[1]陈淋,王兴红,刘云鹏.增强贝莱斯芽胞杆菌CLA178根际定殖的蔷薇根系分泌组分鉴定[J].南京农业大学学报,2021,44(3):497-505.[doi:10.7685/jnau.202008004]
 CHEN Lin,WANG Xinghong,LIU Yunpeng.Root exudates in Rosa-microbe interaction contribute to the increased colonization of Bacillus velezensis CLA178[J].Journal of Nanjing Agricultural University,2021,44(3):497-505.[doi:10.7685/jnau.202008004]
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增强贝莱斯芽胞杆菌CLA178根际定殖的蔷薇根系分泌组分鉴定()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
44卷
期数:
2021年3期
页码:
497-505
栏目:
生物与环境
出版日期:
2021-05-10

文章信息/Info

Title:
Root exudates in Rosa-microbe interaction contribute to the increased colonization of Bacillus velezensis CLA178
作者:
陈淋1 王兴红1 刘云鹏2
1. 中国林业科学研究院华北林业实验中心, 北京 102300;
2. 中国农业科学院农业资源与农业区化研究所, 北京 100081
Author(s):
CHEN Lin1 WANG Xinghong1 LIU Yunpeng2
1. Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, China;
2. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
关键词:
蔷薇根系分泌物贝莱斯芽胞杆菌趋化生物膜根际定殖
Keywords:
roseroot exudatesBacillus velezensischemotaxisbiofilmroot colonization
分类号:
S182
DOI:
10.7685/jnau.202008004
摘要:
[目的] 根系分泌物在植物-微生物根际互作过程中起到关键作用,本文旨在深入了解根际促生菌与蔷薇植物之间的根际互作机制。[方法] 通过分根系统检测预接种益生菌或病原菌对植物根系分泌物组分和根际促生菌贝莱斯芽胞杆菌(Bacillus velezensis) CLA178菌株定殖的影响;通过分析不同处理的蔷薇根际分泌物组分与菌株定殖的相关性,筛选潜在的信号物质;通过检测不同物质与CLA178菌株趋化性、生物膜形成、生长、定殖及相关基因表达的关系,确定调控菌株CLA178定殖的根际信号物质。[结果] 预接种CLA178或根癌农杆菌(Agrobacterium tumefaciens) C58均会促进CLA178定殖。根系分泌物中的葡萄糖酸(GLcA)和海藻糖-6-磷酸(T6P)的含量与CLA178的趋化性、生物膜形成、根际定殖和epsD、yqxM、kinC基因表达均呈正相关关系,20~100 μmol·L-1 GLcA和T6P分别显著提高CLA178的根际定殖;而CLA178对邻苯二甲酸二辛酯(DOP)无显著趋化响应,但是该物质与CLA178的生长、生物膜形成、定殖呈负相关。[结论] 预接种贝莱斯芽胞杆菌CLA178和根癌农杆菌C58后,蔷薇根系分泌物中GLcA和T6P含量增加,DOP含量降低,参与互作过程中益生菌CLA178根际定殖增强,进而协助植物防控病害。
Abstract:
[Objectives] Root exudates play an important role in plant-microbe rhizosphere interactions. This paper was aimed to further understand the rose root exudates-mediated interaction between rose and plant growth-promoting rhizobacteria(PGPR). [Methods] The impacts of preinoculation with beneficial bacteria or pathogens on root exudates and subsequent colonization by PGPR Bacillus velezensis CLA178 were investigated in a split-root system. The potential signal compounds were selected through analyzing the correlation between root exudates and root colonization by CLA178. The signal compounds in root exudates were determined by measuring the effects of different compounds on the chemotaxis,biofilm formation,growth,colonization and related genes expression of CLA178. [Results] Preinoculation of rose plants in a split-root system with B. velezensis CLA178 or pathogen Agrobacterium tumefaciens C58 inhibited the subsequent colonization by CLA178. The root secretion of gluconic acid(GLcA) and trehalose-6-phosphate(T6P) had positive effects on the chemotaxis,biofilm formation,colonization and expression of epsD,yqxM,and kinC genes of CLA178. The colonization of CLA178 was significantly enhanced by 20-100 μmol·L-1 GLcA and T6P,respectively. The root secretion of dioctyl phthalate(DOP) had no significant effect on the chemotaxis of CLA178,but had negative effects on the growth,biofilm formation and colonization of CLA178. [Conclusions] Rose plants enhanced the root secretion of GLcA and T6P,and reduced the root secretion of DOP in response to microbial colonization,thereby increasing the colonization of B. velezensis CLA178 to assist plant in disease resistance.

参考文献/References:

[1] Gohlke J,Deeken R. Plant responses to Agrobacterium tumefaciens and crown gall development[J]. Frontiers in Plant Science,2014,5:1-11.
[2] Kawaguchi A,Inoue K,Ichinose Y. Biological control of crown gall of grapevine,rose,and tomato by nonpathogenic Agrobacterium vitis strain VAR03-1[J]. Phytopathology,2008,98(11):1218-1225.
[3] Waard M A,Georgopoulos S G,Hollomon D W,et al. Chemical control of plant diseases:problems and prospects[J]. Annual Review of Phytopathology,1993,31(1):403-421.
[4] Dong S J,Tredway L P,Shew H D,et al. Resistance of transgenic tall fescue to two major fungal diseases[J]. Plant Science,2007,173(5):501-509.
[5] Xu Z H,Zhang R F,Wang D D,et al. Enhanced control of cucumber wilt disease by Bacillus amyloliquefaciens SQR9 by altering the regulation of its DegU phosphorylation[J]. Applied and Environmental Microbiology,2014,80(9):2941-2950.
[6] Stringlis I A,Proietti S,Hickman R,et al. Root transcriptional dynamics induced by beneficial rhizobacteria and microbial immune elicitors reveal signatures of adaptation to mutualists[J]. The Plant Journal,2018,93(1):166-180.
[7] Pascale A,Proietti S,Pantelides I S,et al. Modulation of the root microbiome by plant molecules:the basis for targeted disease suppression and plant growth promotion[J]. Frontiers in Plant Science,2020,10:1741.
[8] Venturi V,Keel C. Signaling in the rhizosphere[J]. Trends in Plant Science,2016,21(3):187-198.
[9] Rasmann S,Turlings T C. Root signals that mediate mutualistic interactions in the rhizosphere[J]. Current Opinion in Plant Biology,2016,32:62-68.
[10] Badri D V,Vivanco J M. Regulation and function of root exudates[J]. Plant,Cell & Environment,2009,32(6):666-681.
[11] Bais H P,Weir T L,Perry L G,et al. The role of root exudates in rhizosphere interactions with plants and other organisms[J]. Annual Review of Plant Biology,2006,57:233-266.
[12] Hu L F,Robert C A M,Cadot S,et al. Root exudate metabolites drive plant-soil feedbacks on growth and defense by shaping the rhizosphere microbiota[J]. Nature Communications,2018,9:2738.
[13] Berendsen R L,Vismans G,Yu K,et al. Disease-induced assemblage of a plant-beneficial bacterial consortium[J]. The ISME Journal,2018,12(6):1496-1507.
[14] Liu Y P,Chen L,Wu G W,et al. Identification of root-secreted compounds involved in the communication between cucumber,the beneficial Bacillus amyloliquefaciens,and the soil-borne pathogen Fusarium oxysporum[J]. Molecular Plant-Microbe Interactions,2017,30(1):53-62.
[15] Murashige T,Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures[J]. Physiologia Plantarum,1962,15(3):473-497.
[16] Shen C H,Xu P,Huang Z,et al. Bacterial chemotaxis on SlipChip[J]. Lab Chip,2014,14(16):3074-3080.
[17] Chen L,Ma Q H,Liu H H,et al. Reduced root secretion of valine in Rosa-microbe interaction contributes to the decreased colonization of pathogenic Agrobacterium tumefaciens[J]. Plant Disease,2021:PDIS-06-20-1179.
[18] Lugtenberg B,Kamilova F. Plant-growth-promoting rhizobacteria[J]. Annual Review of Microbiology,2009,63:541-556.
[19] Mauck K E,de Moraes C M,Mescher M C. Deceptive chemical signals induced by a plant virus attract insect vectors to inferior hosts[J]. Proc Natl Acad Sci USA,2010,107(8):3600-3605.
[20] 罗兴,冯海超,夏丽明,等. 根际促生解淀粉芽胞杆菌SQR9对香蕉根系分泌物响应的转录组分析[J]. 南京农业大学学报,2019,42(1):102-110. DOI:10.7685/jnau.201804025. Luo X,Feng H C,Xia L M,et al. Transcriptomic profiling of plant growth-promoting rhizobacteria Bacillus amyloliquefaciens SQR9 in response to banana root exudates[J]. Journal of Nanjing Agricultural University,2019,42(1):102-110(in Chinese with English abstract).
[21] 董丽红,郭庆港,张晓云,等. 棉花根系分泌物对枯草芽胞杆菌NCD-2菌株趋化性的影响[J]. 植物病理学报,2019,49(3):399-407. Dong L H,Guo Q G,Zhang X Y,et al. Effect of cotton root exudates on the chemotaxis of Bacillus subtilis strain NCD-2[J]. Acta Phytopathologica Sinica,2019,49(3):399-407(in Chinese with English abstract).
[22] 沈怡斐,鄂垚瑶,阳芳,等. 西瓜根系分泌物中氨基酸组分对多黏类芽胞杆菌SQR-21趋化性及根际定殖的影响[J]. 南京农业大学学报,2017,40(1):101-108. DOI:10.7685/jnau.201603051. Shen Y F,E Y Y,Yang F,et al. Effects of amino acids in root exudates of watermelon on the chemotactic reaction and root colonization of Paenibacillus polymyxa SQR-21[J]. Journal of Nanjing Agricultural University,2017,40(1):101-108(in Chinese with English abstract).
[23] Feng H C,Zhang N,Du W B,et al. Identification of chemotaxis compounds in root exudates and their sensing chemoreceptors in plant-growth-promoting rhizobacteria Bacillus amyloliquefaciens SQR9[J]. Molecular Plant-Microbe Interactions,2018,31(10):995-1005.
[24] Figueroa C M,Lunn J E. A tale of two sugars:trehalose 6-phosphate and sucrose[J]. Plant Physiology,2016,172(1):7-27.
[25] Nardozza S,Boldingh H L,Kashuba M P,et al. Carbon starvation reduces carbohydrate and anthocyanin accumulation in red-fleshed fruit via trehalose 6-phosphate and MYB27[J]. Plant,Cell & Environment,2020,43(4):819-835.
[26] Suárez R,Wong A,Ramírez M,et al. Improvement of drought tolerance and grain yield in common bean by overexpressing trehalose-6-phosphate synthase in rhizobia[J]. Molecular Plant-Microbe Interactions,2008,21(7):958-966.
[27] Sastry V M V S,Rao G R K. Dioctyl phthalate,and antibacterial compound from the marine brown alga-Sargassum wightii[J]. Journal of Applied Phycology,1995,7(2):185-186.
[28] Bazes A,Silkina A,Douzenel P,et al. Investigation of the antifouling constituents from the brown alga Sargassum muticum(Yendo) Fensholt[J]. Journal of Applied Phycology,2009,21(4):395-403.
[29] Mendes R,Kruijt M,de Bruijn I,et al. Deciphering the rhizosphere microbiome for disease-suppressive bacteria[J]. Science,2011,332(6033):1097-1100.

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备注/Memo

备注/Memo:
收稿日期:2020-08-04。
基金项目:中国林业科学研究院基本科研业务费专项资助项目(CAFYBB2017MA020);国家自然科学基金项目(31700548)
作者简介:陈淋,助理研究员,研究方向为植物与微生物互作,E-mail:chenlin021390@163.com。
通信作者:刘云鹏,副研究员,研究方向为植物与微生物互作,E-mail:liuyunpeng@caas.cn。
更新日期/Last Update: 1900-01-01