[1]马稳霞,冯升来,涂艺馨,等.黄花蒿精油及其活性成分1,8-cineole对兰州百合采后病害的体外防治[J].南京农业大学学报,2021,44(3):447-456.[doi:10.7685/jnau.202010027]
 MA Wenxia,FENG Shenglai,TU Yixin,et al.In vitro prevention and cure of essential oil from Artemisia annua L. and its active ingredient 1,8-cineole on postharvest diseases of Lilium davidii var. unicolor[J].Journal of Nanjing Agricultural University,2021,44(3):447-456.[doi:10.7685/jnau.202010027]
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黄花蒿精油及其活性成分1,8-cineole对兰州百合采后病害的体外防治()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
44卷
期数:
2021年3期
页码:
447-456
栏目:
植物科学
出版日期:
2021-05-10

文章信息/Info

Title:
In vitro prevention and cure of essential oil from Artemisia annua L. and its active ingredient 1,8-cineole on postharvest diseases of Lilium davidii var. unicolor
作者:
马稳霞 冯升来 涂艺馨 令利军
西北师范大学生命科学学院/甘肃特色植物生物活性产品工程研究中心, 甘肃 兰州 730070
Author(s):
MA Wenxia FENG Shenglai TU Yixin LING Lijun
College of Life Science/Bioactive Products Engineering Research Center for Gansu Distinctive Plants, Northwest Normal University, Lanzhou 730070, China
关键词:
兰州百合致病菌鉴定精油18-cineole抗菌活性
Keywords:
Lilium davidii var. unicolorpathogenic microorganismidentificationessential oil18-cineoleantimicrobial activity
分类号:
S432.1
DOI:
10.7685/jnau.202010027
摘要:
[目的] 利用黄花蒿精油及其活性成分1,8-cineole对兰州百合采后病害致病菌进行体外防治,为兰州百合采后病害的生物防治提供理论基础。[方法] 采用组织分离法及柯赫氏回接法分离兰州百合采后病害致病菌并确定致病性,结合形态学、分子生物学进行鉴定确定其分类地位,并通过熏蒸法和直接接触法检测黄花蒿精油及其活性成分1,8-cineole的体外抑菌活性。[结果] 致伤情况下引起兰州百合采后病害的病原菌包括1株细菌[摩加夫芽胞杆菌(Bacillus mojavensis)B-6]和3株丝状真菌[李氏木霉(Trichoderma lixii)F-2、篮状菌(Talaromyces tumuli)F-3和镰刀菌(Fusarium annulatum)F-6];无致伤情况下,菌株B-6对兰州百合无侵染现象,菌株F-2、F-3及F-6侵染速度相比致伤较慢。熏蒸法结果显示:黄花蒿精油及1,8-cineole的浓度达到0.7 mL·L-1时,对B-6的杀菌率达到100%,对病原真菌的EC50范围分别为0.314~0.357 mL·L-1和0.280~0.420 mL·L-1。直接接触法结果显示:黄花蒿精油及1,8-cineole对B-6的最低杀菌浓度分别为1.25和5 mL·L-1,黄花蒿精油对F-2、F-3及F-6的杀菌浓度分别为10、40和40 mL·L-1,1,8-cineole对F-2、F-3及F-6的杀菌浓度均为20 mL·L-1[结论] 从腐烂的兰州百合中分离得到的4株致病菌均能不同程度地引起兰州百合采后病害,且致伤能够促进病原菌F-2、F-3及F-6对兰州百合的侵染速度;黄花蒿精油及其主要活性成分1,8-cineole均对兰州百合采后病害致病菌具有良好的抑菌活性,熏蒸法更具优势。
Abstract:
[Objectives] The essential oil from Artemisia annua and its active ingredient 1,8-cineole were used for the in vitro control of postharvest diseases of Lilium davidii,providing a theoretical basis for biological control of postharvest diseases of L.davidii. [Methods] Pathogenicity of postharvest diseases of L.davidii was isolated by tissue separation method and Koch tieback method,and its classification status was determined by combining morphology and molecular biology. In vitro antibacterial activity of essential oil from A.annua and its active component 1,8-cineole were determined by fumigation method and direct contact method. [Results] In the case of injury,the pathogens causing postharvest diseases of L.davidii included 1 strain of Bacillus mojavensis(B-6) and three strains of fungi[Trichoderma lixii(F-2),Talaromyces tumuli(F-3) and Fusarium annulatum(F-6)]; without injury,strain B-6 had no infection on L.davidii,and the infection rate of strains F-2,F-3 and F-6 was slower than that of injury. The fumigation method showed when the concentration of essential oil from A.annua and 1,8-cineole reached 0.7 mL·L-1,the bactericidal rate of B-6 reached 100%,and the EC50 ranges against pathogenic fungi were 0.314-0.357 mL·L-1 and 0.280-0.420 mL·L-1,respectively. The result of direct contact method showed the minimum bactericidal concentrations of essential oil of A.annua and 1,8-cineole to B-6 were 1.25 mL·L-1 and 5 mL·L-1,and the concentrations of essential oil from A.annua of fungicidal to F-2,F-3 and F-6 were 10 mL·L-1,40 mL·L-1 and 40 mL·L-1,respectively,and the concentrations of 1,8-cineole of fungicidal to F-2,F-3 and F-6 were all 20 mL·L-1. [Conclusions] It is found that the 4 pathogens isolated from the rotten L.davidii could all cause the postharvest diseases of L.davidii to varying degrees,and the injury promotes the infection rate of L.davidii by pathogenic of F-2,F-3 and F-6. The essential oil of A.annua and its main active ingredient 1,8-cineole have good antimicrobial activity,and the fumigation method is more advantageous.

参考文献/References:

[1] 杨雨华. 半干旱地区兰州百合对地膜覆盖和施肥的生态学效应研究[D]. 兰州:兰州大学,2011. Yang Y H. Research on ecological effect of Lilium davidii var. unicolor under film mulches and fertilization[D]. Lanzhou:Lanzhou University,2011(in Chinese with English abstract).
[2] Ling L J,Jiao Z L,Ma W X,et al. Preliminary report on the study of postharvest fruit rot bacteria and yeasts in Lanzhou lily(Lilium davidii var. unicolor) in China[J]. Journal of Phytopathology,2019,167(3):135-145.
[3] 赵银彦,谢忠奎,胡秉安,等. 兰州百合生产现状与效益分析[J]. 中国蔬菜,2018(2):71-75. Zhao Y Y,Xie Z K,Hu B A,et al. Production status and benefit analysis of Lanzhou lily[J]. China Vegetables,2018(2):71-75(in Chinese with English abstract).
[4] 赵丽君.临洮县百合产业发展现状及对策[J]. 农业科技与信息,2019(11):62-63. Zhao L J. Development status and countermeasures of lily industry in Lintao County[J]. Agricultural Science and Technology and Information,2019(11):62-63(in Chinese).
[5] Janisiewicz W J,Conway W S. Combining biological control with physical and chemical treatments to control fruit decay after harvest[J]. Stewart Postharvest Review,2010,6(1):1-16.
[6] 巩慧玲,孙爱洁,李茜,等. 兰州百合鳞茎贮藏腐烂病原菌的鉴定及生物学特性[J]. 食品工业科技,2015,36(20):97-101,106. Gong H L,Sun A J,Li X,et al. Identification of the pathogen from stored Lanzhou lily bulb rot disease and biological characteristics[J]. Science and Technology of Food Industry,2015,36(20):97-101,106(in Chinese with English abstract).
[7] Malik A A,Ahmad J,Mir S R,et al. Influence of chemical and biological treatments on volatile oil composition of Artemisia annua Linn[J]. Industrial Crops & Products,2009,30(3):380-383.
[8] Juergens L J,Worth H,Juergens U R. New perspectives for mucolytic,anti-inflammatory and adjunctive therapy with 1,8-cineole in COPD and asthma:review on the new therapeutic approach[J]. Advances in Therapy,2020,37(5):1737-1753.
[9] Mollaei S,Khanehbarndaz O,Gerami-Khashal Z,et al. Molecular identification and phytochemical screening of endophytic fungi isolated from Lithospermum officinale L. roots:a new source of shikonin[J]. Phytochemistry,2019,168:112116.
[10] 刘瑞玲,郜海燕,陈杭君,等. 红肉火龙果采后病原菌分离鉴定与植物精油抑菌研究[J]. 农业机械学报,2018,49(8):338-345. Liu R L,Gao H Y,Chen H J,et al. Isolation,identification and inhibition of pathogens from red pitaya fruit during storage[J]. Transactions of the Chinese Society for Agricultural Machinery,2018,49(8):338-345(in Chinese with English abstract).
[11] Dede A,Guven K,Sahin N. Isolation,plant growth-promoting traits,antagonistic effects on clinical and plant pathogenic organisms and identification of actinomycetes from olive rhizosphere[J]. Microbial Pathogenesis,2020,143:104134.
[12] Uwalaka E I,Kassim Z J,Abdulmumin A R,et al. Isolation and identification of antibiotic producing bacteria from soil samples of abattoir in Lapai,Niger state,Nigeria[J]. Journal of Applied Life Sciences International,2019,22(2):1-8.
[13] 苏静,李双明. 土传病原真菌及其生防细菌的分离鉴定[J]. 草原与草坪,2019,39(3):79-84,91. Su J,Li S M. Isolation and identification of soil-borne pathogenic fungi and biocontrol bacteria[J]. Grassland and Turf,2019,39(3):79-84,91(in Chinese with English abstract).
[14] Vettraino A M,Natili G,Anselmi N,et al. Recovery and pathogenicity of Phytophthora species associated with a resurgence of ink disease in Castanea sativa in Italy[J]. Plant Pathology,2001,50(1):90-96.
[15] 夏乐先,孙文娟,沈振,等. 煮沸裂解法和试剂盒法提取浸矿菌基因组DNA的比较[J]. 现代生物医学进展,2014,14(1):31-35,17. Xia L X,Sun W J,Shen Z,et al. Comparison of genomic DNA extraction from bioleaching acidophiles by two methods:boiling and kit methods[J]. Progress in Modern Biomedicine,2014,14(1):31-35,17(in Chinese with English abstract).
[16] Hatamzadeh S,Rahnama K,Nasrollahnejad S,et al. Isolation and identification of L-asparaginase-producing endophytic fungi from the Asteraceae family plant species of Iran[J]. PeerJ,2020,8:e8309.
[17] Majeed H A,Seger Q J. Isolation and identification of two fungal colonies exhibiting antagonistic growth[J]. Journal of Forensic Medicine,2019,13(4):303-307.
[18] Mehraban A,Nasr R,Eslami M,et al. Molecular and morphological identification of fungi isolated from sour cherry,plum and apple fruit leather in Iran[J]. Gene Reports,2019,17:100500.
[19] K?ralan S S,Toptanc??,?ncül Abac?gil T,et al. Phthalates levels in olive oils and olive pomace oils marketed in Turkey[J]. Food Additives & Contaminants:Part A,2020,37(8):1332-1338.
[20] 施俊凤,王潇冉,孙常青,等. 肉豆蔻精油对番茄采后病原真菌的抗菌活性研究[J]. 保鲜与加工,2013,13(1):28-32. Shi J F,Wang X R,Sun C Q,et al. Studies on antifungal activities of myristica oil against pathogenic fungal strains on postharvest tomato[J]. Storage and Process,2013,13(1):28-32(in Chinese with English abstract).
[21] Béjaoui A,Chaabane H,Jemli M,et al. Essential oil composition and antibacterial activity of Origanum vulgare subsp. glandulosum Desf. at different phenological stages[J]. Journal of Medicinal Food,2013,16(12):1115-1120.
[22] 冯中红,畅涛,杨成德,等. 一株抗马铃薯坏疽病莫海威芽孢杆菌(Bacillus mojavensis ZA1)培养条件的优化[J]. 草业学报,2016,25(2):77-86. Feng Z H,Chang T,Yang C D,et al. Optimization of culture conditions of a biocontrol agent Bacillus mojavensis ZA1 to antagonize Phoma foveata[J]. Acta Prataculturae Sinica,2016,25(2):77-86(in Chinese with English abstract).
[23] From C,Hormazabal V,Hardy S P,et al. Cytotoxicity in Bacillus mojavensis is abolished following loss of surfactin synthesis:implications for assessment of toxicity and food poisoning potential[J]. International Journal of Food Microbiology,2007,117(1):43-49.
[24] 金鑫,李孝敬,黄艺伟,等. 不同年份三七根际土壤中真菌的分离鉴定及其多样性的比较[J]. 中药材,2020,43(2):314-317. Jin X,Li X J,Huang Y W,et al. Isolation,identification and diversity comparison of rhizosphere fungi of panax notoginseng in different years[J]. Journal of Chinese Medicinal Materials,2020,43(2):314-317(in Chinese).
[25] 刘小燕,高智谋,汪世军,等. 油菜菌核病菌致病性分化及其与纤维素酶活性的关系[C]//中国植物病理学会2009年学术年会论文集. 昆明,2009:33-34. Liu X Y,Gao Z M,Wang S J,et al. Pathogenicity differentiation and its ralationship to cellulase activity of Sclerotinia sclerotiorum isolates[C]//Proceedings of the 2009 Annual Conference of the Chinese Society of Plant Pathology,Kunming,2009:33-34(in Chinese).
[26] 张志元,官春云. 不同栽培方式对辣椒采后病害的影响[J]. 植物病理学报,2003,33(2):183-186. Zhang Z Y,Guan C Y. Effects of different cultivation methods on the pepper postharvest disease[J]. Acta Phytopathologica Sinica,2003,33(2):183-186(c with English abstract).
[27] 王书平,吴涛,陆玉建.几种芳香植物精油的抑菌活性研究[J]. 安徽农学通报,2018,24(2):20-24. Wang S P,Wu T,Lu Y J. Study on antimicrobial activity of several kinds of aromatic essential oil[J]. Anhui Agricultural Science Bulletin,2018,24(2):20-24(in Chinese with English abstract).
[28] 高志玲,陈艳,谢英辉. 黄花蒿挥发油的体外抑菌活性研究[J]. 食品科学,2010,31(19):209-211. Gao Z L,Chen Y,Xie Y H. In vitro antimicrobial activity of essential oil from Artemisia annua[J]. Food Science,2010,31(19):209-211(in Chinese with English abstract).
[29] 夏明静,曹煜,杨捷,等. 22种抗菌消炎中药有效成分对痤疮丙酸杆菌的抑制作用[J]. 中华皮肤科杂志,2001,34(6):435-436. Xia M J,Cao Y,Yang J,et al. Inhibition of Propionibacterium acnes by effective components of traditional Chinese medicines[J]. Chinese Journal of Dermatology,2001,34(6):435-436(in Chinese with English abstract).
[30] Huang J H,Qian C,Xu H J,et al. Antibacterial activity of Artemisia asiatica essential oil against some common respiratory infection causing bacterial strains and its mechanism of action in Haemophilus influenzae[J]. Microbial Pathogenesis,2018,114:470-475.

备注/Memo

备注/Memo:
收稿日期:2020-10-22。
基金项目:兰州市科技计划项目(2018-1-104);甘肃省国际科技合作专项(1504WKCA028);甘肃省引导科技创新发展专项资金(2019ZX-05)
作者简介:马稳霞,硕士研究生。
通信作者:令利军,副教授,研究方向为植物与微生物互作,E-mail:13919343210@163.com。
更新日期/Last Update: 1900-01-01