[1]姚宏,郑玉才,李志雄,等.低氧环境下蛋内注射CuSO4对藏鸡和肉鸡鸡胚发育的影响[J].南京农业大学学报,2020,43(6):1079-1086.[doi:10.7685/jnau.201910038]
 YAO Hong,ZHENG Yucai,LI Zhixiong,et al.Effects of in ovo injection of CuSO4 on the development of Tibetan chicken and broiler chicken embryos hatched under hypoxia conditions[J].Journal of Nanjing Agricultural University,2020,43(6):1079-1086.[doi:10.7685/jnau.201910038]
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低氧环境下蛋内注射CuSO4对藏鸡和肉鸡鸡胚发育的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
43卷
期数:
2020年6期
页码:
1079-1086
栏目:
动物科学
出版日期:
2020-11-10

文章信息/Info

Title:
Effects of in ovo injection of CuSO4 on the development of Tibetan chicken and broiler chicken embryos hatched under hypoxia conditions
作者:
姚宏1 郑玉才1 李志雄1 王云浩1 谈永萍1 徐亚欧1 王爱林1 董传豪1 冯卫东2 饶开晴1
1. 西南民族大学生命科学与技术学院, 四川 成都 610041;
2. 甘孜州畜牧业科学研究所, 四川 康定 626000
Author(s):
YAO Hong1 ZHENG Yucai1 LI Zhixiong1 WANG Yunhao1 TAN Yongping1 XU Ya’ou1 WANG Ailin1 DONG Chuanhao1 FENG Weidong2 RAO Kaiqing1
1. College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China;
2. Ganzi Prefectural Livestock Research Institute, Kangding 626000, China
关键词:
低氧蛋内注射铜离子血管生成抗氧化
Keywords:
hypoxiain ovo injectioncopper ionangiogenesisantioxidation
分类号:
S831.1
DOI:
10.7685/jnau.201910038
摘要:
[目的] 本文旨在研究低氧环境下改变种蛋内铜离子(Cu2+)含量对藏鸡、科宝肉鸡两品种鸡胚发育、血管生成和机体抗氧化性能的影响及机制。[方法] 选择质量相近的藏鸡、科宝肉鸡种蛋各132枚,随机分为3组。孵化前蛋内注射不同Cu2+浓度的CuSO4注射液100 μL:对照组(仅注射100 μL 90 μg·mL-1 NaCl溶液)、低剂量组(75 μg·mL-1 Cu2+),高剂量组(300 μg·mL-1 Cu2+),低氧环境下孵化。12胚龄时固定和采集尿囊膜,采用Image-Pro Plus 6.0图像分析软件对鸡胚尿囊膜血管面积、条数进行测量;记录出雏率,采集1日龄血清及各组织并测定其质量;使用试剂盒检测与抗氧化相关的指标;采用RT-qPCR检测肝脏和尿囊膜中血管内皮生长因子(VEGF)基因的表达。[结果] 肉鸡出雏率随蛋内注射Cu2+浓度增加而升高;与对照组相比,低剂量组肉鸡出雏质量/蛋质量、腿体比以及高剂量组肝体比均显著提高(P<0.05),藏鸡低剂量组肝体比显著升高(P<0.05)。与对照组相比,肉鸡高剂量组的尿囊膜血管面积和中血管数显著增加(P<0.05),高剂量组的藏鸡尿囊膜上大、中、小血管形成以及血管面积增加(P<0.05),低剂量组的藏鸡尿囊膜中、小血管数量显著增加(P<0.05)。藏鸡、肉鸡试验组血清中铜锌超氧化物歧化酶(Cu/Zn-SOD)活性均显著高于对照组(P<0.05),丙二醛(MDA)含量显著低于对照组(P<0.05),而谷胱甘肽过氧化物酶(GSH-Px)活性不受Cu2+的影响。注射高剂量的CuSO4能显著上调藏鸡、肉鸡肝脏和尿囊膜中VEGF基因的表达量(P<0.05),且藏鸡尿囊膜和肝脏中VEGF基因的表达量均显著高于肉鸡(P<0.05)。[结论] 低氧环境下种蛋内注射CuSO4可提高藏鸡和肉鸡机体的抗氧化能力,促进两品种鸡胚血管生成以应对低氧环境;肉鸡机体抗氧化能力强于藏鸡,而藏鸡的血管生成能力强于肉鸡。
Abstract:
[Objectives] The present experiment was conducted to study the effects of changing the copper ion content on embryonic development,angiogenesis and anti-oxidant performance of Tibetan chickens and Cobb broilers under hypoxic conditions,and to explore the mechanism of these effects.[Methods] Each of 132 fertilized eggs with similar quality of Tibetan chickens and Cobb broilers were selected and randomly allocated into three groups. 100 μL of CuSO4 injection with different Cu2+ concentrations were in ovo injection before incubation. 90 μg·mL-1 NaCl solution in the control group,75 μg·mL-1 Cu2+ in the low-dose group,and 300 μg·mL-1 Cu2+ in the high-dose group were hatched under hypoxia conditions. Chorioallantoic membrane(CAM) was fixed and collected on 12 embryonic age(E12). Image-Pro Plus 6.0 image analysis software was used to measure the blood vessel area and number of strips in CAM on E12;the hatching rate was observed at hatching,and each tissue sample was collected and measured on the quality on one-day-old;the anti-oxidation related indexes were detected using the kit;the expression of VEGF gene in liver and CAM was detected by RT-qPCR.[Results] The hatching rate of broiler chickens enhanced with the increase concentration of Cu2+ injected into the eggs. Compared with the control group,the hatching weight/egg weight,leg-body ratio of the low dose group and liver-body ratio of the high dose group significantly increased(P<0.05). The liver-body ratio of Tibetan chickens in the low-dose group was significantly higher than that of the control group(P<0.05). The vascular area and the number of middle blood vessels in CAM were significantly increased in broiler chicken group with high dose(P<0.05). The high dose of copper ion could promote the formation of large,medium and small blood vessels and increase the vascular area in CAM of Tibetan chickens(P<0.05). The number of small and medium blood vessels in CAM of Tibetan chickens in the low-dose group significantly increased(P<0.05). The activity of Cu,Zn-superoxide dismutase(Cu/Zn-SOD) in the serum of Tibetan chickens and broiler chickens experimental group was significantly higher than that of the control group(P<0.05),and malondialdehyde(MDA) content was significantly lower than those in the control group(P<0.05). However,glutathione peroxidase(GSH-Px) activity was not affected by copper. Injection of high dose CuSO4 could significantly up-regulate the expression of VEGF gene in the liver and CAM of Tibetan chickens and broiler chickens(P<0.05),and the expression of VEGF gene in the CAM and liver of Tibetan chickens was higher than that of broiler chickens(P<0.05).[Conclusions] In ovo injection of CuSO4 in hypoxic environment could improve the anti-oxidant performance of the body of Tibetan chickens and broiler chickens,and promote the angiogenesis of embryo of two breeds of chicken to cope with the hypoxia environment;the anti-oxidant performance of broiler chickens was stronger than that of Tibetan chickens,and the angiogenesis capacity of Tibetan chickens was stronger than that of broiler chickens.

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备注/Memo

备注/Memo:
收稿日期:2019-10-24。
基金项目:四川省科技支撑计划"十三五育种攻关"项目(2016NYZ0043)
作者简介:姚宏,硕士研究生。
通信作者:饶开晴,副教授,博士,研究方向为动物生长调控,E-mail:wkxwzz6@163.com。
更新日期/Last Update: 1900-01-01