[1]訾玉祥,陆兆新,吕风霞,等.紫苏叶中甘油糖脂的抗氧化活性研究[J].南京农业大学学报,2021,44(3):561-567.[doi:10.7685/jnau.202007033]
 ZI Yuxiang,LU Zhaoxin,Lü Fengxia,et al.The study of antioxidant activities of glycoglycerolipids in leaves of Perilla frutescens[J].Journal of Nanjing Agricultural University,2021,44(3):561-567.[doi:10.7685/jnau.202007033]
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紫苏叶中甘油糖脂的抗氧化活性研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
44卷
期数:
2021年3期
页码:
561-567
栏目:
食品与工程
出版日期:
2021-05-10

文章信息/Info

Title:
The study of antioxidant activities of glycoglycerolipids in leaves of Perilla frutescens
作者:
訾玉祥 陆兆新 吕风霞 别小妹 张充 赵海珍
南京农业大学食品科学技术学院, 江苏 南京 210095
Author(s):
ZI Yuxiang LU Zhaoxin Lü Fengxia BIE Xiaomei ZHANG Chong ZHAO Haizhen
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
关键词:
紫苏甘油糖脂抗氧化活性结构-活性关系
Keywords:
Perilla frutescensglycoglycerolipidsantioxidant activitystructure-activity relationship
分类号:
TS201.2
DOI:
10.7685/jnau.202007033
摘要:
[目的] 本文旨在研究紫苏叶中3种不同分子结构甘油糖脂的抗氧化活性并初步探讨其活性构效关系。[方法] 通过化学评价法评价甘油糖脂的2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)、1,1-二苯基-2-三硝基苯肼(DPPH)、羟自由基(·OH)及超氧阴离子(O2·-)清除能力并测定其还原力,采用细胞氧化损伤模型研究甘油糖脂对抗氧化酶活性的影响。[结果] 从紫苏叶中分离纯化获得了3种甘油糖脂单体,即MGDG(C18:3/C18:3)、DGDG(C18:3/C18:3)和DGDG(C18:3/C16:0)。MGDG(C18:3/C18:3)和DGDG(C18:3/C18:3)的抗氧化能力强于DGDG(C18:3/C16:0)。3种甘油糖脂均可抑制ABTS自由基、DPPH自由基、·OH、O2·-的产生及提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)的活性,且具有较强的还原力。[结论] 3种甘油糖脂均具有抗氧化能力,但脂肪酰基组成不同的甘油糖脂具有不同的抗氧化能力,其中,脂酰基及糖基数量对甘油糖脂类化合物的抗氧化活性影响较大。
Abstract:
[Objectives] This article aimed to study the antioxidant activities of three different molecular structures of glycoglycerolipids in leaves of Perilla frutescens and the structure-activity relationship was preliminarily discussed. [Methods] The scavenging abilities of 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid(ABTS),1,1-diphenyl-2-picrylhydrazyl(DPPH),hydroxyl radicals(·OH) and superoxide anion radicals(O2·-) were measured by chemical evaluation method,and the cell oxidative damage model was used to study the effects of glycoglycerolipids on the activities of antioxidant enzymes. [Results] Three glycoglycerolipids monomers were separated and purified from leaves of P. frutescens,including MGDG(C18:3/C18:3),DGDG(C18:3/C18:3) and DGDG(C18:3/C16:0). The antioxidant activities of MGDG(C18:3/C18:3) and DGDG(C18:3/C18:3) were higher than that of DGDG(C18:3/C16:0). All glycoglycerolipids presented strong reducing power,and they could not only inhibit the production of ABTS,DPPH,·OH and O2·-,but also significantly enhance the activities of superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GSH-Px). [Conclusions] Glycoglycerolipids with different fatty acyl composition presented different antioxidant activity,and the preliminary study of structure-activity relationship showed that the saturation degree of fatty acyl group and the number of sugar groups greatly affected the antioxidant activities of glycoglycerolipids.

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备注/Memo

备注/Memo:
收稿日期:2020-07-21。
基金项目:国家自然科学基金项目(31771950,31301558);江苏高校优势学科建设工程资助项目
作者简介:訾玉祥,硕士研究生。
通信作者:赵海珍,教授,主要从事酶和微生物生物催化转化制备功能性物质、天然功能性物质的分离提取及其活性机制研究和油脂改良研究,E-mail:zhaohz@njau.edu.cn。
更新日期/Last Update: 1900-01-01