[1]冯丹,张民扬,田时祎,等.日粮添加壳聚糖螯合锌对大肠杆菌攻毒断奶大鼠回肠形态结构及屏障功能的影响[J].南京农业大学学报,2021,44(3):541-547.[doi:10.7685/jnau.202002024]
 FENG Dan,ZHANG Minyang,TIAN Shiyi,et al.The effects of dietary supplementation with chitosan-chelated zinc on the ileal morphology and barrier function in weaned rats challenged with Escherichia coli[J].Journal of Nanjing Agricultural University,2021,44(3):541-547.[doi:10.7685/jnau.202002024]
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日粮添加壳聚糖螯合锌对大肠杆菌攻毒断奶大鼠回肠形态结构及屏障功能的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
44卷
期数:
2021年3期
页码:
541-547
栏目:
动物科学
出版日期:
2021-05-10

文章信息/Info

Title:
The effects of dietary supplementation with chitosan-chelated zinc on the ileal morphology and barrier function in weaned rats challenged with Escherichia coli
作者:
冯丹 张民扬 田时祎 汪晶 朱伟云
南京农业大学动物科技学院/国家动物消化道营养国际联合研究中心, 江苏 南京 210095
Author(s):
FENG Dan ZHANG Minyang TIAN Shiyi WANG Jing ZHU Weiyun
College of Animal Science and Technology/National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China
关键词:
壳聚糖螯合锌大肠杆菌大鼠回肠屏障功能
Keywords:
chitosan-chelated zincEscherichia coliratileumbarrier function
分类号:
S816.2
DOI:
10.7685/jnau.202002024
摘要:
[目的] 本试验旨在探讨壳聚糖螯合锌对大肠杆菌攻毒断奶大鼠回肠形态结构、屏障功能和炎症反应的影响。[方法] 选用48只断奶SD大鼠(初始平均体重55.65 g),分为4组:空白对照组(N-CON)、攻毒对照组(CON)、硫酸锌组(ZnSO4)和壳聚糖螯合锌组(CS-Zn)。正式试验开始,N-CON和CON组大鼠饲喂基础日粮,ZnSO4组大鼠饲喂添加含50 mg·kg-1 Zn的硫酸锌日粮,CS-Zn组大鼠饲喂添加640 mg·kg-1壳聚糖螯合锌颗粒(锌含量50 mg·kg-1)的日粮。试验期为14 d,试验第9~12天,CON、ZnSO4和CS-Zn组每天灌胃4 mL总菌数量为1×1010 CFU·mL-1的大肠杆菌菌液。[结果] 与N-CON组相比,CON组大鼠回肠绒毛高度以及绒毛高度/隐窝深度显著降低,隐窝深度以及髓过氧化物酶(MPO)活性显著增加,炎症因子TNF-α、IL-6和IL-1β水平显著增加,IL-10浓度显著降低。而与CON组相比,CS-Zn组大鼠绒毛高度以及绒毛高度/隐窝深度显著上升,隐窝深度显著降低;肠黏膜紧密连接蛋白基因ZO-1、Occludin、Claudin-1表达量显著上升,组织MPO活性显著降低,促炎因子TNF-α和IL-6水平显著降低,抑炎因子IL-10水平显著升高,TLR-4信号通路相关分子基因MyD88、IRAK1、TRAF6表达量明显下调,MyD88蛋白表达量显著下降;有益菌Lactobacillus的数量显著上升。[结论] 壳聚糖螯合锌可显著改善大肠杆菌攻毒大鼠的回肠形态结构,并且可通过上调有益菌含量及抑制TLR-4信号通路,降低炎症细胞因子水平,降低炎症反应,保护肠黏膜屏障功能。
Abstract:
[Objectives] This experiment was conducted to investigate the effects of chitosan-chelated zinc(CS-Zn) on the ileal morphology,barrier function and inflammatory response in rats challenged with Escherichia coli. [Methods] Forty-eight weaned SD rats(with an initial mean body weight of 55.65 g) were divided into four groups:blank control group(N-CON),challenge control group(CON),zinc sulfate group(ZnSO4) and chitosan-chelated zinc group(CS-Zn). At the beginning of the formal experiment,the rats of N-CON group and CON group were fed basic diet,the rats of ZnSO4 group were fed a zinc sulfate diet containing 50 mg·kg-1 zinc and the rats of CS-Zn group were fed a diet containing 640 mg·kg-1 chitosan chelated zinc granules(containing 50 mg·kg-1 zinc). The experimental period was 14 days,and on the 9th to 12th day,the rats of CON group,ZnSO4 group and CS-Zn group were given 4 mL of E.coli with total bacteria count of 1×1010 CFU·mL-1. [Results] Compared with N-CON group,the ileal villus height and villus height/crypt depth were significantly reduced,crypt depth and myeloperoxidase(MPO) activity and the levels of inflammatory factors TNF-α,IL-6 and IL-1β significantly increased,and the level of IL-10 decreased in the CON group. However,compared with CON group,the villus height and villus height/crypt depth increased,while the crypt depth decreased significantly in CS-Zn group. The gene expression level of intestinal mucosa tight junction protein ZO-1,Occludin and Claudin-1 and anti-inflammatory cytokines IL-10 level increased,while MPO activity and pro-inflammatory cytokines TNF-α and IL-6 decreased in CS-Zn group. In addition,expression level of TLR-4 signal pathway relavant molecular MyD88,IRAK1 and TRAF6 and protein expression level of MyD88 decreased in the CS-Zn group. CS-Zn significantly increased the number of the beneficial bacteria Lactobacillus. [Conclusions] Chitosan-chelated zinc can significantly improve the ileal morphology structure of E.coli-infected rats,and reduce inflammatory cytokines by up-regulating the number of the beneficial bacteria,and inhibiting the TLR-4 signaling pathway,thereby reducing inflammatory response and protecting the intestinal mucosal barrier function.

参考文献/References:

[1] Turner J R. Molecular basis of epithelial barrier regulation:from basic mechanisms to clinical application[J]. The American Journal of Pathology,2006,169(6):1901-1909.
[2] Turner J R. Intestinal mucosal barrier function in health and disease[J]. Nature Reviews Immunology,2009,9(11):799-809.
[3] Zhang B K,Shao Y X,Liu D,et al. Zinc prevents Salmonella enterica serovar typhimurium-induced loss of intestinal mucosal barrier function in broiler chickens[J]. Avian Pathology,2012,41(4):361-367.
[4] Wong C P,Ho E. Zinc and its role in age-related inflammation and immune dysfunction[J]. Molecular Nutrition & Food Research,2012,56(1):77-87.
[5] Yin H,Du Y G,Zhang J Z. Low molecular weight and oligomeric chitosans and their bioactivities[J]. Current Topics in Medicinal Chemistry,2009,9(16):1546-1559.
[6] Goiri I,Oregui L M,Garcia-Rodriguez A. Use of chitosans to modulate ruminal fermentation of a 50:50 forage-to-concentrate diet in sheep[J]. Journal of Animal Science,2010,88(2):749-755.
[7] 谢正军,朱叶萌,杜美丹,等. 壳聚糖锌对断奶仔猪生长性能、血清激素和生化指标的影响[J]. 动物营养学报,2010,22(5):1355-1360. Xie Z J,Zhu Y M,Du M D,et al. Effects of chitosan-zinc on growth performance,serum hormone and biochemical indices of weanling piglets[J]. Chinese Journal of Animal Nutrition,2010,22(5):1355-1360(in Chinese with English abstract).
[8] 谢正军,刘国花,李云涛,等. 壳聚糖锌对断奶仔猪小肠组织学形态与功能的影响[J]. 中国畜牧杂志,2012,48(1):32-37. Xie Z J,Liu G H,Li Y T,et al. Effects of CS-Zn on histological morphology and function of small intestine in piglets[J]. Chinese Journal of Animal Science,2012,48(1):32-37(in Chinese with English abstract).
[9] Han X Y,Ma Y F,Lv M Y,et al. Chitosan-zinc chelate improves intestinal structure and mucosal function and decreases apoptosis in ileal mucosal epithelial cells in weaned pigs[J]. The British Journal of Nutrition,2014,111(8):1405-1411.
[10] Hu L S,Cheng S S,Li Y,et al. Chitosan-Zn chelate downregulates TLR4-NF-κB signal pathway of inflammatory response and cell death-associated proteins compared to inorganic zinc[J]. Biological Trace Element Research,2018,184(1):92-98.
[11] 刘壮,慕春龙,彭宇,等. 极低蛋白日粮补充不同形式氮营养素对生长猪回肠食糜菌群、代谢产物和屏障功能的影响[J]. 南京农业大学学报,2019,42(3):526-534. DOI:10.7685/jnau.201806015. Liu Z,Mu C L,Peng Y,et al. Effects of extremely-low-protein diets supplemented with different nitrogen source on ileal microbial communities,metabolites profiles and barrier function of growing pigs[J]. Journal of Nanjing Agricultural University,2019,42(3):526-534(in Chinese with English abstract).
[12] 田时祎,王珏,汪晶,等. 早期低聚半乳糖干预对哺乳仔猪回肠形态、功能发育相关基因及回肠菌群的影响[J]. 南京农业大学学报,2018,41(5):917-924. DOI:10.7685/jnau.201711031. Tian S Y,Wang J,Wang J,et al. The effect of early intervention with galacto-oligosaccharides on the morphological structure,functional development,and microbial community in ileum of suckling piglets[J]. Journal of Nanjing Agricultural University,2018,41(5):917-924(in Chinese with English abstract).
[13] Montagne L,Pluske J R,Hampson D J. A review of interactions between dietary fibre and the intestinal mucosa,and their consequences on digestive health in young non-ruminant animals[J]. Animal Feed Science and Technology,2003,108(1/2/3/4):95-117.
[14] da Silva M S,Sánchez-Fidalgo S,Talero E,et al. Anti-inflammatory intestinal activity of Abarema cochliacarpos(Gomes) barneby & grimes in TNBS colitis model[J]. Journal of Ethnopharmacology,2010,128(2):467-475.
[15] Berkes J,Viswanathan V K,Savkovic S D,et al. Intestinal epithelial responses to enteric pathogens:effects on the tight junction barrier,ion transport,and inflammation[J]. Gut,2003,52(3):439-451.
[16] 崔君,傅春妮,李留安,等. 褐藻多糖对仔猪抗氧化能力、肠道菌群和细胞因子的影响[J]. 畜牧与兽医,2019,51(12):66-69. Cui J,Fu C N,Li L A,et al. Effects of brown algae polysaccharide on growth,antioxidantcapacity,intestinal flora and cytokines in piglets[J]. Animal Husbandry and Veterinary Medicine,2019,51(12):66-69.
[17] 厉成敏,朱乐乐,李莲,等. 重组牛脂多糖结合蛋白对脂多糖诱导的奶牛乳腺炎症反应指标的影响[J]. 南京农业大学学报,2018,41(4):715-721. DOI:10.7685/jnau.201711022. Li C M,Zhu L L,Li L,et al. Effect of recombinant bovine lipopolysaccharide(rbLBP) on indicators of inflammation in lipopolysaccharide induced dairy cows[J]. Journal of Nanjing Agricultural University,2018,41(4):715-721(in Chinese with English abstract).
[18] Long L N,Chen J S,Zhang Y G,et al. Comparison of porous and nano zinc oxide for replacing high-dose dietary regular zinc oxide in weaning piglets[J]. PLoS One,2017,12(8):e0182550.
[19] 张丽娜. PRRSV感染激活IL-10产生的信号通路[D]. 武汉:华中农业大学,2012. Zhang L N. Signaling pathway of IL-10 production actived by PRRSV infection[D]. Wuhan:Huazhong Agricultural University,2012(in Chinese with English abstract).
[20] Wan Y,Fu Y Q,Wang F L,et al. Protective effects of a lipid extract from hard-shelled mussel(Mytilus coruscus) on intestinal integrity after lipopolysaccharide challenge in mice[J]. Nutrients,2018,10(7):860.
[21] Lu Y C,Yeh W C,Ohashi P S. LPS/TLR4 signal transduction pathway[J]. Cytokine,2008,42(2):145-151.

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备注/Memo

备注/Memo:
收稿日期:2020-02-22。
基金项目:中央高校基本科研业务费专项资金(KYZ201722)
作者简介:冯丹,硕士研究生。
通信作者:汪晶,教授,博导,主要从事单胃动物营养研究,E-mail:jwang8@njau.edu.cn。
更新日期/Last Update: 1900-01-01