[1]毛志强,焦姣,魏婷婷,等.一种新型生物延缓剂对小麦穗发芽及其籽粒品质的影响[J].南京农业大学学报,2020,43(6):998-1005.[doi:10.7685/jnau.202004011]
 MAO Zhiqiang,JIAO Jiao,WEI Tingting,et al.Effect of biological retardant on the pre-harvest sprouting and quality of wheat grains[J].Journal of Nanjing Agricultural University,2020,43(6):998-1005.[doi:10.7685/jnau.202004011]
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一种新型生物延缓剂对小麦穗发芽及其籽粒品质的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
43卷
期数:
2020年6期
页码:
998-1005
栏目:
植物科学
出版日期:
2020-11-10

文章信息/Info

Title:
Effect of biological retardant on the pre-harvest sprouting and quality of wheat grains
作者:
毛志强 焦姣 魏婷婷 王宇 王悦 赵宪成 周繁
沈阳化工研究院有限公司, 辽宁 沈阳 110021
Author(s):
MAO Zhiqiang JIAO Jiao WEI Tingting WANG Yu WANG Yue ZHAO Xiancheng ZHOU Fan
Shenyang Research Institute of Chemical Industry Co. Ltd., Shenyang 110021, China
关键词:
小麦生物延缓剂穗发芽α-淀粉酶淀粉蛋白质
Keywords:
wheatbiological retardantpre-harvest sproutingα-amylase activitystarchprotein
分类号:
S512.1
DOI:
10.7685/jnau.202004011
摘要:
[目的] 针对小麦穗发芽导致减产降质的农业气候问题,探究生物延缓剂延缓小麦穗发芽及其对小麦籽粒品质的影响,旨在为该生物延缓剂延缓小麦穗发芽提供理论依据,并为下一步研究和推广奠定基础。[方法] 选用河南开封主栽小麦品种‘泛麦8号’为试验材料,在小麦蜡熟期取样进行穗发芽诱导,试验设置5个处理和5个取样时间:CK(清水)、P(1%二甲基亚砜)、P+D(1%二甲基亚砜+0.1%渗透剂)、T2(1 500 mg·L-1生物延缓剂+1%二甲基亚砜+0.1%渗透剂)、M4(1 200 mg·L-1茉莉酸甲酯+1%二甲基亚砜+0.1%渗透剂),在浸种4 h后的12、24、36、60、84 h取样,探究不同时间该生物延缓剂对小麦发芽率、千粒质量、α-淀粉酶活性、淀粉及蛋白质含量的影响。[结果] 不同处理的种子发芽率随诱导穗发芽时间延长呈现升高趋势,其中CK、P和P+D处理之间差异不显著;与CK相比,T2和M4的发芽率显著降低,T2的发芽时间延缓了24~32 h,而M4的种子失活,未能发芽。千粒质量随诱导穗发芽时间的延长呈现降低趋势,T2与CK相比缓解了小麦千粒质量降低的趋势。小麦α-淀粉酶活性随诱导穗发芽时间的延长呈现升高趋势,T2的α-淀粉酶活性显著高于M4。降落数值呈现降低趋势,CK、P和P+D处理降低的幅度显著大于T2和M4,且在诱导60 h后达到稳定状态,T2和M4的降落数值在36 h后急剧下降,且T2下降的更为明显。小麦总淀粉、支链淀粉、总蛋白、醇溶蛋白、谷蛋白含量及小麦粉干湿面筋含量及SDS沉降值随小麦穗发芽时间的延长均呈现下降趋势,T2的这些指标在84 h时分别降低16.15%、19.25%、3.17%、5.95%、6.86%、9.58%、5.36%和12.53%,降低的幅度(比率)显著小于CK,而直链淀粉含量未受到显著影响。籽粒清蛋白和球蛋白含量呈现增加趋势,且清蛋白增加幅度大于球蛋白,而不同处理小麦的醇溶蛋白和谷蛋白含量随诱导穗发芽时间的延长呈现下降趋势,诱导84 h时T2和M4与CK相比差异显著,且谷蛋白受发芽的影响更为明显。[结论] 喷施1 500 mg·L-1的生物延缓剂显著延缓小麦的穗发芽时间,保证了小麦种子的活力,且在一定程度上缓解小麦穗发芽导致产量降低和品质下降的负效应。
Abstract:
[Objectives] In order to solve the problem of agricultural climate,which leads to the reduction of yield and quality with the harvest sprouting wheat,a new biological retardant was used to explore the effect of the biological retardant on the pre-harvest sprouting of wheat and the quality of wheat grains,which can provide the theoretical basis for delaying the pre-harvest sprouting of wheat and laying the foundation for the next research and promotion.[Methods] The wheat variety’Fanmai 8’in Kaifeng of Henan Province was selected as the experimental material,including five treatments and five sampling times in this experiment:CK(water),P[1% dimethyl sulfoxide(DMSO)],P+D(1% DMSO+0.1% penetrant),T2(1 500 mg·L-1 biological retardant+1% DMSO+0.1% penetrant),M4(1 200 mg·L-1 methyl jasmonate+1% DMSO+0.1% penetrant) and 12,24,48,72,84 h after seed soaking 4 h. The samples were carried out to explore the effect of biological retardant on delaying the germination rate,1 000-grain weight,α-amylase activity,starch and protein content in wheat grains.[Results] The results indicated that the seed germination rate of wheat showed an increasing trend in different treatments,among which three treatments of CK,P and P+D did not show the significant difference. T2 and M4 significantly reduced the seed germination rate compared with CK,in which the seed germination rate of T2 treatment was delayed about 24-32 h,while seed vitality of wheat was killed by M4 and the grains couldn’t germinate. The 1 000-grain weight decreased significantly with the increase of germination time,in which T2 alleviated the decrease,compared with CK. The α-amylase activity increased with the increase of pre-harvest sprouting time of wheat,and the α-amylase activity of T2 was significantly higher than M4. The falling number value showed a decreasing trend,and the decrease of CK,P,P+D was significantly higher than T2 and M4,and reached a steady state after the induction of 60 hours. T2 and M4 showed a sharp decline after 36 hours,in which the T2 decreased more significantly. The content of total starch,amylopectin,total protein,gliadin,glutelin,dry and wet gluten and SDS-sedimentation value of wheat grain showed a downward trend with the increase of the germination time of wheat grains. Among them,T2 was decreased by 16.15%,19.25%,3.17%,5.95%,6.86%,9.58%,5.36% and 12.53% respectively at 84 hours,in which the reduction percentage significantly was less than CK,but the amylose did not show the significant effect. The contents of albumin and globulin showed an increasing trend,and the increase percentage of albumin was greater than that of globulin. However,the contents of gliadin and glutenin decreased with the increase of germination time,T2 and M4 were significantly different with CK at 84 h,and glutenin was more affected by germination.[Conclusions] The application of 1 500 mg·L-1 biological retardant can significantly delay the pre-harvest sprouting of wheat and ensure the vigor of wheat seeds,and alleviate the negative effects of the pre-harvest sprouting of wheat,which resulted in the decrease of yield and quality.

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备注/Memo

备注/Memo:
收稿日期:2020-04-08。
基金项目:国家重点研发计划项目(2017YFD0201300)
作者简介:毛志强,助理工程师,硕士,主要研究方向为作物生理生态和生长调控,E-mail:maozhiqiang@sinochem.com。
通信作者:周繁,高级工程师,博士,研究方向为作物生长调控与功能微肥研发,E-mail:zhoufan03@sinochem.com。
更新日期/Last Update: 1900-01-01