs降解基因的丰度受PAHs浓度的影响,且根表环境中PAHs降解基因丰度最高,是PAHs代谢最为旺盛的区域。根表的PAHs代谢在去除土壤PAHs污染以及减低植物PAHs污染风险中起着不可替代的作用。
Abstract:
[Objectives] This study was performed to further analyze the molecular mechanisms involved in soil remediation contaminated by polycyclic aromatic hydrocarbon (PAHs) with the plant-microorganism combined technology,and to provide the technical support and theoretical basis for the production of safe agricultural products in PAH-contaminated areas.[Methods] The distribution of key genes responsible for PAHs degradation in soil-root-surface-plant systems under different levels of PAHs pollution were studied by HPLC and real-time quantitative PCR. Alfalfa was used as the target plant to conduct greenhouse potted experiments in the root box device. Meanwhile,phenanthrene and pyrene were used as the representatives of PAHs and an efficient PAH-degrading strain,RS2,was used for inoculation with alfalfa.[Results] High levels of PAHs contamination could inhibit the growth of alfalfa,while the inoculation of RS2 with alfalfa could promote the plant growth. The biomass of alfalfa inoculated with RS2 increased by 22.4% in average compared with the RS2-free treatments. The total PAHs contents in rhizosphere soils were significantly lower than those in non-rhizosphere soils. The copy numbers of genes responsible for PAHs degradation increased with the increase of soil PAHs concentration. Furthermore,the abundances of these genes on root surfaces were the highest,followed by those in soils,and the abundances of these genes in stems and roots were the least. In the same sampling area,the copy number of phe gene was significantly higher than those of nahAc or nidA. Under different levels of contamination,the residue concentration of phenanthrene or pyrene were positively correlated with the copy number of nahAc gene.[Conclusions] The abundances of genes responsible for PAHs degradation were affected by the PAHs contents in the soil-root-surface-plant systems,and the highest gene abundance was found in the root surface environment,which was the most active region for PAHs metabolism. PAHs metabolism on root surfaces played an irreplaceable role in removing PAHs contaminants from soils and reducing the risk of PAHs contamination in plants.

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备注/Memo

备注/Memo:
收稿日期:2019-12-10。
基金项目:国家自然科学基金项目(31770549,41771523,41877125)
作者简介:薛佳婧,硕士研究生。
通信作者:刘娟,副教授,主要研究方向为土壤有机污染控制与修复,E-mail:liujuan@njau.edu.cn。
更新日期/Last Update: 1900-01-01