[1]马宁,刘艳霞,李想,等.基于熵权法综合评价植物根际促生菌对烟草的促生作用[J].南京农业大学学报,2020,43(5):887-895.[doi:10.7685/jnau.201910046]
 MA Ning,LIU Yanxia,LI Xiang,et al.Comprehensive evaluation of growth promotion effect of PGPR strains on tobacco based on entropy weight method[J].Journal of Nanjing Agricultural University,2020,43(5):887-895.[doi:10.7685/jnau.201910046]
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基于熵权法综合评价植物根际促生菌对烟草的促生作用()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
43卷
期数:
2020年5期
页码:
887-895
栏目:
生物与环境
出版日期:
2020-09-15

文章信息/Info

Title:
Comprehensive evaluation of growth promotion effect of PGPR strains on tobacco based on entropy weight method
作者:
马宁1 刘艳霞2 李想2 陈雪3 杨兴明1
1. 南京农业大学资源与环境科学学院, 江苏 南京 210095;
2. 贵州省烟草科学研究院, 贵州 贵阳 550000;
3. 贵州省烟草公司毕节分公司, 贵州 毕节 551700
Author(s):
MA Ning1 LIU Yanxia2 LI Xiang2 CHEN Xue3 YANG Xingming1
1. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;
2. Guizhou Tobacco Research Institute, Guiyang 550000, China;
3. Bijie Company of Guizhou Tobacco Corporation, Bijie 551700, China
关键词:
烟草促生PGPR熵权法评价指标
Keywords:
tobaccopromotingPGPRentropy weight methodevaluation index
分类号:
S144.2
DOI:
10.7685/jnau.201910046
摘要:
[目的] 本文旨在筛选高效植物根际促生菌(PGPR),考察PGPR对烟草幼苗、成苗的促生作用,为研发烟草微生物肥料提供可靠材料。[方法] 以从烟草根际分离的25株功能菌为供试菌株,通过测定菌株产氰化氢(HCN)、生物固氮、溶磷、解钾、分泌吲哚乙酸(IAA)等能力,得到不同菌株的功能特性;通过烟草的各项生长指标,探究PGPR菌株对烟草的促生作用;采用熵权法对烟草生长状态的多性状进行客观评价。[结果] 25株PGPR菌株产HCN、生物固氮、溶磷、解钾、产IAA能力各有不同。除菌株L2外,供试PGPR均能促进烟草成苗根系和地上部生长,其中菌株PS-S和F2促生效果最好,菌株L25和VC48次之,随后是菌株W1。采用熵权法得到根系活力的权重为0.034,较其他性状大,对评价结果有更大的影响。[结论] 经综合评价,菌株W1、F2、VC48和LX5共4株高效PGPR菌株兼具多种功能特性,同时可以显著促进烟草生长,有望进一步研究开发成为微生物肥料生产菌种。
Abstract:
[Objectives] The effects of plant growth-promoting rhizobacteria (PGPR) on tobacco seedling and seedling growth were investigated by screening high efficiency and multifunctional PGPR,which provided reliable materials for the development of tobacco microbial fertilizer.[Methods] 25 strains bacteria from tobacco rhizosphere in the early stage were used as tested strains bacteria,and the abilities of producing hydrogen cyanide (HCN),biological nitrogen fixation,phosphate solubilization,potassium solution and to secrete indole-3-aceticacid were determined. The different functional characteristics of strains were obtained,and the promoting effects of the strains on tobacco were investigated by measuring the data of various indexes of tobacco. In order to solve the problems of strong subjectivity,single evaluation standard and non-intuitive results in the evaluation method of tobacco growth state,entropy weight method was used to objectively evaluate the multiple traits of tobacco growth state.[Results] The biological nitrogen fixation,phosphorus solubilization,potassium solution and IAA production ability of 25 PGPR strains were different. Except strain L2,the tested PGPR could promote the growth of tobacco seedling roots and shoots. The effects of strain PS-S and F2 were the best,strain L25 and VC48 were the second,followed by strain W1. They were obtained by entropy weight method,and the weight of root activity of 0.034 was larger than those of other traits,which had a greater influence on the evaluation results.[Conclusions] After comprehensive evaluation,four high-efficiency PGPR strains W1,F2,VC48 and LX5,had multiple functional characteristics and could significantly promote tobacco growth. They were expected to be further developed into microbial fertilizer production strains.

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备注/Memo

备注/Memo:
收稿日期:2019-10-28。
基金项目:贵州省烟草公司科技项目(201703);贵州省烟草公司毕节市公司科技项目(201710)
作者简介:马宁,硕士研究生。
通信作者:杨兴明,教授,研究方向为土壤微生物与植物营养,E-mail:xingming@njau.edu.cn;陈雪,高级农艺师,主要从事烤烟生产技术,E-mail:248366944@qq.com。
更新日期/Last Update: 1900-01-01