[1]贾兵,余桃,郭国凌,等.‘砀山酥梨’宿萼果与脱萼果内源激素含量比较及其信号传导基因的表达差异[J].南京农业大学学报,2021,44(3):428-436.[doi:10.7685/jnau.202008002]
 JIA Bing,YU Tao,GUO Guoling,et al.Comparision of endogenous hormones content and expression difference of signal transduction genes between the calyx-persistence fruit and calyx-shedding fruit in ‘Dangshansuli’pear[J].Journal of Nanjing Agricultural University,2021,44(3):428-436.[doi:10.7685/jnau.202008002]
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‘砀山酥梨’宿萼果与脱萼果内源激素含量比较及其信号传导基因的表达差异()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
44卷
期数:
2021年3期
页码:
428-436
栏目:
植物科学
出版日期:
2021-05-10

文章信息/Info

Title:
Comparision of endogenous hormones content and expression difference of signal transduction genes between the calyx-persistence fruit and calyx-shedding fruit in ‘Dangshansuli’pear
作者:
贾兵 余桃 郭国凌 王友煜 许波 刘普 衡伟 朱立武
安徽农业大学园艺学院, 安徽 合肥 230036
Author(s):
JIA Bing YU Tao GUO Guoling WANG Youyu XU Bo LIU Pu HENG Wei ZHU Liwu
College of Horticulture, Anhui Agricultural University, Hefei 230036, China
关键词:
砀山酥梨宿萼果脱萼果内源激素信号传导基因
Keywords:
Dangshansulicalyx-persistence fruitcalyx-shedding fruitendogenous hormonessignal transduction genes
分类号:
S661.2
DOI:
10.7685/jnau.202008002
摘要:
[目的] 本文旨在探明‘砀山酥梨’宿萼果与脱萼果内源激素含量及其信号传导基因表达的差异。[方法] 以‘砀山酥梨’为试材,在盛花期喷施400 mg·L-1赤霉素(GA3)、200 mg·L-1多效唑(PP333)溶液,于盛花后10 d,分别取对照及外源生长调节剂处理的宿萼果和脱萼果,测定内源激素生长素(IAA)、玉米素(ZR)、GA3和脱落酸(ABA)的含量。从NCBI梨基因组数据库中查询内源激素相关信号传导基因,并运用RT-qPCR技术,分析其在宿萼果与脱萼果的相对表达量。[结果] ‘砀山酥梨’宿萼果中IAA和GA3含量显著高于脱萼果,而ZR和ABA含量无显著差异。宿萼果中高含量的IAA促使膜转运蛋白基因pbAUX1和核内受体蛋白基因pbTIR1高表达,生长素抑制基因pbAUX/IAA1、pbAUX/IAA2表达量显著下降,生长素响应因子pbARF1、pbARF2表达量显著上升,激活了原初反应基因pbGH3.6、pbSAUR32,调控宿萼果的发育。宿萼果中CTK膜转运蛋白基因pbCRE1/2/3/4和核内反应调节子pbARR1/11表达量均显著高于脱萼果。宿萼果核内GA受体蛋白基因pbGID1表达量显著高于脱萼果,诱导其泛素化酶体蛋白基因pbGID2和转录因子基因pbTF5的表达,参与调控宿萼果的发育。宿萼果中ABA信号传导基因pbPP2C-2、pbPP2C-50、pbSnRK2-2pbABF-5-5表达量显著高于脱萼果。[结论] ‘砀山酥梨’萼片的宿存主要受幼果内IAA、GA含量及其信号传导基因调控,且幼果内高含量的IAA、GA3有利于萼片宿存。
Abstract:
[Objectives] The paper aimed to explore the difference of endogenous hormone content and expression difference of signal transduction genes between the calyx-persistence fruit(CPF) and calyx-shedding fruit(CSF) in ‘Dangshansuli’ pear. [Methods] ‘Dangshansuli’ pear was used as experimental material,and 400 mg·L-1 GA3 and 200 mg·L-1PP333 solution was sprayed at full bloom stage,respectively. The CPF and CSF under natural conditions,treated with GA3 and PP333 were respectively taken at 10 days after full bloom to determine the auxin(IAA),zeatin(ZR),GA3 and abscisic acid(ABA) contents. Furthermore,related signaling genes of those endogenous hormones were identified by referring to pear genome database in NCBI. What’s more,RT-qPCR was used to analyze their relative expression in CPF and CSF. [Results] IAA and GA3 contents in the CPF were significantly higher than those in the CSF while ZR and ABA contents showed no marked differences. High IAA contents in the CPF notably prompted the expression of membrane transporter gene pbAUX1 and nuclear receptor protein gene pbTIR1,significantly decreased the expression level of the auxin inhibitor genes pbAUX/IAA1,pbAUX/IAA2,and significantly increased the expression level of auxin response factor genes pbARF1 and pbARF2,which then activated the expression of the primary response gene pbGH3.6 and pbSAUR32,thus regulating the development of CPF. The expression levels of membrane transporter gene pbCRE1/2/3/4 and intranuclear reaction regulator pbARR1/11 in the CPF was significantly higher than those in the CSF. The expression level of receptor protein gene pbGID1 was also significantly higher than that in the CSF,and the expression of ubiquitinase protein gene pbGID2 and transcription factor pbTF5 were induced in the CPF,which were involved in the regulation of the CPF development. Similarly,the expression levels of ABA signaling genes pbPP2C-2,pbPP2C-50,pbSnRK2-2 and pbABF-5-5 remarkably increased in CPF. [Conclusions] Therefore,the calyx persistence of ‘Dangshansuli’ pear were closely modulated by IAA,GA contents and the related signaling genes expression. Moreover,high concentration of IAA in young fruit was favorable for it.

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备注/Memo

备注/Memo:
收稿日期:2020-08-03。
基金项目:国家现代农业产业技术体系建设专项(CARS-29-14)
作者简介:贾兵,副教授,主要从事梨栽培生理研究,E-mail:jb1977@ahau.edu.cn。
更新日期/Last Update: 1900-01-01