[1]王智博,姚晓磊,张红琳,等.不同产羔数和发情时期湖羊代谢产物及肠道菌群结构的差异分析研究[J].南京农业大学学报,2021,44(3):548-553.[doi:10.7685/jnau.202003019]
 WANG Zhibo,YAO Xiaolei,ZHANG Honglin,et al.Changes of rectal contents microbiota and their metabolites associated with prolificacy and estrus cycle in Hu sheep[J].Journal of Nanjing Agricultural University,2021,44(3):548-553.[doi:10.7685/jnau.202003019]
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不同产羔数和发情时期湖羊代谢产物及肠道菌群结构的差异分析研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
44卷
期数:
2021年3期
页码:
548-553
栏目:
动物科学
出版日期:
2021-05-10

文章信息/Info

Title:
Changes of rectal contents microbiota and their metabolites associated with prolificacy and estrus cycle in Hu sheep
作者:
王智博1 姚晓磊1 张红琳2 张艳丽1
1. 南京农业大学江苏省家畜胚胎工程实验室, 江苏 南京 210095;
2. 南京晓庄学院食品科学学院, 江苏 南京 211171
Author(s):
WANG Zhibo1 YAO Xiaolei1 ZHANG Honglin2 ZHANG Yanli1
1. Jiangsu Livestock Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China;
2. School of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China
关键词:
湖羊繁殖力肠道微生物挥发性脂肪酸(VFA)
Keywords:
Hu sheepprolificacyintestinal microfloravolatile fatty acid(VFA)
分类号:
S826.8
DOI:
10.7685/jnau.202003019
摘要:
[目的] 本试验旨在探究不同繁殖力湖羊直肠内容物代谢产物及肠道菌群结构的变化特点,分析湖羊繁殖性能与肠道微生物间的相关性。[方法] 选取体型均一、生理状态相似的连续三胎产三羔(高繁组,HP组)和产单羔(低繁组,LP组)空怀湖羊各6只,每组3只,对其进行同期发情处理,分别在撤栓时(发情前,LP1组、HP1组)和发情时(LP2组、HP2组)采集直肠内容物,测量其挥发性脂肪酸的含量,并采用高通量测序技术对其直肠微生物进行分析。[结果] 发情前,LP组湖羊乙酸含量显著低于HP组(P<0.05);LP组湖羊在发情前直肠内容物的丙酸和丁酸含量显著低于发情时(P<0.05),HP组湖羊在发情时的乙酸、丙酸和丁酸含量显著高于发情前(P<0.05)。高通量测序分析结果显示:在发情时,LP组与HP组湖羊菌群结构差异显著(P<0.05);β多样性的箱型图分析显示LP组和HP组湖羊菌群结构差异在发情时与发情前均极显著(P<0.01)。[结论] 不同繁殖力和发情时期湖羊的肠道微生物及代谢产物呈现较明显的差异性,为湖羊繁殖力与肠道菌群关系的研究提供了理论基础。
Abstract:
[Objectives] This study aimed to explore the characteristics of Hu sheep fecal metabolites and intestinal microflora structure,and to analyze the correlation between the reproductive performance and intestinal microflora. [Methods] A total of ten Hu sheeps with identical lambing records(3 records) were selected for estrus treatment at the same time,and divided into a high prolificacy group(HP,litter size=3) and a low prolificacy group(LP,litter size=1). The feces samples were collected before estrus or during estrus,respectively. Then the volatile fatty acid(VFA) content was measured,and the intestinal microorganism was analyzed by high-throughput sequencing. [Results] Before estrus,the content of acetic acid in LP group was significantly lower than that in HP group(P<0.05). The content of propionic acid and butyric acid in LP group was significantly lower than that of HP group before estrus(P<0.05);the contents of acetic acid,propionic acid and butyric acid during estrus in HP group were significantly higher than those before estrus(P<0.05). The results of high-throughput sequencing showed that there was a significant difference in the structure of the flora between the LP group and the HP group(P<0.05),and the difference between the LP group and the HP group was significant(P<0.01) at the peak of estrus and before estrus period. [Conclusions] There are obvious differences in intestinal microorganisms and metabolites of Hu sheep in different fecundity and estrous periods,which indicate that there may be a certain correlation between reproductive performance and intestinal microbial changes of Hu sheep.

参考文献/References:

[1] Andreas K. 影响猪的肠道菌群的日粮营养策略[J]. 中国畜牧兽医,2006,33(4):63-65. AndreasK. Dietary nutrition strategies affecting pig intestinal flora[J]. China Animal Husbandry & Veterinary Medicine,2006,33(4):63-65(in Chinese).
[2] 赵桂英,杨亮宇,段纲,等. 断奶仔猪胃肠道正常菌群的数量和分区[J]. 黑龙江畜牧兽医,2003(9):26-27. Zhao G Y,Yang L Y,Duan G,et al. Number and division of normal bacterial flora in weaning piglets[J]. Heilongjiang Journal of Animal Science and Veterinary Medicine,2003(9):26-27(in Chinese).
[3] Hang S Q,Zhu W Y. Gut bacterial and lactobacilli communities of weaning piglets in response to mannan oligosaccharide and sugar beet pulp in vitro fermentation[J]. Journal of Integrative Agriculture,2012,11(1):122-133.
[4] Kwa M,Plottel C S,Blaser M J,et al. The intestinal microbiome and estrogen receptor-positive female breast cancer[J]. JNCI:Journal of the National Cancer Institute,2016,108(8):djw029.
[5] Moreno-Indias I,Sánchez-Alcoholado L,Sánchez-Garrido Má,et al. Neonatal androgen exposure causes persistent gut microbiota dysbiosis related to metabolic disease in adult female rats[J]. Endocrinology,2016,157(12):4888-4898.
[6] Baker J M,Al-Nakkash L,Herbst-Kralovetz M M. Estrogen-gut microbiome axis:physiological and clinical implications[J]. Maturitas,2017,103:45-53.
[7] Kritas S K,Marubashi T,Filioussis G,et al. Reproductive performance of sows was improved by administration of a sporing bacillary probiotic(Bacillus subtilis C-3102)[J]. Journal of Animal Science,2015,93(1):405-413.
[8] 唐义梅. 梅山猪粪便微生物移植对长×大后备母猪卵泡发育的影响[D]. 武汉:华中农业大学,2019. Tang Y M. Effect of fecal microbiota transplantation of Meishanpig on follicle development in Landrace×Yorkshire gilts[D]. Wuhan:Huazhong Agricultural University,2019(in Chinese with English abstract).
[9] Yu L,Williams B A,Tamminga S,et al. In vitro fermentation kinetics of some non-digestible carbohydrates by the caecal microbial community of broilers[J]. Animal Feed Science and Technology,2005,123/124:687-702.
[10] 刘文涛,李晓斌,臧长江,等. 饲喂不同结构性与非结构性碳水化合物比例日粮对绵羊瘤胃发酵参数的影响[J]. 饲料工业,2019,40(24):18-24. Liu W T,Li X B,Zang C J,et al. Effects of dietary diets with different ratios of structural and non-structural carbohydrates on rumen fermentation parameters in sheep[J]. Feed Industry,2019,40(24):18-24(in Chinese with English abstract).
[11] Olszak T,An D,Zeissig S,et al. Microbial exposure during early life has persistent effects on natural killer T cell function[J]. Science,2012,336(6080):489-93.
[12] Su Y,Yao W,Perez-Gutierrez O N,et al. 16S ribosomal RNA-based methods to monitor changes in the hindgut bacterial community of piglets after oral administration of Lactobacillus sobrius S1[J]. Anaerobe,2008,14(2):78-86.
[13] 王欢,杨密,蒲广,等. 日粮纤维水平对苏淮猪直肠中氮、磷含量及结肠菌群数量的影响[J]. 畜牧与兽医,2020,52(6):45-49. Wang H,Yang M,Pu G,et al. Effects of fiber levels in diet on rectal nitrogen and phosphorus contents and colonic bacteria number in Suhuaipigs[J]. Animal Husbandry & Veterinary Medicine,2020,52(6):45-49(in Chinese with English abstract).
[14] 韦玥瑞,杨硕,王月,等. 季节对内蒙古白绒山羊瘤胃甲烷产生量及相关微生物数量的影响[J]. 畜牧与兽医,2020,52(4):24-30. Wei Y R,Yang S,Wang Y,et al. Effects of seasons on methane production and related microbial quantity in the rumen of Inner Mongolia White Cashmere goats[J]. Animal Husbandry & Veterinary Medicine,2020,52(4):24-30(in Chinese with English abstract).
[15] Ding N,Zhang X,Zhang X D,et al. Impairment of spermatogenesis and sperm motility by the high-fat diet-induced dysbiosis of gut microbes[J]. Gut,2020,69(9):1608-1619.
[16] Gao X X,Yao X L,Yang H,et al. Role of FGF9 in sheep testis steroidogenesis during sexual maturation[J]. Animal Reproduction Science,2018,197:177-184.
[17] Zhou J W,Ji K X,Liu H,et al. Effect of air temperature on growth performance,apparent digestibilities,rumen fermentation and serum metabolites in Altay and Hu lambs[J]. Journal of Animal Physiology and Animal Nutrition,2020,104(4):1024-1033.
[18] 李宗凯,陆扬,刘家俊,等. 益生菌对生长育肥猪生长性能、肉品质和结肠菌群的影响[J]. 南京农业大学学报,2020,43(3):523-528. DOI:10.7685/jnau.201906025. Li Z K,Lu Y,Liu J J,et al. Effects of probiotics on the growth performance,meat quality and colonic microflora of growing and finishingpigs[J]. Journal of Nanjing Agricultural University,2020,43(3):523-528(in Chinese with English abstract).
[19] Ervin S M,Li H,Lim L,et al. Gut microbial β-glucuronidases reactivate estrogens as components of the estrobolome that reactivate estrogens[J]. Journal of Biological Chemistry,2019,294(49):18586-18599.
[20] Acharya K D,Gao X,Bless E P,et al. Estradiol and high fat diet associate with changes in gut microbiota in female ob/obmice[J]. Scientific Reports,2019,9:20192.
[21] Kaczynski P,Bauersachs S,Baryla M,et al. Estradiol-17β-induced changes in the porcine endometrial transcriptome in vivo[J]. International Journal of Molecular Sciences,2020,21(3):890.
[22] 张金璧,林飞,潘增祥,等. 猪不同程度闭锁卵泡判定方法的比较研究[J]. 南京农业大学学报,2013,36(1):115-119. DOI:10.7685/j.issn.1000-2030.2013.01.020. Zhang J B,Lin F,Pan Z X,et al. Comparative study of methods to determine the follicular atresia extent in pigs[J]. Journal of Nanjing Agricultural University,2013,36(1):115-119(in Chinese with English abstract).
[23] Kudo H,Oki Y. Microtus species as new herbivorous laboratory animals:Reproduction;Bacterial flora and fermentation in the digestive tracts;And nutritional physiology[J]. Veterinary Research Communications,1984,8(1):77-91.
[24] 邹冰洁. 粗纤维对母猪产仔性能的影响[D]. 南京:南京农业大学,2014. Zou B J. Effects of dietary fiber on the farrowing performance of sows[D]. Nanjing:Nanjing Agricultural University,2014(in Chinese with English abstract).

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备注/Memo

备注/Memo:
收稿日期:2020-03-09。
基金项目:江苏现代农业(肉羊)产业技术体系建设专项(JATS〔2020〕427)
作者简介:王智博,博士研究生。
通信作者:张艳丽,教授,研究方向为肉羊遗传育种与繁殖,E-mail:zhangyanli@njau.edu.cn。
更新日期/Last Update: 1900-01-01